A kind of pathogenic nucleic acid and drug resistance gene detection kit and its application

A gene detection and drug resistance technology, applied in the field of medical biology, can solve the problems of inability to detect multiple target genes or pathogens, and lack of mycoplasma drug resistance detection reagents, etc., to achieve rapid and objective detection results, strong repeatability, and reduce Opportunistic Effects of Pollution

Active Publication Date: 2017-12-29
常州百代生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the products currently on the market are single-channel detection, which can only perform single-plex PCR reactions. One PCR reaction tube can only detect one gene or pathogen in one reaction, and cannot specifically detect multiple target genes or pathogens, and Mycoplasma resistance detection reagents without clinically relevant methods

Method used

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  • A kind of pathogenic nucleic acid and drug resistance gene detection kit and its application
  • A kind of pathogenic nucleic acid and drug resistance gene detection kit and its application
  • A kind of pathogenic nucleic acid and drug resistance gene detection kit and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0064] The kit includes the following components:

[0065] PCR reaction solution, enzyme mixture, Chlamydia trachomatis / Ureaplasma urealyticum / Mycoplasma hominis / drug resistance gene multiple reaction solution, positive control one, negative control;

[0066] The PCR reaction solution includes 10× buffer, 25mM MgCl2, 10mM dUTP and 10mM dNTPs;

[0067] The enzyme mixture includes Taq enzyme and UNG enzyme, the Taq enzyme is a hot-start Taq enzyme, and the UNG enzyme is uracil-N-glycosylase.

[0068] The ratio of the upstream and downstream primers and probes of the Chlamydia trachomatis / Ureaplasma urealyticum / Mycoplasma hominis / drug resistance gene multiple reaction solution is 6:4:2; the preferred upstream primer is 600nM, the downstream primer is 400nM, and the probe It is 200nM.

[0069] Refer to Table 1 for the nucleotide sequences of the primers and probes of Mycoplasma hominis, Ureaplasma Urealyticum, Chlamydia trachomatis and Ureaplasma Ureaplasma tetracycline resistance gene use...

Embodiment 2

[0109] The kit includes the following components:

[0110] PCR reaction solution, enzyme mixture, Chlamydia trachomatis / Ureaplasma / Mycoplasma hominis multiple reaction solution, drug resistance gene reaction solution, positive control 1, positive control 2, negative control;

[0111] The PCR reaction solution includes 10× buffer, 25mM MgCl2, 10mM dUTP and 10mM dNTPs;

[0112] The enzyme mixture includes Taq enzyme and UNG enzyme. The Taq enzyme is a hot-start Taq enzyme, and the UNG enzyme is uracil-N-glycosylase.

[0113] The ratio of the upstream and downstream primers and probes of the Chlamydia trachomatis / Ureaplasma urealyticum / Mycoplasma hominis multiple reaction solution is 6:4:2; the preferred upstream primer is 600 nM, the downstream primer is 400 nM, and the probe is 200 nM.

[0114] The ratio of the upstream and downstream primers and probes of the drug resistance gene reaction solution is 6:4:2; the preferred upstream primer is 600 nM, the downstream primer is 400 nM, and th...

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Abstract

The invention relates to the field of medical biotechnology, in particular to a detection kit for pathogenic nucleic acid and drug resistance gene and its application technology. The kit designed by the present invention includes the following two components: PCR reaction buffer, enzyme mixture, Chlamydia trachomatis / Ureaplasma urealyticum / Mycoplasma hominis / drug resistance gene multiple reaction solution; or PCR reaction buffer, enzyme mixture, Chlamydia trachomatis / Ureaplasma urealyticum / Mycoplasma hominis multiple reaction solution, drug resistance gene reaction solution. The kit of the present invention adopts multiple fluorescent quantitative PCR technology, can simultaneously detect multiple target genes in the same PCR reaction tube, and provides strong technical support for simultaneous detection of pathogens and their drug resistance genes. The method has the characteristics of less sample requirement, low cost, simple operation, high sensitivity and good specificity, and has great social and economic significance.

Description

Technical field [0001] The present invention relates to the field of medical biotechnology, in particular to a pathogen nucleic acid and drug resistance gene detection kit and its application, and in particular to a kit for Chlamydia trachomatis / Ureaplasma / Mycoplasma hominis and drug resistance genes and the same application. Background technique [0002] Sexually transmitted diseases (STD) are a group of global infectious diseases, which are mainly spread through sexual contact. Some STDs can be spread through non-sexual contact, such as sharing needles, breastfeeding or even contact. [0003] The incidence of STD in my country is currently on the rise. Among them, genitourinary tract infections are caused by three pathogens: Chlamydiatrachomatis (CT), Mycoplasma hominis (M.humenis, MH) and Ureaplasmaurealyticum (UU) , Has become a common disease in the department of gynecology, dermatology, and genitourinary departments in hospitals, and is a key target of prevention and treatmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/35C12R1/01
Inventor 孔祥宾夏国庆丁燕芬朱啸悦
Owner 常州百代生物科技股份有限公司
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