Primer combination sequence and kit for detecting child safe medication-related gene mutation sites

Abstract

The invention discloses a primer combination sequence and kit for detecting child safe medication-related gene mutation sites. The kit includes 23 pairs of amplification primers and 23 single-base extension primers; the 23 pairs of amplification primers can specifically amplify the 23 common mutation site regions of child safe medication-related genes, and the 23 single-base extension primers areused to detect the 23 mutation site genotypes of the child safe medication-related genes; and the kit further includes special reagents for pretreatment and detection. The kit provided by the invention can realize one-hole detection of the 23 mutations related to the clinical child safe medication-related genes, has high sensitivity, strong specificity and high accuracy, and is simple to operate,low in cost and high in throughput, the detection is fast, automatic interpretation of results is realized, and the clinical promotion and application are easy to realize; and the kit can be applied to the detection of the genes related to safe medication of newborns or children, guide clinical correct medication, avoid medication risks, reduce medication injury, and truly achieve precise medication.

Description

technical field [0001] The present invention relates to the technical field of molecular biological detection, more specifically, relates to the use of multiplex PCR technology in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF) technology, and the use of mass spectrometry technology in combination with multi-primer extension technology and MassARRAY technology for safe drug use for children Primer sequences and kits for genetic testing. [0002] technical background [0003] At present, drug poisoning in children has become a global public health problem, and 125 children die from poisoning every day in the world. Poisoning is also one of the leading causes of child injury death in China. According to the data in the State Food and Drug Administration's "2016 Pediatric Medication Safety Investigation Report White Paper", among children, the proportion of drug poisoning in all poisoned children increased from 53% in 20...

AI Technical Summary

Problems solved by technology

[0005] There are more than 3,500 kinds of pharmaceutical preparations in my country, and only more than 60 kinds are specially used for children, accounting for less than 2%. Drugs do not have specific dosage forms suitable for children; adult drugs are commonly used in children, but children are not smaller versions of adults

Fluorescence quantification combined with melting curve method has limited single-well detection throughput, and can only detect known mutations at a small number of sites. Specific primers need to be designed for each mutant genotype at each site; multi-hole and multiplex per well is required to cover common sites. Detection, multiple probe design requirements are high, the technology is difficult, the cost of detection probes is high, and the results are interpreted manually, which is prone to misjudgments; the reverse dot hybridization system needs to design probes for each locus and each genotype, and the cost High, the resolution depends on the effectiveness of the probe, it is prone to non-specificity, and the detection process is long, the operation is complicated, the system is open, and the samples are easily contaminated; the Sanger sequencing method is the gold standard for sequencing, but the process is long, the operation is complicated, and requires Manual interpretation of each locus, covering common loci requires multi-hole detection, high cost, and low throughput; although high-throughput sequencing can detect all relevant loci covering children’s safe medication genes at one time, the cost of instruments and testing reagents High, when the number of test samples is small, it will cause waste, resulting in higher costs, and the system requires a high amount of nucleic acid samples (above 50ng), and newborn heel blood samples are small and low in concentration, often requiring screening of multiple items Therefore, it is difficult to obtain enough nucleic acid templates; the high-throughput sequencing detection process lasts for 3-5 days, the operation is complicated, and professional bioinformatics analysts are required to analyze the data, and the result interpretation is difficult, so it cannot be used as a screening item in the Large-scale promotion and application of local small and medium-sized hospitals at all levels across the country

Images