Oxidation-resistant amylase mutant and preparation method and application thereof

A technology of oxidation resistance and amylase, applied in biochemical equipment and methods, botany equipment and methods, applications, etc., can solve the problems of difficult to obtain target strains, blindness, etc., and achieve shortened transformation time and efficient degradation , Strong oxidation resistance
CN102533697AActive Publication Date: 2012-07-04JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2012-07-04

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Abstract

The invention discloses an oxidation-resistant amylase mutant and a preparation method thereof, and belongs to the field of genetic engineering. The preparation method of the invention adopts Bacillus alcalophilus JN21 (CCTCC NO: M 2011231) amylase as a female parent, and performs site-directed mutagenesis of the Bacillus alcalophilus amylase sequence by molecular biology technology. Under the reconstruction conditions, the Bacillus alcalophilus amylase is treated at 40 DEG C in 500 mM H2O2 environment for 30 min, and the enzymatic activity residue is increased from 18% of a control (before mutation) case to 92%; with the strategy, the oxidation resistance of the amylase is greatly increased, which lays a foundation for the application of the amylase in industrial production fields of weaving desizing, washing agent additives, and the like. The strategy has important guidance meaning for the reconstruction of oxidation resistance of amylase and other enzymes.
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Description

Technical field

[0001] The invention relates to a mutant resistant to an oxidation resistant amylase and a preparation method thereof, in particular to an oxidation resistant amylase mutant and a preparation method thereof. Background technique

[0002] Alpha-amylase (EC 3.2.1.1) is an important industrial enzyme that degrades starch, mainly used in food, textile, medicine, detergent and other fields. Oxidation-resistant amylase can be used for textile desizing, detergent additives and viscosity modifiers using starch as a binder. Horikoshi first reported the alkaline amylase produced by the alkaliphilic bacteria A-40-2 in 1971. In 2007, Saxena et al. screened a strain that can produce alkaline amylase. 2010 Pancha et al. (Pancha I, Jain D, ShrivastavA, Mishra S, Shethia B, Mishra S, VP M, Jha BA thermoactive[alpha]-amylase from a Bacillus sp.isolated from CSMCRI saltfarm. International Journal of Biological Macromolecules, 2010 , 47(2): 288-291.) A strain producing temperatur...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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