Alpha-amylase truncated body and application thereof

An amylase and variant technology, which is applied in the field of truncation of α-amylase, can solve problems such as increasing cost, and achieves a technology that is beneficial to industrial production, improves thermal stability and specific activity, large catalytic efficiency and thermal stability. Effect
CN102719418AInactive Publication Date: 2012-10-10GUANGXI ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGXI ACAD OF SCI
Publication Date
2012-10-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides an alpha-amylase truncated body and application thereof, and relates to meaningful alpha-amylase variant which simultaneously improves thermal endurance and catalytic activity, which is characterized in that 45-467 amino acid peptide fragment of bacillus subtilis Cn7alpha-amylase is cut off, and a fragment of artificially synthetic peptide is fused on C terminal. Obtained variant alpha-amylases respectively have temperature toleration increased by 2.7 DEG C, catalytic activity increased by 2.23 times compared with the parent alpha-amylase; and the variant alpha-amylases are more beneficial to industrialized production in the starch industry than the parent alpha-amylase.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a truncated body of α-amylase and its application. Background technique

[0002] Starch is a homopolymer composed of D-glucose monomers and is the major storage form of sugar in plants. As one of the most abundant and important biomass resources, starch is not only used for food, but also widely used in industries such as textiles, papermaking, and pharmaceuticals. It is especially used as a carbon source in the fermentation industry to produce fuel ethanol and amylase. Sugar, etc. The key to the development and utilization of starchy biomass resources is to degrade them into fermentable sugars such as glucose and maltose, which can be used for microbial fermentation to produce high value-added chemical products including organic alcohols and amino acids, including bioethanol, Biomass energy including bio-butanol, and bio-based materials including L-lactic acid, etc.

[0003] α-am...

Claims

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