Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Three-dimensional combined micro-channel for inducing orientated growth of primary cortical neuron axons

A technology of directional growth and neurons, applied in methods of supporting/immobilizing microorganisms, specific-purpose bioreactors/fermenters, biochemical instruments, etc. Oriented growth of axons in cortical neurons

Active Publication Date: 2022-03-11
BEIJING INSTITUTE OF TECHNOLOGYGY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The main purpose of this application is to provide a three-dimensional combined microfluidic channel that induces the directional growth of the axons of primary cortical neurons, so as to solve the problem that it is difficult to realize the three-dimensional directional growth of nerve axons under the condition of directional flow in the related art, which makes it difficult Issues in studying axon-directed outgrowth of primary cortical neurons in tissue models

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Three-dimensional combined micro-channel for inducing orientated growth of primary cortical neuron axons
  • Three-dimensional combined micro-channel for inducing orientated growth of primary cortical neuron axons
  • Three-dimensional combined micro-channel for inducing orientated growth of primary cortical neuron axons

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiment of the application will be clearly and completely described below in conjunction with the accompanying drawings in the embodiment of the application. Obviously, the described embodiment is only It is an embodiment of a part of the application, but not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without creative efforts shall fall within the scope of protection of this application.

[0030] It should be noted that the terms "first" and "second" in the description and claims of the present application and the above drawings are used to distinguish similar objects, but not necessarily used to describe a specific sequence or sequence. It should be understood that the data so used may be interchanged under appropriate circumstances for...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a three-dimensional combined micro-channel for inducing orientated growth of primary cortical neuron axons. The three-dimensional combined micro-channel comprises a soft photoetching supporting channel and a two-photon photoetching axon growth bracket, a first interaction interface and a second interaction interface are formed in the soft lithography supporting flow channel, and a first cell chamber and a second cell chamber are arranged in the soft lithography supporting flow channel; the first cell chamber is communicated with the first interaction interface, and the second cell chamber is communicated with the second interaction interface; the two-photon photoetching axon growth support is embedded in the soft photoetching supporting flow channel, the two-photon photoetching axon growth support comprises a growth cavity penetrating through the two-photon photoetching axon growth support, the first end of the growth cavity is communicated with the first interaction interface, and the second end of the growth cavity is communicated with the second interaction interface; the growth cavity comprises at least one ascending section, and the ascending section is formed by changing the growth cavity in the Z-axis direction. The problem that three-dimensional directional growth of nerve axons under the directional flowing condition is difficult to achieve through a flow channel in the prior art is solved.

Description

technical field [0001] The present application relates to the technical field of cell culture, in particular to a three-dimensional combined microfluidic channel for inducing the directional growth of primary cortical neuron axons. Background technique [0002] Three-dimensional artificial microfluidics that can induce the directional growth of axons in primary cortical neurons are indispensable and important neural tissue models in the research of human neurodegenerative diseases and clinical medical tests. Directed growth of axons can be used for damage testing and secretion analysis, which has great guiding significance for the study of irreversible diseases such as Alzheimer's disease, Parkinson's disease and Huntington's disease. [0003] The existing artificial microfluidics that can induce the directional growth of primary cortical neuron axons are mainly two-dimensional microfluidics, while the only three-dimensional microfluidics only include "3D" gel-filled two-dim...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
CPCC12M23/16C12M25/14C12M21/08
Inventor 王化平刘梦华董欣怡石青刘佳欣黄强福田敏男
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com