CPCR (convective polymerase chain reaction) amplification detection device based on chemical heating

Abstract

The invention discloses a CPCR (convective polymerase chain reaction) amplification detection device based on chemical heating. The device comprises a chemical heating reactor, a thermal conduction module, a chemical heating pack, a thermal insulation casing, a CPCR tube and a paper strip detection chip; the thermal conduction module is heated by virtue of heat energy which is generated from a reaction between water and the chemical heating pack, so that the bottom of the CPCR reaction tube is heated, and the bottom of the CPCR tube is kept at 95+/-2 DEG C till the end of a CPCR amplification reaction; and after the reaction is finished, the CPCR tube is transferred to the paper strip detection chip and a detection result is judged and read by virtue of human eyes, so that detection is completed. According to the device provided by the invention, isothermal heating required by the CPCR amplification reaction is achieved by virtue of the heat energy generated from the chemical heating, and meanwhile, by judging and reading the detection result by virtue of the human eyes in the combination with paper strip detection, nucleic acid amplification and detection, without external electric power, can be achieve; therefore, a good foundation is laid for conducting accurate, convenient, rapid, low-cost and high-sensitivity disease diagnosis in such environments having no electric power or in lack of the electric power as field or outdoor environments, undeveloped or remote areas and the like.

Description

technical field [0001] The invention relates to the field of life medical detection and diagnosis, in particular to a convective PCR (CPCR) amplification detection device which utilizes chemical heating to realize nucleic acid amplification and detection. Background technique [0002] In traditional nucleic acid diagnosis and analysis, PCR (polymerase chain reaction) reactions often need to be completed by special PCR gene amplification instruments in various medical testing institutions with good basic conditions. Due to the constraints of various external factors such as instruments, devices, and electricity, PCR-based high-sensitivity nucleic acid diagnosis is difficult to promote in areas where various basic conditions are scarce, and it is not suitable for various on-site rapid detection environments. [0003] CPCR achieves rapid nucleic acid amplification through isothermal heating mode. Compared with the high-precision thermal cycle temperature control device required...

AI Technical Summary

Problems solved by technology

Due to the constraints of various external factors such as instruments, devices, and electricity, PCR-based high-sensitivity nucleic acid diagnosis is difficult to promote in areas where various basic conditions are scarce, and is not suitable for various on-site rapid detection environments

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