Primer sequence and test kit for gene detection of safe medication for children

Abstract

The invention discloses a primer sequence and a test kit for gene detection of safe medication for children, belonging to the technical field of molecular diagnosis. The test kit comprises 22 pairs ofamplification primers and can specifically amplify 22 common locus regions of the safe medication gene for children; the 22 single-base extension primers are used for detecting genotypes of 22 loci of the safe medication gene for children; in addition, the test kit comprises a special reagent for pretreatment and detection. The test kit provided by the invention can realize one-hole detection ofdifferent genotypes of 22 common loci related to clinical safe medication genes for the children, and has the advantages of high sensitivity, strong specificity, high accuracy, simple and convenient operation, low cost, high throughput, rapid detection, automatic result interpretation and easy clinical popularization and application. The test kit provided by the invention can be applied to gene detection of safe medication for the children, provides a reliable detection system and test kit for safe medication for the children, and has important clinical application value and good market application prospect.

Description

technical field [0001] The invention belongs to the technical field of molecular diagnosis, and in particular relates to a primer sequence and a kit for detecting children's safe medication genes based on a matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF) platform. Background technique [0002] Relevant data show that among the more than 3,500 kinds of pharmaceutical preparations in my country, the special dosage forms for children only account for 1.7%; relevant data from the State Food and Drug Administration show that in the drug clinical trial registration project, there are more than 160,000 domestic drug registration information, including children's drugs. There are only 2,698, accounting for only 1.63% of all drugs; children's drugs registered in clinical trials account for only 2.35%, while the number in developed countries in Europe and the United States exceeds 20%. At the 2018 China Children's Safe Medication Conference held recently, it w...

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Problems solved by technology

Fluorescence quantification combined with melting curve method has limited single-well detection throughput, and can only detect known mutations at a small number of sites. Specific primers need to be designed for each mutant genotype at each site; multi-hole and multiplex per well is required to cover common sites. Detection, multiple probe design requirements are high, the technology is difficult, the cost of detection probes is high, and the results are interpreted manually, which is prone to misjudgments; the reverse dot hybridization system needs to design probes for each locus and each genotype, and the cost High, the resolution depends on the effectiveness of the probe, it is prone to non-specificity, and the detection process is long, the operation is complicated, the system is open, and the samples are easily contaminated; the Sanger sequencing method is the gold standard for sequencing, but the process is long, the operation is complicated, and requires Manual interpretation of each site, covering common sites requires multi-hole detection, high cost, and low throughput; although high-throughput sequencing methods can cover multiple gene sites at one time, the cost of instruments and detection reagents is high, and the number of samples tested is high. When it is less, it will cause waste, resulting in higher costs, and the system requires a high nucleic acid sample volume (above 50ng), and the newborn heel blood sample volume is small and the concentration is low, often requiring screening and testing of multiple items, so it is very difficult It is difficult to obtain enough nucleic acid templates; the high-throughput sequencing detection process is as long as 3-5 days, the operation is complicated, and professional bioinformatics analysts are required to analyze the data, and the results are difficult to interpret, so it cannot be used as a screening item in local small and medium-sized Large-scale promotion and application in hospitals

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