42results about How to "Analysis of results is simple" patented technology

The invention relates to a fluorescent polarization based homogeneous phase detection method of a single nucleotide polymorphism of a codon118 of an ERCC1 (excision repair cross-complementing 1) gene. The homogeneous phase detection method solves the problems of high cost, complicated operating steps, proneness to causing contamination and influence on detection result accuracy in the prior art, and is simple in steps, easy to operate, not prone to causing contamination as operation can be completed once in a closed tube, and low in cost as any special reagent and fluorescence quenching or micro-groove binding agent are not needed. Application of a specific probe with single end marked to detection of the single nucleotide polymorphism of the codon118 of the ERCC1 gene results in changes of fluorescent polarization values and enables detection results to be more objective and accurate. Result analysis is simple as only numeric comparison is needed in result judgment, and the homogeneous phase detection method can easily realize standardization and automation, is wide in application range and can be used for detecting clinical blood or tissue specimens.

The invention relates to a homogeneous phase detection method for a methylation state of an epidermal growth factor receptor (EGFR) gene promoter based on fluorescence polarization. The method solves the problems that the cost is high, the operation steps are fussy, pollution is easily produced and the accuracy of the detection result is affected in the prior art; the method is simple and easy to operate; and the method is finished by one step in a closed tube, and does not produce pollution. The method is low in cost, and does not require any special reagent and fluorescence quenching or micro groove bonding agent. Methylation of a target region is detected by using change of a fluorescence polarization value due to a single end-marked methylated specific probe, so that the detection result is objective and accurate. The method is simple in result analysis; and only digital comparison is required during result judgment, so the method easily realizes standardization and automation, has a wide application range and can be used for detecting clinical blood or tissue specimens.

The invention discloses a primer group, a method and a kit for detecting SLCO1B1 and APOE gene polymorphism based on a shared primer probe. According to the method, reporter groups are marked at 5'ends of wild ARMS primers and mutant ARMS primers which are designed in the same direction to prepare ARMS primers with probes, and the ARMS primers are used for polymorphism detection of genes after being screened. The method is higher in detection sensitivity, stronger in specificity, more accurate and objective in result, simple in design and low in cost.

The present application discloses a method, kit and application for detecting gene polymorphism based on shared primer probes. In the method, the reporter group is labeled on the 5' ends of the wild-type ARMS primers and mutant ARMS primers designed in the same direction to prepare the probe-specific ARMS primers, which are used for gene polymorphism detection after screening, especially for Human CYP2C19 polymorphism detection. The method has simple design, low cost, higher detection sensitivity, stronger specificity, and more accurate and objective results.

The present application belongs to the field of detection of pathogenic microorganisms, and relates to PCR primers, detection methods, and applications of the detection methods for the detection of Staphylococcus korea. High detection primers, fluorescent quantitative PCR methods and detection kits. The primers include an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is the sequence shown in SEQ ID NO.1, the nucleotide sequence of the downstream primer is the sequence shown in SEQ ID NO.2, and the The PCR method includes at least obtaining the upstream primer and the downstream primer by Primer Premier 5 design according to the Staphylococcus korekii heat shock protein HSP60 gene, and the kit provides at least the upstream primer and the downstream primer, or using the PCR method.