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Preparing method for Alzheimer disease animal model

A technology of Alzheimer's disease and animal models, applied in animal husbandry, etc., can solve problems such as low success rate, high cost, complex technology, etc., and achieve high success rate, stable properties, and clear mechanism

Inactive Publication Date: 2004-11-17
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although senile plaques, the pathological changes unique to AD, appeared in the brains of transgenic animal models, most AD patients were sporadic without these gene mutations, and less than 10% of AD patients had familial, and most of them did not. Gene mutations can be found, so transgenic animal models cannot fully represent human AD. In addition, the establishment of transgenic animal models requires complex techniques and high costs, and there are still great limitations in popularization and application.
[0004] These two models have the following defects: 1. In the existing non-transgenic AD models, there have only been reports of increased Aβ-like immunopositive neurons (APLI) and no senile plaque characteristics
Cannot yet have all the main features of AD
2. Although the transgenic animal model has the pathological characteristics of senile plaques, AD patients with genetic characteristics only account for 1% of all AD patients, and the success rate is low and the cost is expensive

Method used

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  • Preparing method for Alzheimer disease animal model
  • Preparing method for Alzheimer disease animal model
  • Preparing method for Alzheimer disease animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The Kunming mice were adaptively fed for one week, and the animals were randomly divided into two groups, 20 in each group, the blank control group and the AD model group. Intraperitoneal injection of D-galactose 60mg·kg in the model group and the administration group -1 d -1 (Preparation of normal saline) and gavage of aluminum trichloride 5mg·kg -1 d -1 (double distilled water configuration), for 150 consecutive days, the blank control group was intraperitoneally injected with an equal amount of normal saline and gavaged with an equivalent amount of double distilled water.

[0021] Model test results:

[0022] 1. The decline of learning and memory ability

[0023] After modeling, the learning and memory ability of AD model mice was tested by Norris water maze.

[0024] 1. Escape latency of mice in each group

[0025] Table 1 Morris water maze escape latency of mice in each group (x±s, n=20) unit: s)

[0026] Groups Day 1 Day 2 Day 3 Day 4 Day 5

[00...

Embodiment 2

[0065] The Kunming mice were adaptively fed for one week, and the animals were randomly divided into two groups, 20 in each group, the blank control group and the AD model group. Intraperitoneal injection of D-galactose 180mg·kg in the model group and the administration group -1 d -1 (prepared with normal saline) and gavage aluminum trichloride 50mg·kg -1 d -1 (double distilled water configuration), for 90 consecutive days, the blank control group was intraperitoneally injected with an equal amount of normal saline and gavaged with an equivalent amount of double distilled water.

[0066] Model test results:

[0067] 1. The decline of learning and memory ability

[0068] After modeling, the learning and memory ability of AD model mice was detected by Morris water maze.

[0069] 1. Escape latency of mice in each group

[0070] Table 6 Morris water maze escape latency of mice in each group (x±s, n=20) unit: s)

[0071] Groups Day 1 Day 2 Day 3 Day 4 Day 5

...

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Abstract

The invention relates to a method for preparing Alzheimer animal model which comprises, using mouse, rat or other mammal, administering D-galactose through abdominal cavity injection, affording aluminium chloride (AlCl3) through stomach filling simultaneously, after a finite period of time, the animal learning and memorizing function decreases, the brain generating the characteristic pathological alteration, i.e. senile plaque (SP), neurofi brillary tangle (NFT) and different degree of loss for cholinergic neuron and other nerve cells.

Description

technical field [0001] The invention relates to the technical field of medical evaluation and detection, in particular to a preparation method of an animal model for screening and treating Alzheimer's disease drugs and evaluating methods for treating Alzheimer's disease. Background technique [0002] Preparation of Alzheimer's disease (Alzheimer's referred to as AD, also known as senile dementia, the same below) animal model can be carried out by carrying out various experiments on the animal model, and its pathological morphology can be compared and analyzed, and its counting data and measurement data Statistical processing and analysis can also be carried out, so as to facilitate the research on the pathogenesis and treatment methods (including therapeutic drugs) of the disease. [0003] The preparation methods of existing senile dementia disease animal models include: 1, non-transgenic AD model, including physical (operation, electricity, heat, etc.) cholinergic neuron d...

Claims

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Application Information

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IPC IPC(8): A01K67/027
Inventor 罗焕敏肖飞
Owner JINAN UNIVERSITY
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