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Kidney bean extract with high alpha-amylase inhibition activity as well as preparation method and application thereof

A technology of amylase inhibition and amylase inhibitor, which is applied in the field of preparation of plant active ingredients, can solve the problems of low economic value, time-consuming, complicated process, etc., and achieve high economic value, simple operation, and easy process production Effect

Pending Publication Date: 2021-04-06
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The pure kidney bean α-amylase inhibitor extraction method relies on chromatographic columns, the process is complicated, time-consuming, and the economic value is not high, which cannot meet the urgent needs of today's society. Relatively speaking, the preparation method of kidney bean crude extract is relatively simple and has Inhibiting the effect of α-amylase, but the extract is a mixture, which contains lectins that are harmful to the human body. Based on this, a suitable method is urgently needed to make the extract retain the inhibitory effect of α-amylase to the greatest extent. Decreased lectin activity

Method used

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  • Kidney bean extract with high alpha-amylase inhibition activity as well as preparation method and application thereof
  • Kidney bean extract with high alpha-amylase inhibition activity as well as preparation method and application thereof
  • Kidney bean extract with high alpha-amylase inhibition activity as well as preparation method and application thereof

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preparation example Construction

[0030] The invention provides a method for preparing a kidney bean extract with high α-amylase inhibitory activity without agglutination activity, comprising the following steps in sequence:

[0031] Under the conditions of pH 2-4 and temperature 30-70°C, use protease to treat the kidney bean aqueous solution for 0.5-2h, then adjust the pH and take the supernatant;

[0032] The supernatant was processed to obtain a powdered kidney bean alpha-amylase inhibitor extract.

[0033] In the above scheme, preferably, the amount of the protease is 1000-1500U / g sample.

[0034] In one embodiment of the present invention, preferably, the protease is acid protease.

[0035] In one of the embodiments of the present invention, as preferably, the preparation method for obtaining the kidney bean aqueous solution comprises the following steps:

[0036] Step 1, pretreatment of kidney beans: take kidney beans to make kidney bean powder of 40-80 mesh, use 400-1000mL of distilled water for every...

Embodiment 1

[0077] Five kinds of common kidney beans (black kidney beans, white kidney beans, milky kidney beans, red kidney beans and flower kidney beans) were crushed with a cyclone mill, passed through a 60-mesh sieve, and deionized water was used at a material-to-liquid ratio of 1:5 (w / v). Water extraction at room temperature for 2 hours, stirring at a speed of 200 rpm during the extraction process, after the extraction is completed, filter with 8 layers of gauze to remove the residue, and obtain the filtrate, which is left to stand at room temperature for 1 hour, after the starch precipitates, take the supernatant and centrifuge at 10,000 rpm for 30 minutes The supernatant was taken, frozen at -20°C, and freeze-dried.

[0078] Table 2 shows the results of the inhibition rate of α-amylase and the in vitro antioxidant capacity of 5 different kidney beans. The data showed that red kidney beans and flower kidney beans had the highest α-amylase inhibitor rate and the difference was not si...

Embodiment 2

[0082] Grind the flower kidney beans with a cyclone mill, pass through a 60-mesh sieve, and extract with deionized water at room temperature for 2 hours at a material-to-liquid ratio of 1:5 (w / v). During the extraction process, stir at a speed of 200 rpm. Remove the residue by filtering with a layer of gauze to obtain the filtrate. The filtrate was allowed to stand at room temperature for 1 hour. After the starch was precipitated, the supernatant was taken, centrifuged at 10,000 rpm for 30 minutes, and the supernatant was taken. After being frozen at -20°C, it was freeze-dried.

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Abstract

The invention discloses a preparation method of a kidney bean extract with no agglutination activity and high alpha-amylase inhibition activity. The method comprises the following steps: treating a kidney bean water solution for 0.5-2 hour by using protease under the conditions of pH2-4 and the temperature of 30-0 DEG C, regulating the pH value, and taking the supernatant; and treating the supernatant to obtain the powdery kidney bean alpha-amylase inhibitor extract. The invention also discloses a high alpha-amylase inhibition activity kidney bean extract without agglutination activity. The invention also discloses application of the acidic hydrolase in extraction of the high alpha-amylase inhibition activity kidney bean extract without agglutination activity. The method can remove agglutinin activity while keeping alpha amylase inhibition activity, has certain in-vitro antioxidant capacity, is simple to operate, high in economic value and easy for process production, and can meet the auxiliary treatment requirements of diabetic patients while meeting the requirement of common consumers for controlling weight.

Description

technical field [0001] The invention belongs to the technical field of preparation of plant active ingredients, and relates to a preparation method of kidney bean extract with high α-amylase inhibitory activity and no coagulation activity. Background technique [0002] Kidney bean, scientific name Phaseolus vulgaris Linn.sp, belongs to the leguminosae (Leguminosae) Phaseolus L., is widely planted and is one of the main beans consumed in the world. The perennial cultivation area of ​​kidney beans in my country is about 8 million mu, with a total output of about 800,000 to 900,000 tons, mainly distributed in Hei, Inner Mongolia, Hebei, Shanxi, Gansu, Xin, Sichuan, Yunnan, Guizhou and other places. [0003] Kidney beans contain a glycoprotein, α-amylase inhibitor, which is a pure natural active substance and belongs to a type of glycoside hydrolase inhibitors. It has been proven to effectively inhibit starch by forming enzyme-inhibitor complexes with enzymes Enzyme activity hi...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12P21/06
CPCC12P21/005C12P21/06
Inventor 刘丽娅佟立涛周素梅王丽丽汪云吉周闲容
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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