Paired cDNA arrays for decidua and villi

Abstract

The invention relates to a decidua and villus pairing cDNA chip. The cDNA chip is prepared through the following method that the decidua and villus tissue of a woman with early pregnancy abortion are taken, the RNA is extracted, cDNA is formed by reverse transcription and preset in a PCR reaction plate, and cDNA chip is made. A preparation method and application of cDNA chip are further provided. The method and application have the advantages that the decidua and villus tissue of every abortion woman are respectively taken, pairing is formed by cDNA, the cDNA pairs are concentrated on the same chip, and the function presentation of parent-fetal interface of the physiological structure is achieved on the chip outside the body. The decidua and villus pairing cDNA chip has the advantages of being high in throughput, strong in specificity, high in sensitivity, simple and fast in operation, high in quantitative accuracy, strong in result reliability, simple in result analysis, complete in clinical information for samples and the like, and the cDNA chip can be used for quantitative detection of the expression statuses of a target gene in different patients and for conducting in-depth correlation analyses of clinical information.

Description

technical field

[0001] The invention relates to the technical field of biochips, in particular to a paired cDNA chip of decidua and villi. Background technique

[0002] Biochip refers to a high-throughput microarray of high-density tissues, cells, proteins, nucleic acids, sugars, and other biological components coated on solid supports such as silicon wafers and nylon membranes. Utilizing high-throughput biochips and specific molecular hybridization and staining techniques to detect the tested samples, a large amount of molecular detection information can be obtained accurately and quickly. At present, it has been widely used in the fields of life science research, molecular diagnosis of clinical medicine, and screening and verification of biological antibody-targeted drugs.

[0003] Normal pregnancy is a complex physiological process. Although the fetus carries the HLA antigen inherited from the father, it does not trigger the mother's rejection of the special semi-allogen...