Kit for detecting polymorphism of human ABCB1 gene and application thereof

Abstract

The invention relates to a kit for detecting polymorphism of a human ABCB1 gene and an application thereof. Substances in the kit comprise a specific primer and a specific fluorescent probe for detecting an rs1045642 SNP locus on the ABCB1 gene, Taq DNA polymerase, a dNTP mixed liquid, an MgCl2 solution, a fluorescence quantitative PCR reaction buffer solution and deionized water. The defects of various genetic mutation detection methods are overcome. The kit has the advantages of high detection accuracy, simple and convenient detection, short detection time, and simple result analysis, is suitable for use in clinical laboratories, can perform qualitative detection on a CYP2C19 gene polymorphism locus, performs PCR fluorescent amplification reaction according to the SNP locus, can judge a result just according to a condition whether two different fluorescence curves are started or no, cannot produce artificial errors, has low false positive and false negative rates, reduces detection costs, has no need for DNA extraction, can complete amplification only with cracking of complete blood cells, and improves the detection accuracy.

Description

technical field [0001] The invention relates to the gene detection technology in the clinical detection technology in the field of biomedicine, in particular to a kit for detecting human ABCB1 gene polymorphism and its application. Background technique [0002] Multi-drug resistance gene 1 (multi-drug resistance gene 1, MDR1 / ABCB1) is located on human chromosome 7 q21.1, its full-length cDNA is 4669bp, consists of 28 exons, and can be transcribed into a 4.5kb mRNA, encoding a 170kDa The transmembrane glycoprotein - P-gp. ABCB1 gene plays a leading role in the human multidrug resistance gene family, and its expression is affected by various factors, and it belongs to a regulated gene. The concept of individualized medicine has attracted more and more attention, and medical workers expect to optimize individualized treatment plans through gene prediction. In recent years, more than 50 SNPs of the ABCB1 gene have been discovered one after another, and the SNP site rs1045642 l...

AI Technical Summary

Problems solved by technology

Gene sequencing is currently the most widely used and more accurate method, but this method requires expensive equipment and professional personnel to operate, time-consuming and laborious, and it is difficult to promote in clinical practice

The gene chip method has the advantages of fast and high throughput, but the operation is complicated, there are many steps, the cost is high and it is not easy to popularize

The method of detecting gene mutation with restriction fragment length polymorphism (RFLP) has the advantage of low cost, but the process is cumbersome and takes a long time and is not suitable for popularization

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