Extra-high flux unicellular nucleic acid molecule real-time fluorescent quantitation analysis integrated quick detecting system

A real-time fluorescence quantification, single-cell nucleic acid technology, applied in the determination/inspection of microorganisms, biochemical instruments, laboratory tools, etc. It can improve the detection throughput, realize fast drawing, and reduce the calculation effect.
CN110734854AActive Publication Date: 2020-01-31SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
Publication Date
2020-01-31

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Abstract

The invention discloses an extra-high flux unicellular nucleic acid molecule real-time fluorescent quantitation analysis integrated quick detecting system. The extra-high flux unicellular nucleic acidmolecule real-time fluorescent quantitation analysis integrated quick detecting system comprises a micro-fluidic chip, an automatic sample adding device, a temperature control thermocirculator, a fluorescent imaging system and a data storage and analysis system, wherein the automatic sample adding device is provided with freedom degrees in an X axis, a Y axis and a Z axis and is used for automatically adding samples and reagents to the micro-fluidic chip; and the data storage and analysis system is used for analyzing fluorescence signals of the samples which are collected, identifying positive samples and drawing out a real-time fluorescent quantitation analytical curve of the positive samples. The extra-high flux unicellular nucleic acid molecule real-time fluorescent quantitation analysis integrated quick detecting system integrates the micro-fluidic chip, the automatic sample adding device, the temperature control thermocirculator, the fluorescent imaging system and the data storage and analysis system, can realize automatic detection treatment on the samples, can realize unicellular catching nucleic acid amplification in hundreds and millions, and can realize real-time fluorescent quantitation curve analysis.
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Description

technical field

[0001] The invention relates to the technical field of gene detection, in particular to an integrated rapid detection system for real-time fluorescent quantitative analysis of single-cell nucleic acid molecules with ultra-high throughput. Background technique

[0002] The growth, development, differentiation, aging and pathological changes of the body are all related to the differential expression of genes. The occurrence, development and metastasis of tumors are also related to the mutation and differential expression of genes. Cells in the center of tumor tissue, surrounding cells, cells in metastases, etc. also have different functional characteristics due to differences in genome and transcriptional expression profiles, affecting and Determine the outcome of tumor treatment, etc.

[0003] Traditional gene expression research methods usually measure the expression of a gene at the mRNA level. The expression of mRNA level is usually realized by real-time ...

Claims

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