Milk allergen test plate and preparation method thereof
A technology of milk allergy and detection board, which is applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve problems such as inability to choose, achieve the effect of saving medical treatment costs, saving medical resources, and not easy to miss diagnosis
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[0031] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.
[0032] 1. Key raw materials
[0033] (1) Milk allergens: three pure allergens, casein, β-lactoglobulin, and α-lactalbumin, were all commercial reagents and were purchased from Sigma, USA.
[0034] (2) Nitrocellulose membrane (NC): commercial material, imported and repackaged, purchased from Beyotime Company;
[0035] (3) Anti-β-lactoglobulin antibody: purchased from Abcam Company.
[0036] ⑷ Colloidal gold particles: commercial reagent, purchased from Shanghai Haoran Biotechnology Co., Ltd., imported original, item number G1527
[0037] 2. Preparation of Milk Allergen
[0038] According to literature reports, milk mainly includes three allergen components of casein, β-lactoglobulin, and α-lactalbumin. Therefore, the present technology invention selects casein, β-lactoglobulin, and α-lactalbumin to be mixed in equal concentration ratios as the Know...
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