Single-cell-sorting and multigene-locus-detection micro-fluidic chip for expressing EGFR

Abstract

The invention provides a single-cell-sorting and multigene-locus-detection micro-fluidic chip for expressing EGFR. The micro-fluidic chip comprises a top cover, a micro-fluid channel layer, a substrate and a base plate which are sequentially stacked from top to bottom and sealed with one another, wherein the substrate is provided with micro-pore arrays and the base plate is provided with cavity groups. The invention further provides a system matched with the chip and used for detecting the single cell level of multiple mutation sites of EGFR genes. The micro-fluidic chip and the corresponding detecting system can screen out target cells from a large amount of cells fast, simply and cheaply and perform EGFR multi-gene-site analysis on the single cell in the target cells.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a microfluidic chip suitable for single-cell sorting and multi-gene site detection for expressing EGFR. Background technique [0002] In recent years, the morbidity and mortality of lung cancer have risen rapidly, becoming the number one cancer in China. Although the treatment technology has been greatly improved, the 5-year survival rate has not improved significantly. Among lung cancers, non-small cell lung cancer (NSCLC) is the most representative, accounting for about 80%. At present, targeted therapy against EGFR has become a research hotspot, and has achieved good curative effect. EGFR (Epidermal Growth Factor Receptor) is a receptor for epidermal growth factor (EGF) cell proliferation and signal transduction. EGFR belongs to the ErbB receptor family, which includes EGFR (ErbB-1), HER2 / c-neu (ErbB-2), Her3 (ErbB-3) and Her 4 (ErbB-4). It has been confirmed tha...

AI Technical Summary

Problems solved by technology

However, based on thermal cycling and PCR (polymerase chain reaction)-based whole-genome amplification technology, in the PCR process, when the initial sample is very rare, many errors and non-specific amplification products will be introduced into the sequence, making amplification difficult. The amplification quality is greatly reduced, and only a single gene locus can be checked by specific primers at a time, and multi-locus gene amplification cannot be performed on the same sample

[0006] None of the currently disclosed technologies involve highly integrated screening, identification, lysis, amplification and multi-gene site analysis of EGFR tumor cells, so as to realize multi-site EGFR gene mutation detection on tumor cells at the single-cell level

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