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Kit for detecting mycoplasma urealytium and human mycoplasma

A technology of Mycoplasma hominis and Ureaplasma urealyticum, which is applied in the fields of biochemical equipment and methods, and the measurement/testing of microorganisms, can solve the problems that are not easy to realize, and achieve the effects of simple operation, easy popularization, high sensitivity and specificity

Active Publication Date: 2017-01-11
AUTOBIO DIAGNOSTICS CO LTD
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Problems solved by technology

The PCR method is a relatively advanced technology at present, but because the PCR method sample needs to be washed, high-temperature extraction of DNA and other complex processes, it has high requirements for the operating environment and operators, and requires a large-scale instrument PCR instrument, so it is currently difficult to implement clinically

Method used

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Embodiment Construction

[0014] One, the preparation of kit of the present invention.

[0015] 1. Preparation of Mycoplasma Identification Card

[0016] Weigh L-proline p-nitroaniline, dissolve it with 0.4M Tris-citrate buffer (pH8.5) to make the concentration reach 1.5mg / ml, mix well, and obtain proline aminopeptidase detection reagent. The liquid reagent is added to a 6.0mm diameter circular paper at the rate of 5 ul / sheet, and dried overnight to obtain the proline aminopeptidase immobilized detection test paper.

[0017] Weigh L-leucine-p-nitroaniline and wash with 0.2M NaH 2 PO 4 -Na 2 HPO 4 The buffer solution (pH6.6) was dissolved to make the concentration reach 2.0 mg / ml, and mixed evenly to obtain the leucine aminopeptidase detection reagent. The liquid reagent is added to a 6.0mm diameter circular paper at the rate of 5 ul / sheet, and dried overnight to obtain the leucine aminopeptidase immobilized detection test paper.

[0018] Weigh 5-bromo-6-chloro-3-indole-α-D-glucoside in 0.4 M Na c...

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Abstract

The invention discloses a kit for detecting mycoplasma urealytium and human mycoplasma. The kit comprises four detection reagents: a compound of L-proline and enzyme substrate as chromogen for detecting activity of proline aminopeptidase, a compound of L-leucine and enzyme substrate as chromogen for detecting activity of leucine aminopeptidase, a compound of alpha-D-glucoside and enzyme substrate as chromogen for detecting activity of alpha-glucosidase and a compound of neuraminic acid and enzyme substrate as chromogen for detecting activity of neuraminidase, wherein the chromogen is aniline, naphthaline, naphthol, indoxyl and derivative thereof. According to the invention, a dry chemical enzyme method is adopted for detecting the enzyme compounded in the growth process or the enzyme generated by stimulating host cells, so that the purpose of detecting and identifying mycoplasma urealytium and human mycoplasma can be achieved; and the kit has the advantages of high speed, convenience, accuracy, and the like.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a kit for detecting ureaplasma urealyticum and mycoplasma hominis. Background technique [0002] Mycoplasma is the smallest and simplest prokaryotic microorganism found so far that is similar to bacteria but does not have a cell wall. It is one of the main pathogenic bacteria that cause human non-gonococcal urethritis (NGU), cervicitis, pelvic inflammatory disease, orchitis, epididymitis and other diseases , and can lead to male and female infertility, these pathogens can invade and destroy the epithelial cells of the urogenital tract mucosa, and are more likely to cause other secondary disease infections. Clinically, the mycoplasmas that cause venereal diseases are mainly ureaplasma urealyticum (UU) and mycoplasma hominis (MH). Survey statistics in recent years have shown that the incidence of sexually transmitted diseases has been on the rise, and drug resistance is also in...

Claims

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Application Information

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IPC IPC(8): C12Q1/37C12Q1/34C12Q1/04
CPCC12Q1/04C12Q1/34C12Q1/37C12Q2334/30C12Q2337/10
Inventor 王则宇杨红云杨森刘志磊秦磊杨静孙武举付光宇吴学伟
Owner AUTOBIO DIAGNOSTICS CO LTD
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