Kit for detecting human PEAR1 gene polymorphism and application thereof

Abstract

The invention relates to a kit for detecting human PEAR1 gene polymorphism and an application thereof. The kit includes the following substances: a specific primer and a specific fluorescent probe for detecting an rs2768759 locus on PEAR1 gene, a specific primer and a specific fluorescent probe for detecting an rs12041331 locus on the PEAR1 gene, a Taq DNA polymerase, dNTP mixed liquid, a MgCl2 solution, a fluorescent quantitative PCR reaction buffer liquid and deionized water. The kit overcomes the defects in various gene mutation detection methods, is high in detection accuracy, is simple and convenient, is short in detection time and is simple in result analysis. The kit is suitable for clinical laboratories and can perform qualitative detection to the two polymorphic sites, the rs2768759 and the rs12041331, on the PEAR1 gene, wherein a PCR fluorescent amplification reaction is carried out according to the SNP locus. A result can be determined just on the basis of whether two different fluorescent curves are positive or not without manual error, so that the kit is improved in efficiency and reduced in detection cost. The kit is free of DNA extraction, wherein a suspension liquid after cell pyrolysis is added to the reaction system to complete amplification.

Description

technical field [0001] The invention relates to the gene detection technology in the clinical detection technology in the field of biomedicine, in particular to a kit for detecting human PEAR1 gene polymorphism and its application. Background technique [0002] Platelet endothelial aggregation receptor 1 (PEAR1), a newly discovered transmembrane protein, is involved in the induction of platelet contact activation. After collagen acts on platelets, PEAR1 cytoplasmic tyrosine and serine residues are phosphorylated, so that platelets in contact with each other form a more stable thrombus. PEAR1 can regulate CRP-XL and adenosine diphosphate (ADP) formation pathway at the same time, the pathway is related to the two SNP sites rs2768759 and rs12041331 of the PEAR1 gene, and its gene polymorphisms may affect anti-platelet aggregation drugs (such as antiplatelet effect of aspirin, etc.). Through the detection of PEAR1 gene polymorphism, we can better understand the treatment effec...

AI Technical Summary

Problems solved by technology

Gene sequencing is currently the most widely used and more accurate method, but this method requires expensive equipment and professional personnel to operate, time-consuming and laborious, and it is difficult to promote in clinical practice

The gene chip method has the advantages of fast and high throughput, but the operation is complicated, there are many steps, the cost is high and it is not easy to popularize

The method of detecting gene mutation with restriction fragment length polymorphism (RFLP) has the advantage of low cost, but the process is cumbersome and takes a long time and is not suitable for popularization

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