Single-cell-sorting and multigene-locus-detection micro-fluidic chip for expressing EGFR

A microfluidic chip and single-cell technology, applied in animal cells, fluid controllers, tumor/cancer cells, etc., can solve the problem of screening, identifying, lysing, and reducing the quality of amplification of tumor cells that do not involve highly integrated EGFR. Problems such as locus gene amplification, to achieve the effect of easy loss and loss, reducing loss and reducing impact

Pending Publication Date: 2016-11-23
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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Problems solved by technology

However, based on thermal cycling and PCR (polymerase chain reaction)-based whole-genome amplification technology, in the PCR process, when the initial sample is very rare, many errors and non-specific amplification products will be introduced into the sequence, making amplification difficult. The amplification quality is greatly reduced, and only a single gene locus can be che

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  • Single-cell-sorting and multigene-locus-detection micro-fluidic chip for expressing EGFR
  • Single-cell-sorting and multigene-locus-detection micro-fluidic chip for expressing EGFR
  • Single-cell-sorting and multigene-locus-detection micro-fluidic chip for expressing EGFR

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Example Embodiment

[0038] Example 1 Microfluidic chip for single cell sorting and multi-gene locus detection expressing EGFR and preparation method thereof

[0039] The microfluidic chip provided in this embodiment includes a four-layer structure stacked one after another and sealed to each other. From top to bottom, there are a top cover, a microfluidic channel layer, a substrate with a microporous array, and a cavity group. Of the bottom plate.

[0040] 1. Top cover and fluid passage

[0041] When identifying cells, light needs to pass through the top cover, so the top cover needs to be made of transparent material; at the same time, the top cover needs to be made with fluid inlet and outflow interfaces, so it needs to be able to open holes; finally, the top cover needs to be sealed with the fluid channel They are connected together and sealed, so the material selection should consider the compatibility with the fluid channel.

[0042] The substrate material can be made into a material with a specifi...

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Abstract

The present invention provides a microfluidic chip for sorting single cells expressing EGFR and detecting multiple gene loci. The microfluidic chip includes a four-layer structure stacked together and sealed with each other, from top to bottom They are respectively a top cover, a microfluidic channel layer, a substrate with a microhole array and a bottom plate with a cavity group. The invention also provides a system for single-cell level detection of multiple mutation sites of EGFR gene matched with the chip. The microfluidic chip and the corresponding detection system of the present invention can quickly, easily and cheaply sort target cells from a large number of cells, and perform EGFR multi-gene site analysis on a single cell among them.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a microfluidic chip suitable for single-cell sorting and multi-gene site detection for expressing EGFR. Background technique [0002] In recent years, the morbidity and mortality of lung cancer have risen rapidly, becoming the number one cancer in China. Although the treatment technology has been greatly improved, the 5-year survival rate has not improved significantly. Among lung cancers, non-small cell lung cancer (NSCLC) is the most representative, accounting for about 80%. At present, targeted therapy against EGFR has become a research hotspot, and has achieved good curative effect. EGFR (Epidermal Growth Factor Receptor) is a receptor for epidermal growth factor (EGF) cell proliferation and signal transduction. EGFR belongs to the ErbB receptor family, which includes EGFR (ErbB-1), HER2 / c-neu (ErbB-2), Her3 (ErbB-3) and Her 4 (ErbB-4). It has been confirmed tha...

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Application Information

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IPC IPC(8): C12M1/42C12M1/36C12M1/34C12M1/12C12M1/00C12N5/09C12Q1/68G01N33/573
CPCG01N33/573C12N5/0693C12Q1/6886B01L3/502753B01L2200/10B01L2200/0647B01L2400/0415B01L2300/0832B01L2300/0681B01L2300/0864B01L2300/088B01L2300/0887G01N2333/9121C12Q2600/156
Inventor 李仁周明行胡志远魏泽文
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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