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Paired cDNA arrays for decidua and villi

A technology of villi and decidua, which is applied in the field of paired cDNA chip of decidua and villi, which achieves the effects of high sensitivity, improved efficiency and strong reliability of results

Active Publication Date: 2020-09-01
夷希微医学科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no patents or literature reports on the technology and application of tissue cDNA chip preparation using decidua and villi tissue

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Preparation of cDNA chip (1)

[0026] 1. RNA extraction from human decidua tissue

[0027] Collect decidual and villi tissues during curettage, record the patient's age, BMI, number of miscarriages, treatment plan, endocrine, autoantibodies, blood coagulation, immunity, chromosome screening or hereditary thrombophilia screening data parameters, and establish clinical Information database. Using RNA extraction kit Oligotex TM RNA was extracted directly from the decidua and villi tissues of women who had miscarried in the first trimester. The decidual and villi tissues used were from Shanghai No. 1 Maternal and Infant Health Hospital (requirements: regular menstrual cycle of 28-32 days, definite last menstrual period, no history of abdominal pain and vaginal bleeding, no history of contraceptives and anti-early pregnancy drugs). The whole sampling process strictly complied with aseptic technique. The method and steps of the extracted RNA should strictly refe...

Embodiment 2

[0033] Example 2 Preparation of cDNA chip (2)

[0034] 1. RNA extraction from human decidua tissue

[0035]Collect decidual and villi tissues during curettage, record the patient's age, BMI, number of miscarriages, treatment plan, endocrine, autoantibodies, blood coagulation, immunity, chromosome screening or hereditary thrombophilia screening data parameters, and establish clinical Information database. Using RNA extraction kit Oligotex TM RNA was extracted directly from the decidua and villi tissues of women who had miscarried in the first trimester. The decidual and villi tissues used were from Shanghai No. 1 Maternal and Infant Health Hospital (requirements: regular menstrual cycle of 28-32 days, definite last menstrual period, no history of abdominal pain and vaginal bleeding, no history of contraceptives and anti-early pregnancy drugs). The whole sampling process strictly complied with aseptic technique. The method and steps of the extracted RNA should strictly refer...

Embodiment 3

[0041] Example 3 Preparation of cDNA chip (3)

[0042] 1. RNA extraction from human decidua tissue

[0043] Collect decidual and villi tissues during curettage, record the patient's age, BMI, number of miscarriages, treatment plan, endocrine, autoantibodies, blood coagulation, immunity, chromosome screening or hereditary thrombophilia screening data parameters, and establish clinical Information database. Using RNA extraction kit Oligotex TM RNA was extracted directly from the decidua and villi tissues of women who had miscarried in the first trimester. The decidual and villi tissues used were from Shanghai No. 1 Maternal and Infant Health Hospital (requirements: regular menstrual cycle of 28-32 days, definite last menstrual period, no history of abdominal pain and vaginal bleeding, no history of contraceptives and anti-early pregnancy drugs). The whole sampling process strictly complied with aseptic technique. The method and steps of the extracted RNA should strictly refe...

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Abstract

The invention relates to a decidua and villus pairing cDNA chip. The cDNA chip is prepared through the following method that the decidua and villus tissue of a woman with early pregnancy abortion are taken, the RNA is extracted, cDNA is formed by reverse transcription and preset in a PCR reaction plate, and cDNA chip is made. A preparation method and application of cDNA chip are further provided. The method and application have the advantages that the decidua and villus tissue of every abortion woman are respectively taken, pairing is formed by cDNA, the cDNA pairs are concentrated on the same chip, and the function presentation of parent-fetal interface of the physiological structure is achieved on the chip outside the body. The decidua and villus pairing cDNA chip has the advantages of being high in throughput, strong in specificity, high in sensitivity, simple and fast in operation, high in quantitative accuracy, strong in result reliability, simple in result analysis, complete in clinical information for samples and the like, and the cDNA chip can be used for quantitative detection of the expression statuses of a target gene in different patients and for conducting in-depth correlation analyses of clinical information.

Description

technical field [0001] The invention relates to the technical field of biochips, in particular to a paired cDNA chip of decidua and villi. Background technique [0002] Biochip refers to a high-throughput microarray of high-density tissues, cells, proteins, nucleic acids, sugars, and other biological components coated on solid supports such as silicon wafers and nylon membranes. Utilizing high-throughput biochips and specific molecular hybridization and staining techniques to detect the tested samples, a large amount of molecular detection information can be obtained accurately and quickly. At present, it has been widely used in the fields of life science research, molecular diagnosis of clinical medicine, and screening and verification of biological antibody-targeted drugs. [0003] Normal pregnancy is a complex physiological process. Although the fetus carries the HLA antigen inherited from the father, it does not trigger the mother's rejection of the special semi-allogen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C40B40/08C40B50/06C12Q1/6883
CPCC12Q1/6883C12Q2600/158C40B40/08C40B50/06
Inventor 鲍时华王柳柳朱丹
Owner 夷希微医学科技(上海)有限公司
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