Multifunctional integrated microfluidic chip for conducting online screening of multiple types of cancer cells simultaneously

A technology of microfluidic chips and tumor cells, applied in the field of multifunctional integrated microfluidic chips, can solve problems such as inability to break through, achieve the effect of reducing experimental errors and saving time

Active Publication Date: 2016-08-10
浙江弘瑞医疗科技有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

However, as far as the current technological development is concerned, the screening of multiple functions on one chip is still the bottleneck of high-throughput screening of drugs, which has become a major problem and cannot be broken through for a long time.

Method used

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  • Multifunctional integrated microfluidic chip for conducting online screening of multiple types of cancer cells simultaneously
  • Multifunctional integrated microfluidic chip for conducting online screening of multiple types of cancer cells simultaneously
  • Multifunctional integrated microfluidic chip for conducting online screening of multiple types of cancer cells simultaneously

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Embodiment Construction

[0040] The present invention will be further described below in conjunction with the drawings.

[0041] Such as figure 1 As shown, the microfluidic chip includes from top to bottom: a first PDMS film layer, a second PDMS film layer, and a glass bottom layer. The three parts are successively irreversibly plasma bonded to form a composite integrated structure.

[0042] 24 microvalves are integrated on the first PDMS film layer, including 16 air valves and 8 liquid valves; the air valves are used in pairs in two groups, a total of 8 groups, each group includes two air valves, respectively Is the first air valve a of the first group, the second air valve a1 of the first group, the first air valve b1 of the second group, the second air valve b of the second group; the liquid valves are matched in pairs Used, a total of four groups, each group includes two liquid valves; the first liquid valve A and the second liquid valve B.

[0043] The second PDMS film layer is a fluid channel layer. F...

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Abstract

The invention provides a multifunctional integrated microfluidic chip for conducting online screening of multiple types of cancer cells simultaneously and belongs to the technical field of microfluidics. By means of the multifunctional integrated microfluidic chip, the experiment of conducting online screening of multiple types of cancer cells simultaneously can be completed, and 16 types of cancer cells can be inoculated; online screening of multiple types of medicine simultaneously can be achieved, and online screening of eight types of cancer-resistant medicine simultaneously can be completed. The multifunctional integrated microfluidic chip comprises three layers, namely a first PDMS film layer, a second PDMS film layer and a glass bottom layer, and the three parts are bonded by irreversible plasms from top to bottom in sequence to form a composite integrated structure. Four modules of the same structure and a waste liquid opening are integrated into a chip body, four columns of cell culture channels, a fluid channel, a cell injection opening, two medicine injection openings and six micro valves are integrated into each module, a bent pipe communicated with the cell culture channels is arranged below each column of cell culture channels, and the top end of each bent pipe is provided with an outlet.

Description

Technical field [0001] The invention belongs to the technical field of microfluidics, and specifically relates to a multifunctional integrated microfluidic chip for simultaneous online screening of multiple tumor cells. Background technique [0002] Drug screening is the initial process and key step of new drug research and the only way to discover new drugs. However, when the traditional 96-well plate technology is applied in the research and development of new Chinese medicines, due to its shortcomings such as large reagent consumption, cumbersome manual operations, and large human experiment errors, it causes bottlenecks such as long new drug development cycles, high costs, and high risk of late failure. To a large extent, it limits the screening and development of modern drugs. At present, the concept of miniaturized total analysis systems (μTAS) has been put forward, with its advantages of high throughput, high sensitivity, and high efficiency, and has been widely used in l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/34C12Q1/02
CPCB01L3/502707B01L2300/0819B01L2300/0861G01N33/5011
Inventor 孟宪生樊佳新包永睿王帅李天娇
Owner 浙江弘瑞医疗科技有限公司
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