Mixed bacteria producing biological surfactant, and screening method thereof

A technology of surfactant and screening method, which is applied in the field of microorganisms for oil production in oil fields, can solve the problems such as the lack of breakthrough progress, and achieve the effects of reducing screening costs, wide application and strong selectivity.

Inactive Publication Date: 2015-10-14
SUZHOU ZFA NEW ENERGY TECH CO LTD
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Problems solved by technology

However, how to screen mixed bacteria that produ

Method used

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  • Mixed bacteria producing biological surfactant, and screening method thereof
  • Mixed bacteria producing biological surfactant, and screening method thereof
  • Mixed bacteria producing biological surfactant, and screening method thereof

Examples

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Embodiment Construction

[0042] Below in conjunction with the examples, the specific implementation of the present invention will be further described in detail. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0043] One, the screening method of the mixed bacteria that produces biosurfactant of the present invention, comprises the following steps:

[0044](1) Activation of bacterial strains: Transfer the strains to be screened for oil production to the slant medium, and cultivate them at 37°C for 2 days, wherein the slant medium is: 3g of beef extract, 10g of peptone, 5g of sodium chloride, 20g of agar, 1000mL of distilled water, pH 7.0, steam sterilization at 121°C for 20 minutes, the oil production strains to be screened in this specific example include the preservation number CCTCC AB 205091 and the name Pseudomonas stutzeri (Pseudomonas stutzeri), the preservation number is CCTCC S2013441 and the name It is a...

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Abstract

The present invention relates to mixed bacteria producing a biological surfactant, and a screening method thereof, wherein the mixed bacteria is formed by mixing three strains, and the three strains respectively are Pseudomonas stutzeri having the preservation number of CCTCC AB 205091, Nocardioides ginsengagri having the preservation number of CCTCC S2013441, and Bacillus licheniformis having the preservation number of CCTCC AB 205141. According to the present invention, oil extraction strains to be screened are selected, the selected oil extraction strains to be screened are activated and cultured, the activated and cultured oil extraction strains to be screened are subjected to primary screening by using a blood flat plate method to obtain a primary screening fermentation broth, and the primary screening fermentation broth is re-screened by using an oil ring removing method so as to obtain the mixed bacteria; and the mixed bacteria integrates the advantages of the three stains and can produce the biological surfactant capable of being applied in the oilfield oil extraction, and the screening method has characteristics of wide application range, effective screening cost reducing, high accuracy, strong selectivity, simple process, easy operation, and short period compared with the prior art.

Description

technical field [0001] The invention relates to a microorganism used for oil recovery in an oil field, in particular to a mixed bacteria producing biosurfactant and a screening method thereof. Background technique [0002] Enhanced oil recovery is a kind of production technology that enhances oil recovery by improving the physical and chemical properties of oil reservoirs and oil reservoir fluids. Oil recovery technology can be roughly divided into primary oil recovery, secondary oil recovery and tertiary oil recovery. The primary oil recovery is carried out by using the natural energy in the oil layer; the secondary oil recovery is to use certain technology to restore the pressure of the oil layer to carry out oilfield development. The common ones are Oil recovery by water flooding; after that, the technology of enhanced oil recovery belongs to tertiary oil recovery. At present, except the Middle East, the main oil fields of the world's major oil-producing countries have e...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C12P19/44C09K8/584C12R1/38C12R1/10C12R1/01
CPCY02P20/59
Inventor 段浩
Owner SUZHOU ZFA NEW ENERGY TECH CO LTD
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