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Method of reducing content of amylase in hybrid rice

A technology of amylose content and hybrid rice, applied in the field of rice genetics and breeding

Inactive Publication Date: 2015-03-11
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the difficulty in achieving this goal is to obtain the waxy mutation of Wx without changing other traits wx and improve wx dose

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Reducing the amylose content of the three-line high-yielding hybrid rice combination II You 3301

[0025] Method 1: Transforming the female parent II-32A into a waxy sterile line

[0026] The waxy rice variety Minyannuo, which is widely used in production, was selected and crossed with the maintainer line II-32B to obtain the hybrid F 1 seed; sow 1 F 1 Backcross with II-32B, get backcross generation B 1 f 1 seed; will B 1 f 1 For brown rice, select waxy brown rice grains for sowing, and sow II-32B at the same time, select the single plant with the most similar growth period, agronomic characteristics and maintainer line at the heading stage, and complete B 1 f 1 The second backcross with II-32B, get BC 1 f 1 seed; will BC 1 f 1 When the seeds are brown, select the glutinous brown rice grains for sowing, and sow II-32B at the same time. At the heading stage, select the single plant with the most similar growth period and agronomic characteristic...

Embodiment 2

[0029] Example 2: Reducing the amylose content of the three-line high-yielding hybrid rice combination Dyou 527

[0030] Method 1: Transforming the female parent D62A into a waxy sterile line

[0031] The waxy rice variety Minyannuo, which is widely used in production, was selected and crossed with the maintainer line D62B to obtain the hybrid F 1 seed; sow 1 F 1 Backcross with D62B, get backcross generation B 1 f 1 seed; will B 1 f 1Go out rough, select the glutinous quality brown rice grain among them to sow, sow D62B simultaneously, select the individual plant that growth period, agronomic character and D62B are most similar to D62B at the heading stage, complete B 1 f 1 The second backcross with D62B, get BC 1 f 1 seed; will BC 1 f 1 When the seeds are brown, select the waxy brown rice grains for sowing, and at the same time sow D62B. At the heading stage, select the single plant with the most similar growth period and agronomic characteristics to II-32B, an...

Embodiment 3

[0034] Example 3: Reducing the amylose content of the two-line high-yielding hybrid rice combination Liangyoupeijiu

[0035] Method 1: Transforming the female parent Pei'ai 64S into a waxy sterile line

[0036] The glutinous rice variety Ejingnuo 6, which is widely used in production, was selected to be crossed with Peiai 64S to obtain the hybrid F 1 seed; planting F 1 , Mixed harvesting of mature seeds F 2 ; sow F 2 , choose F 2 In the population, the most similar sterile plants in terms of growth period, plant and leaf morphology, agronomic traits, and sterility characteristics to Pei'ai 64S were cut off and regenerated, and single plants with the same fertility conversion characteristics as Pei'ai 64S were selected at the heading stage; In winter, Hainan is isolated and propagated, and a single plant is harvested for seedlings, and the waxy single plant is selected, which is the waxy photothermosensitive genic male sterile line (Peiai 64S wx S). Pei Ai 64S wx Com...

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Abstract

The invention discloses a method of reducing the content of amylase in hybrid rice and belongs to the technical field of rice genetic breeding. In the method, an original hybrid rice combination: one of a parent of a non-glutinous sterile line / a non-glutinous restorer is modified into a glutinous parent. The hybrid rice is prepared through a hybridization method of a glutinous sterile line / non-glutinous restorer or a non-glutinous sterile line / a glutinous restorer to obtain a hybrid rice variety in which the content of the amylase is lower than that of the original hybrid rice. In the invention, by means of a genetic effect and a genetic dosage effect of a glutinous gene wx, a near-isogenic line combination is reduced in the content of the amylase. In addition, a growth period, a plant and leaf form, panicle traits, floral organ traits, yield traits, disease resistant performance, adaptability and the like of the near-isogenic line combination are free of significant different when the near-isogenic line combination is compared with the original hybrid rice combination. Through quality analysis and taste evaluation, a new hybrid rice variety being appropriate in the content of the amylase and having the taste and the quality which are suitable for people in different region, thereby achieving an object of genetically improving a poor taste and a poor quality due to a high amylase content in the hybrid rice in the prior art.

Description

technical field [0001] The invention specifically relates to a method for reducing the amylose content of hybrid rice, and belongs to the technical field of rice genetics and breeding. Background technique [0002] In the past half a century, my country's rice breeding has experienced two technological innovations ahead of the world: one is the dwarf rice breeding that did not change the plant type as the main technical route in the 1950s, and the other is the early 1970s. Applied hybrid rice breeding with the utilization of heterosis as the main technical route. The two technological innovations have doubled my country's rice yield per unit area and total output, and have made significant contributions to meeting the growing population's demand for food, with remarkable results. In recent years, with the improvement of people's living standards and the adjustment of dietary structure, the consumption of rice has changed from "quantity" to "quality". Governments at all leve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 黄荣华范富英何水林章清杞官德义李美德杨帆
Owner FUJIAN AGRI & FORESTRY UNIV
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