Method for screening myocardial ischemia resisting pharmacodynamic material basis of yang exhaustion treating decoction

Abstract

The invention discloses a method for screening myocardial ischemia resisting pharmacodynamic material basis of yang exhaustion treating decoction. The method comprises the following steps: 1, determining myocardial ischemia resisting endogenous metabolin of the yang exhaustion treating decoction and measuring the peak area; 2, determining in-vivo migration constituents in the yang exhaustion treating decoction and measuring the peak area; 3, performing correlation analysis on the peak area of the myocardial ischemia resisting endogenous metabolin of the yang exhaustion treating decoction and the peak area of the in-vivo migration constituents in the yang exhaustion treating decoction and screening out myocardial ischemia resisting pharmacodynamic material basis of the yang exhaustion treating decoction. The method disclosed by the invention is based on isoproterenol-induced rat myocardial ischemia model; an ultrahigh performance liquid chromatography-quadrupole rod-flight time mass spectrum non-targeted metabonomic technology and a targeted metabonomic technology based on an ultrahigh performance liquid chromatography-triple quadrupole rod mass spectrum are utilized to screen out 12 kinds of myocardial ischemia resisting pharmacodynamic material basis of the yang exhaustion treating decoction; thus, accuracy of the myocardial ischemia resisting pharmacodynamic material basis ofthe yang exhaustion treating decoction is improved, a screening period is shortened, and screening consumption cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for screening the material basis of anti-myocardial ischemia efficacy of Sini decoction. Background technique [0002] Sini Decoction is composed of aconite, dried ginger, and licorice. It is a classic prescription in "Treatise on Febrile Diseases" written by Zhang Zhongjing. . Sini Decoction has been valued by physicians of all dynasties because of its strong medicinal power and outstanding curative effect. Studies have found that aconitum alkaloids in aconite, gingerol compounds and total volatile oil in dried ginger, and flavonoids and saponins in licorice are The main chemical components in Sini soup. However, what are the real medicinal ingredients of Sini Decoction to exert cardiovascular activity? What is the interaction relationship between these medicinal ingredients and biological activity? These are the basis for further research and developme...

AI Technical Summary

Problems solved by technology

However, this method has a long cycle and low throughput, and it decomposes and ignores the essence of the overall effect of "multi-component-multi-target" of traditional Chinese medicine

However, the multi-component "spectrum-effect" research of traditional Chinese medicine is mostly limited to the "system-point" research between the "multi-component spectrum" and the "single effect" index of traditional Chinese medicine, which still cannot reflect the influence of traditional Chinese medicine through multiple components and multiple targets. Synergistic features

In addition, due to the diversity of traditional Chinese medicine effects, there are also problems in the scientificity and rationality of the selection of drug efficacy indicators.

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