41results about How to "Analysis of results is simple" patented technology

The invention discloses a primer combination sequence and kit for detecting child safe medication-related gene mutation sites. The kit includes 23 pairs of amplification primers and 23 single-base extension primers; the 23 pairs of amplification primers can specifically amplify the 23 common mutation site regions of child safe medication-related genes, and the 23 single-base extension primers areused to detect the 23 mutation site genotypes of the child safe medication-related genes; and the kit further includes special reagents for pretreatment and detection. The kit provided by the invention can realize one-hole detection of the 23 mutations related to the clinical child safe medication-related genes, has high sensitivity, strong specificity and high accuracy, and is simple to operate,low in cost and high in throughput, the detection is fast, automatic interpretation of results is realized, and the clinical promotion and application are easy to realize; and the kit can be applied to the detection of the genes related to safe medication of newborns or children, guide clinical correct medication, avoid medication risks, reduce medication injury, and truly achieve precise medication.

The invention relates to a kit for detecting human PEAR1 gene polymorphism and an application thereof. The kit includes the following substances: a specific primer and a specific fluorescent probe for detecting an rs2768759 locus on PEAR1 gene, a specific primer and a specific fluorescent probe for detecting an rs12041331 locus on the PEAR1 gene, a Taq DNA polymerase, dNTP mixed liquid, a MgCl2 solution, a fluorescent quantitative PCR reaction buffer liquid and deionized water. The kit overcomes the defects in various gene mutation detection methods, is high in detection accuracy, is simple and convenient, is short in detection time and is simple in result analysis. The kit is suitable for clinical laboratories and can perform qualitative detection to the two polymorphic sites, the rs2768759 and the rs12041331, on the PEAR1 gene, wherein a PCR fluorescent amplification reaction is carried out according to the SNP locus. A result can be determined just on the basis of whether two different fluorescent curves are positive or not without manual error, so that the kit is improved in efficiency and reduced in detection cost. The kit is free of DNA extraction, wherein a suspension liquid after cell pyrolysis is added to the reaction system to complete amplification.

The invention discloses a primer sequence and kit for detecting pathogenic mutation related to glucose-6-phosphate dehydrogenase (G6PD) deficiency. The kit comprises five pairs of amplification primers, 16 single-base extension primers and a special reagent for pretreatment and detection, wherein the amplification primers can specifically amplify 16 common mutation site regions of G6PD genes related to G6PD deficiency; and the 16 single-base extension primers are used for detecting genotypes of 16 mutation sites of the G6PD genes. By the kit, one-hole detection of 16 common mutations related to clinical G6PD deficiency pathopoiesis can be realized; and the kit is high in sensitivity, specificity and accuracy, simple and convenient to operate, low in cost, high in throughput, quick in detection, automatic in result interpretation and easy for clinical popularization and application. The primer sequence and kit can be applied to prenatal diagnosis or neonatal hereditary disease screeningdetection, and a reliable detection reagent is provided for quick detection of G6PD deficiency carriers or patients.

The invention discloses a method for monitoring foul and damp degree on the surface of an insulator, which comprises the following steps: S1, in a measuring circuit, collecting the voltage signals of a fixed resistor and a power supply; S2, subtracting the initial phase angles of the two voltage signals, and taking the absolute value of the difference value to obtain phase angle difference theta; S3, obtaining the relational expression between the phase angle difference theta and the equivalent resistance Rx of foulness on the surface of the insulator; S4, according to the change of the phase angle difference, obtaining the foul and damp degree on the surface of the insulator. According to the invention, the measurement error is less, the equipment is cheap , and the analysis of the monitoring result is simple.

The invention relates to a kit for detecting human ABCB1 gene polymorphism and application thereof. The materials in the box of the present invention include specific primers and specific fluorescent probes for detecting the SNP site rs1045642 on the ABCB1 gene, Taq DNA polymerase, dNTP mixture, MgCl 2 solution, fluorescent quantitative PCR reaction buffer and deionized water. The invention overcomes the respective defects of various gene mutation detection methods. The invention has the advantages of high detection accuracy, simple and convenient detection, short detection time, and simple result analysis, and is suitable for use in clinical laboratories. It can perform qualitative detection of CYP2C19 gene polymorphism sites, and perform PCR fluorescence amplification reactions according to SNP sites. , the results can be judged only according to whether the two different fluorescence curves are on the line, there will be no human error, the false positive and false negative rates are low, the detection cost is reduced, no DNA extraction is required, and the expansion can be completed only by lysing whole blood cells increase to improve detection accuracy.

The invention relates to a dual polymerase chain reaction-denaturing high performance liquid chromatography (PCR-DHPLC) detection method for staphylococcus aureus in aquatic products, which is characterized by comprising the following steps of: 1, staphylococcus aureus culture: aquatic product samples to be tested are mixed with enrichment liquid for culture; 2, staphylococcus aureus genome deoxyribonucleic acid (DNA) extraction; 3, primer design; 4, dual PCR amplification by using the staphylococcus aureus genome DNA as templates; and 5, DHPLC peak type map obtaining through PCR product DHPLC detection for analysis and identification. The integral detection method has the advantages that the reaction condition is simplified, the detection sensitivity is improved, in addition, the operation is simpler and more convenient, and the goal of high flux is reached.

The invention relates to the field of molecular genetic breeding and particularly provides a method for rapidly detecting LOX (lipoxygenase) transgenic wheat and a kit using the method. Specific and sensitive outer primers including F3 and B3 and inner primers including FIP and BIP are taken as amplification target sequences. The principle of the detection method is as follows: if the color of a reaction liquid turns from purplish blue to sky blue through DNA (deoxyribonucleic acid) extraction and LAMP (loop-mediated isothermal amplification) detection, detected wheat belongs to a transgenic wheat strain. The method has low requirement for the quality of a DNA template, the LAMP detection cycle is short, the detection cost is low, the operation process is simple and convenient, and a result is easy to analyze.

The invention belongs to the field of gene diagnosis, and discloses a real-time fluorescence PCR method of detecting a rs762551 site of a CYP1A2 gene, and a primer-and-probe combination of the real-time fluorescence PCR method. A sequence of one forward primer FpG is 5'-GGTGAGCTCTGTGGGGC-3', a sequence of the other forward primer FpG is 5'-GGTGAGCTCTGTGGGGA-3', a sequence of a reverse primer Rp is5'-GCTGAGGGTTGAGATGGAGAC-3', and a sequence of a probe Probe is 5'-Cy5-ACGCATGGTAGATGGAGCTTAG-BHQ2-3'. After a PCR is completed, results can be analyzed through whether or not an amplification curveexists and in combination with a Ct value. The real-time fluorescence PCR method of detecting the rs762551 site of the CYP1A2 gene is applied to polymorphism detection of the rs762551 site, and has the advantages of being easy to operate, high in resolution, free of pollution, and high in throughput.

The invention relates to a decidua and villus pairing cDNA chip. The cDNA chip is prepared through the following method that the decidua and villus tissue of a woman with early pregnancy abortion are taken, the RNA is extracted, cDNA is formed by reverse transcription and preset in a PCR reaction plate, and cDNA chip is made. A preparation method and application of cDNA chip are further provided. The method and application have the advantages that the decidua and villus tissue of every abortion woman are respectively taken, pairing is formed by cDNA, the cDNA pairs are concentrated on the same chip, and the function presentation of parent-fetal interface of the physiological structure is achieved on the chip outside the body. The decidua and villus pairing cDNA chip has the advantages of being high in throughput, strong in specificity, high in sensitivity, simple and fast in operation, high in quantitative accuracy, strong in result reliability, simple in result analysis, complete in clinical information for samples and the like, and the cDNA chip can be used for quantitative detection of the expression statuses of a target gene in different patients and for conducting in-depth correlation analyses of clinical information.

The invention relates to a fluorescent polarization based homogeneous phase detection method of a single nucleotide polymorphism of a codon118 of an ERCC1 (excision repair cross-complementing 1) gene. The homogeneous phase detection method solves the problems of high cost, complicated operating steps, proneness to causing contamination and influence on detection result accuracy in the prior art, and is simple in steps, easy to operate, not prone to causing contamination as operation can be completed once in a closed tube, and low in cost as any special reagent and fluorescence quenching or micro-groove binding agent are not needed. Application of a specific probe with single end marked to detection of the single nucleotide polymorphism of the codon118 of the ERCC1 gene results in changes of fluorescent polarization values and enables detection results to be more objective and accurate. Result analysis is simple as only numeric comparison is needed in result judgment, and the homogeneous phase detection method can easily realize standardization and automation, is wide in application range and can be used for detecting clinical blood or tissue specimens.

The invention relates to a kit for detecting human COX-2 gene polymorphism and an application thereof. The kit includes the following substances: a specific primer and a specific fluorescent probe for detecting an rs20417 SNP locus on COX-2 gene, a Taq DNA polymerase, a dNTP mixed liquid, a MgCl2 solution, a fluorescent quantitative PCR reaction buffer liquid and deionized water. The kit overcomes the defects in various gene mutation detection methods, is high in detection accuracy, is simple and convenient, is short in detection time and is simple in result analysis. The kit is suitable for clinical laboratories and can perform qualitative detection to COX-2 gene polymorphic sites, wherein a PCR fluorescent amplification reaction is carried out according to the SNP locus. A result can be determined just on the basis of whether two different fluorescent curves are positive or not, so that the kit is free of manual error, is low in false positive and false negative rate, and is improved in efficiency and reduced in detection cost.

The invention belongs to the field of gene diagnosis, and discloses a TaqMan probe real-time fluorescent PCR method for detecting the rs6313 locus (HTR2A, 102C>T) of a HTR2A gene, and a primer probe combination. A upstream primer FpC-T is 5'-GCTCTACAGTAATGACTTTAACTTC-3', a upstream primer FpT-T is 5'-GCTCTACAGTAATGACTTTAACTTT-3', a downstream primer Rp is 5'-GATGAAGTAAGGAGAGACACGAC-3', and a probeProbe is 5'-FAM-TAACACTTCTGATGCATTTAACTGGAC-BHQ2-3'. After PCR reaction is completed, the presence or absence of an amplification curve can be used for analyzing the result. The TaqMan probe real-time fluorescent PCR method has the advantages of simple operation, high resolution, no pollution, high throughput and the like.

The invention relates to a homogeneous phase detection method for a methylation state of an epidermal growth factor receptor (EGFR) gene promoter based on fluorescence polarization. The method solves the problems that the cost is high, the operation steps are fussy, pollution is easily produced and the accuracy of the detection result is affected in the prior art; the method is simple and easy to operate; and the method is finished by one step in a closed tube, and does not produce pollution. The method is low in cost, and does not require any special reagent and fluorescence quenching or micro groove bonding agent. Methylation of a target region is detected by using change of a fluorescence polarization value due to a single end-marked methylated specific probe, so that the detection result is objective and accurate. The method is simple in result analysis; and only digital comparison is required during result judgment, so the method easily realizes standardization and automation, has a wide application range and can be used for detecting clinical blood or tissue specimens.

The invention discloses a detection method of a hypertriglyceridemia mutant site. Through the design of a specific primer, a target fragment is amplified by single-tube multiplex PCR (polymerase chainreaction); a sequencing primer is designed; a non-glue-cutting purified Sanger sequencing method is used for performing sequencing on the obtained target fragment. The invention also discloses a detection kit of the hypertriglyceridemia mutant site. The multiplex amplification LPL (lipoprotein lipase) isogene and the non-glue-cutting purification sequencing can be realized; only the target gene is amplified; the advantages of low cost and exact target goal are realized. Dozens of fragments are amplified at the same time through a single tube; the detection flux is high; the problem of time consumption and labor waste of the single PCR are solved; the operation is simple and fast; the operation can be performed by ordinary experiment personnel according to the description; during the sequencing, the glue cutting purification is not needed; the result shows that the operation is convenient; the PCR multiple number is sufficient; the identical or similar band strip size does not need tobe worried. The popularization is easy; the market prospects are wide.

The invention belongs to the technical field of detection of environmental microorganisms by a biological method, and discloses a marine phaeocystis globosa single-cell detection method based on a TaqMan probe technology. The method comprises the following steps: S1, designing and synthesizing a primer and a probe; S2, preparing a positive control sample of the phaeocystis globosa; S3, performingnegative control quality control; S4, extracting genomic DNA of a marine single-cell phaeocystis globosa sample and a positive control sample; S5, performing fluorescent quantitative PCR detection byadopting the primer and the probe designed in the S1; S6, judging a real-time fluorescent quantitative PCR amplification signal; S7, carrying out agarose gel electrophoresis analysis on a fluorescentquantitative PCR product; S8, carrying out sequencing analysis on a fluorescent quantitative PCR product; and S9, detecting and analyzing marine single-cell phaeocystis globosa. According to the method, low-density harmful red tide algae in the early stage of harmful red tide outbreak can be detected, so that an effective early warning tool is provided for early warning and early prevention and control of red tide outbreak, and the economic loss of mass culture and tourism nuclear power industries is avoided.

The invention discloses a primer group, a method and a kit for detecting SLCO1B1 and APOE gene polymorphism based on a shared primer probe. According to the method, reporter groups are marked at 5'ends of wild ARMS primers and mutant ARMS primers which are designed in the same direction to prepare ARMS primers with probes, and the ARMS primers are used for polymorphism detection of genes after being screened. The method is higher in detection sensitivity, stronger in specificity, more accurate and objective in result, simple in design and low in cost.

The present application discloses a method, kit and application for detecting gene polymorphism based on shared primer probes. In the method, the reporter group is labeled on the 5' ends of the wild-type ARMS primers and mutant ARMS primers designed in the same direction to prepare the probe-specific ARMS primers, which are used for gene polymorphism detection after screening, especially for Human CYP2C19 polymorphism detection. The method has simple design, low cost, higher detection sensitivity, stronger specificity, and more accurate and objective results.

The present application belongs to the field of detection of pathogenic microorganisms, and relates to PCR primers, detection methods, and applications of the detection methods for the detection of Staphylococcus korea. High detection primers, fluorescent quantitative PCR methods and detection kits. The primers include an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is the sequence shown in SEQ ID NO.1, the nucleotide sequence of the downstream primer is the sequence shown in SEQ ID NO.2, and the The PCR method includes at least obtaining the upstream primer and the downstream primer by Primer Premier 5 design according to the Staphylococcus korekii heat shock protein HSP60 gene, and the kit provides at least the upstream primer and the downstream primer, or using the PCR method.

The invention relates to a decidua and villus pairing cDNA chip. The cDNA chip is prepared through the following method that the decidua and villus tissue of a woman with early pregnancy abortion are taken, the RNA is extracted, cDNA is formed by reverse transcription and preset in a PCR reaction plate, and cDNA chip is made. A preparation method and application of cDNA chip are further provided. The method and application have the advantages that the decidua and villus tissue of every abortion woman are respectively taken, pairing is formed by cDNA, the cDNA pairs are concentrated on the same chip, and the function presentation of parent-fetal interface of the physiological structure is achieved on the chip outside the body. The decidua and villus pairing cDNA chip has the advantages of being high in throughput, strong in specificity, high in sensitivity, simple and fast in operation, high in quantitative accuracy, strong in result reliability, simple in result analysis, complete in clinical information for samples and the like, and the cDNA chip can be used for quantitative detection of the expression statuses of a target gene in different patients and for conducting in-depth correlation analyses of clinical information.

The present application belongs to the field of detection of pathogenic microorganisms, and relates to PCR primers, PCR methods and kits for detecting mouse adenovirus K87; specifically, the present application provides a pair of mouse adenovirus K87 strains with good specificity and high sensitivity detection primers, fluorescence quantitative PCR method and detection kit. The primers include an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is the sequence shown in SEQ ID NO.1, and the nucleotide sequence of the downstream primer is the sequence shown in SEQ ID NO.2; the The PCR method includes at least obtaining the upstream primer and the downstream primer through the design of Primer Premier 5 according to the hexon protein gene of the mouse adenovirus K87 strain; the kit provides at least the upstream primer and the downstream primer, or using the PCR method.