Kit for detecting human COX-2 gene polymorphism and application thereof

Abstract

The invention relates to a kit for detecting human COX-2 gene polymorphism and an application thereof. The kit includes the following substances: a specific primer and a specific fluorescent probe for detecting an rs20417 SNP locus on COX-2 gene, a Taq DNA polymerase, a dNTP mixed liquid, a MgCl2 solution, a fluorescent quantitative PCR reaction buffer liquid and deionized water. The kit overcomes the defects in various gene mutation detection methods, is high in detection accuracy, is simple and convenient, is short in detection time and is simple in result analysis. The kit is suitable for clinical laboratories and can perform qualitative detection to COX-2 gene polymorphic sites, wherein a PCR fluorescent amplification reaction is carried out according to the SNP locus. A result can be determined just on the basis of whether two different fluorescent curves are positive or not, so that the kit is free of manual error, is low in false positive and false negative rate, and is improved in efficiency and reduced in detection cost.

Description

technical field [0001] The invention relates to the gene detection technology in the clinical detection technology in the field of biomedicine, in particular to a kit for detecting human COX-2 gene polymorphism and its application. Background technique [0002] Cyclo-oxygen-ase (COX) is an essential enzyme for the synthesis of prostaglandins (PGs), and is also a key rate-limiting enzyme in the initial step of PGs synthesis. COX has two isoenzymes COX-1 and COX-2. The human COX-2 gene is located in the 1q25.2-q25.3 region of the chromosome, with a length of about 8.3kb, including 10 exons and 9 introns, and encodes a total of 604 amino acids. There are some transcriptional regulatory sequences upstream of the 5′ terminal transcription initiation point, including 2 nuclear factor-κB (nuclearfactor-κB, NF-κB) sites and 2 activator protein-(2activatorportein-2, AP-2) sites , TATAbox sequence, cAMP response element (cAMPresponsiveelement, CRE), CCAAT enhancer binding protein si...

AI Technical Summary

Problems solved by technology

Gene sequencing is currently the most widely used and more accurate method, but this method requires expensive equipment and professional personnel to operate, time-consuming and laborious, and it is difficult to promote in clinical practice

The gene chip method has the advantages of fast and high throughput, but the operation is complicated, there are many steps, the cost is high and it is not easy to popularize

The method of detecting gene mutation with restriction fragment length polymorphism (RFLP) has the advantage of low cost, but the process is cumbersome and takes a long time and is not suitable for popularization

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