Amylase mutant with improved heat stability and application thereof

An amylase and mutant technology, applied in the field of alkaline amylase mutants and their preparation, can solve problems such as limited application scope, and achieve the effects of broad application prospects, improved thermal stability, and efficient degradation
CN102994474AActive Publication Date: 2013-03-27JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2013-03-27

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Abstract

The invention discloses an amylase mutant with improved heat stability and application thereof and belongs to the field of genetic engineering. The amylase mutant uses Bacillus alcalophilus JN21 (CCTCC NO:M 2011231) amylase as a female parent, and a molecular biological technique is adopted to perform site-specific mutagenesis to a Bacillus alcalophilus amylase sequence. Under the transformation condition, the Bacillus alcalophilus amylase is improved from 15.3min (before mutation) to 43.8min at a 50-DEG C half-life period. By means of the application, the heat stability of the amylase can be improved remarkably, and a foundation is provided for industrial production of the amylase. The application has important guiding significance on modification of other enzymes.
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Description

technical field

[0001] The invention relates to an amylase mutant with improved thermostability and a method thereof, in particular to an alkaline amylase mutant with improved thermostability and a preparation method thereof. Background technique

[0002] Amylase is the earliest enzyme preparation used in industrial production, and it is one of the enzyme preparation products with the widest application and the largest output so far. Alkaline amylase has stability and activity in a high pH environment, so it has high application value in industrial production, and has been used in textiles, detergents, leather, paper, medicine, food and other industries. However, the unmodified alkaline amylase derived from wild strains has certain limitations in terms of thermal stability, which limits its application range. Therefore, based on the obtained expression platform of alkaline amylase in the large intestine, site-directed mutagenesis was used to carry out molecular modification...

Claims

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