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A tissue engineered neural tissue and its construction method

A nerve tissue and tissue engineering technology, applied in biochemical equipment and methods, tissue culture, nervous system cells, etc., can solve problems such as inappropriate mechanical properties and weak texture, and achieve easy repeatability, simple operation steps, and rich network connections Effect

Active Publication Date: 2020-10-13
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent with the publication number CN102228718A discloses a tissue-engineered nerve tissue and its construction method. The collagen / Matrigel glue used in this technical solution is a hydrogel biomaterial with a weaker texture and better mechanical properties than nerve cells. Not suitable, but also easy to degrade

Method used

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  • A tissue engineered neural tissue and its construction method
  • A tissue engineered neural tissue and its construction method
  • A tissue engineered neural tissue and its construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Extracting silk fibroin solution: Cut silkworm cocoons into pieces about the size of a fingernail. Prepare 2L Na 2 CO 3 solution (4.24g of sodium carbonate dissolved in 2L of water) was boiled, 5g of silkworm cocoon fragments were weighed, boiled in the sodium carbonate solution for 30 minutes, and continuously stirred with a stirring rod to remove sericin. Take out the remaining silk fibroin, and wash it repeatedly with distilled water for at least three times, so as to fully remove the remaining silk fibroin and other impurities. The silk fibroin was wrung out and dried overnight in a fume hood. The next day, put the silk fibroin solution in 9.3M LiBr solution at 60°C for 4 hours. Prepare a dialysis bag for MWCO3500, use a syringe to fill the solution silk fibroin solution into the dialysis bag, use distilled water for dialysis for 48 hours, and change the water every few hours. Put the silk fibroin solution in the dialysis bag into a centrifuge tube, centrifu...

Embodiment 2

[0039] 1. Scanning electron microscope observation and mechanical test

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Abstract

The invention discloses tissue engineering neural tissue and a constructing method thereof, and belongs to the technical field of tissue engineering. Silk fibroin and collagen composite materials areadopted, primary cortical neurons are adopted as seed cells for three-dimensional culture, and tissue engineering neural tissue is formed. The operation steps are simple, and can be easily executed repeatedly, and no special technology or equipment requirement is met. The neural tissue constructed under the system generates a neural network with abundant network connection. The mechanical performance of the adopted silk fibroin is suitable for neural cell inoculation and culture, and the elasticity modulus of the neural tissue is close to that of rat brain tissue.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a tissue-engineered nerve tissue and a construction method thereof. Background technique [0002] The human brain is the most advanced part of the nervous system, the largest and most complex structure in the central nervous system, the organ that regulates body functions, and the material basis for advanced neural activities such as consciousness, spirit, language, learning and memory. The structure of the human brain is complex, with abundant folds, complex neural networks and synaptic connections. In order to gain insight into the basic units of the brain, such as the formation and activity of neural networks, in vitro brain research models can be used to recapitulate the complexity of natural brain tissue structure and function. In vitro brain models are designed to reconstruct the basic structure of brain components and to conduct cell biology studies...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0793C07K14/435
CPCC07K14/43586C12N5/0619C12N2533/50C12N2533/54
Inventor 王常勇朱惠敏周瑾刘伟王春兰
Owner ACADEMY OF MILITARY MEDICAL SCI
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