Micro-fluidic chip and detecting device suitable for PCR (polymerase chain reaction) or HRM (high resolution melting) detection analysis

A microfluidic chip and detection device technology, applied in enzymology/microbiology devices, biochemical cleaning devices, bioreactors/fermenters for specific purposes, etc., can solve the problem of different sealing valve functions, cumbersome and time-consuming operations , mutual mixing and interference, etc., to achieve the effect of fast heating, high fluorescence collection efficiency, and high detection sensitivity

Active Publication Date: 2015-01-21
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used sealing forms include pneumatic micro-valve, which is easy to integrate, can realize array sealing, and is easy to operate, but the micro-valve is easy to leak when heated; adopting micro-pillar plug type sealing has good sealing effect, but for array reaction There are many cavities, and the operation is cumbersome and time-consuming; the screw type seal is used, and the sealing situation is the same as that of the micro-plunger type; the liquid curing type seal is used, and the fluid flows into the seal, which may easily cause the cavity reagent to overflow and cause mutual interference. Miscellaneous interference; the wax melting t

Method used

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  • Micro-fluidic chip and detecting device suitable for PCR (polymerase chain reaction) or HRM (high resolution melting) detection analysis
  • Micro-fluidic chip and detecting device suitable for PCR (polymerase chain reaction) or HRM (high resolution melting) detection analysis
  • Micro-fluidic chip and detecting device suitable for PCR (polymerase chain reaction) or HRM (high resolution melting) detection analysis

Examples

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Effect test

example 1

[0048] The cavity size of the combined mold is 33mm×32mm×7mm, the volume of the raised reaction chamber is 12uL, double-row structure, 4 in each row, a total of 8 reaction chambers, the channel is 0.4mm, the diameter of the mold cross column is 1mm, the mold The hole on the cross column is 0.3mm, the diameter of the mold column is 0.3mm, inject PDMS glue into the mold, heat and solidify, remove the mold column, take out the mold cross column, and fall off from the template to obtain a microchannel and reaction chamber. body substrate. The PDMS substrate and the 50mm×40mm glass substrate were cleaned by plasma to modify the surface and bonded to form a microfluidic chip. A 1.2mm rotating long rod with a handle is inserted into the die cross-column hole of the chip. The micro-holes on the mold cross-column are 0.3mm in size, and the micro-holes correspond to the channels generated by the mold post. At this time, the microfluidic chip is processed for biocompatibility, high temp...

example 2

[0050]The combined mold cavity size is 22mm×25mm×7mm, the volume of the raised reaction cavity is 20uL, single-row structure, 2 reaction chambers, the channel is 0.4mm, the diameter of the mold cross column is 1mm, and the hole on the mold cross column is 0.3mm, the diameter of the mold column is 0.3mm, inject PDMS glue into the mold, heat and cure, remove the mold column, take out the mold cross column, and fall off the template to obtain a substrate with microchannels and reaction chambers. The PDMS substrate and the 25mm×30mm glass substrate were cleaned by plasma to modify the surface and bonded to form a microfluidic chip. A 1.2mm rotating long rod with a handle is inserted into the die cross-column hole of the chip. The micro-holes on the mold cross-column are 0.3mm in size, and the micro-holes correspond to the channels generated by the mold post. At this time, the microfluidic chip is processed for biocompatibility, high temperature sterilization and other related proc...

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Abstract

The invention discloses a micro-fluidic chip and a detecting device suitable for PCR (polymerase chain reaction) or HRM (high resolution melting) detection analysis. A micro pipeline and an array reaction chamber base plate form a combined mould, a micro-fluidic base chip is generated by virtue of an injection molding method, the micro-fluidic chip is formed by virtue of plasma cleaning and glass bonding; a rotary rod with a handle and a duct is transversely embedded in an outward channel center of the chip to form a rotary valve; the handle rotates to disconnect the duct to control on-off and sealing of multi-reaction-chamber sampling, so that physical isolation between reaction chambers is realized, the sealing performance is good, and cross contamination does not exist, and thus, a plurality of samples can be detected at the same time. The micro-fluidic chip disclosed by the invention not only can be used for independent PCR detection or HRM detection, but also can be used for performing PCR detection and then performing HRM detection without any change in a combined manner, so that the multi-functionalization of detection is realized.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a microfluidic chip and a detection device suitable for PCR or HRM detection and analysis. Background technique [0002] As a new micro-nano analysis technology platform, the microfluidic chip integrates the basic operation units such as sample preparation, separation and detection involved in the fields of biology and chemistry into a chip of a few square centimeters. The characteristics and advantages of flexible combination and large-scale integration on the small platform controlled by the system. Microfluidic chips have the advantages of saving space and reagents, small heat capacity, fast heating and cooling rates, and easy integration. The main feature of a microfluidic chip on a device is that its effective structure for containing fluids (including channels, reaction chambers, and certain other functional components) is micron-scale in at least one dimension....

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/38C12M1/34
CPCB01L7/52B01L2300/0627B01L2300/0819B01L2300/1805
Inventor 赵树弥朱灵邓国庆王贻坤朱灿灿张龙李志刚刘勇王安
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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