Milk allergen test plate and preparation method thereof

Abstract

The invention discloses a milk allergen test plate, which belongs to the gold immunochromatography detection. According to the milk allergen test plate disclosed by the invention, two ends of a PVC (polrvinyl chloride) base plate are respectively provided with a to-be-tested sample zone and an absorption zone. Colloidal gold mark antigens prepared by respectively marking colloidal gold into casein, beta lactoglobulin and alpha lactalbumin and mixing are orderly arranged between the sample zone to be tested and the absorption zone, and a nitrocellulose membrane is respectively provided with a detection zone coated with mixed milk allergen and a quality control zone coated with anti-beta lactoglobulin antibodies. In detection, color lines are formed in the detection zone and the quality contol zone when an immune complex is formed by specific milk antibodies contained in samples. If the samples do not contain specific milk antibodies, the detection zone does not display color, and only one color line is formed in the quality control zone. The milk allergen test plate disclosed by the invention with the design has the advantages of strong pertinence to the allergen detection, simplicity in operation, low cost and high sensitivity and the like, and can prevent the phenomenon of missed diagnosis in the single antibody detection. The milk allergen test plate is applied for rapidly screening patient allergic to milk, and is especially suitable for being used by primary medical treatment units.

Description

technical field [0001] The invention relates to a double-antigen sandwich gold immunochromatography detection technology, in particular to a milk allergen detection plate and a preparation method thereof. Background technique [0002] Allergic diseases refer to the body's wrong immune response to external stimuli (food, drugs, pollen, etc.), leading to various clinical symptoms. The immune response is a complex reaction process of the body after the antigen activates the immune cells; the antigen (protein) that causes the allergic reaction is called an allergen. Food allergic diseases are allergic gastroenteritis or skin symptoms caused by eating certain foods, sometimes leading to a severe systemic reaction - anaphylactic shock. Milk is an important source of nutrition for infants and even adults, and patients who are allergic to milk will have gastrointestinal symptoms and affect the healthy development of children. Milk allergy accounts for a considerable proportion of ...

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to solve the problem that in the screening process of ingestible allergens, the selection cannot be made according to the prompts of the clinician, and the detection can only be carried out according to the fixed combination method provided by the commercial kit, and IgG antibodies cannot be detected, which is easy to miss diagnosis. As well as enzyme immunoblotting, complex operation process and long reaction time, etc., a rapid milk allergen detection plate and its preparation method are provided

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