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SSR (Simple Sequence Repeat) molecular marker of new fruit mulberry variety 'Yueshen 201', core primer group of SSR molecular marker, kit and application of SSR molecular marker, or core primer group or kit

A technology of molecular markers and core primers, which is applied in the identification of variety resources and germplasm innovation, can solve problems affecting the effectiveness and authority of new variety identification, large environmental impact, and long test cycle, and achieve reliable and intuitive results. The effect of high efficiency and overcoming uncertainty

Active Publication Date: 2021-11-12
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0005] The traditional identification of new mulberry varieties is mainly based on phenotypic traits, which is greatly affected by the environment, poor stability, and long testing cycle, which seriously affects the effectiveness and authority of new variety identification

Method used

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  • SSR (Simple Sequence Repeat) molecular marker of new fruit mulberry variety 'Yueshen 201', core primer group of SSR molecular marker, kit and application of SSR molecular marker, or core primer group or kit
  • SSR (Simple Sequence Repeat) molecular marker of new fruit mulberry variety 'Yueshen 201', core primer group of SSR molecular marker, kit and application of SSR molecular marker, or core primer group or kit
  • SSR (Simple Sequence Repeat) molecular marker of new fruit mulberry variety 'Yueshen 201', core primer group of SSR molecular marker, kit and application of SSR molecular marker, or core primer group or kit

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Experimental program
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Embodiment 1

[0033] Example 1 Obtained by Screening of Specific Primer Sequences of New Fruit Mulberry Variety 'Yuechen 201'

[0034] 1. Selection of SSR primers

[0035] Use MISA software to search the genome sequence in the mulberry genome database, and retrieve the SSR sites in the genome sequence. The length of the retrieved SSR motif repeating unit is 2 to 6 nucleotides, and set 2, 3, and 4 respectively , 5, 6 nucleotides, the minimum number of search repetitions is 6, 5, 5, 4, 4 times, and the length of the flanking sequence of the SSR site is ≥ 150 bp, according to the searched SSR site, use Primer3v2. 3. 4 (http: / / primer3.sourcego-rge.net) software to design SSR primers, the design criteria are: the length of the primer is 18-25bp, the annealing temperature is 57-63°C, the GC content is 40-70%, the length of the PCR product 100-300bp, randomly select SSR sites that can be compared with the NCBI non-redundant protein database to synthesize 300 pairs of primers, each pair of primers...

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Abstract

The invention discloses an SSR (Simple Sequence Repeat) molecular marker of a new fruit mulberry variety 'Yueshen 201', a core primer group of the SSR molecular marker, a kit and an application of the SSR molecular marker, or the core primer group or the kit. A fluorescent SSR technology is utilized, 300 pairs of SSR primers developed through mulberry genome sequence design are screened, it is confirmed that core primers marked with M20310 and M252 can amplify two specific bands for the new fruit mulberry variety 'Yueshen 201' respectively, the new fruit mulberry variety 'Yueshen 201' can be identified from fruit mulberry strains respectively, and the new fruit mulberry variety 'Yueshen 201'can be identified from the fruit mulberry strains respectively. The molecular marker can be used for rapid identification and detection of the new fruit mulberry variety 'Yueshen 201'. According to the invention, the M20310 marker and / or the M252 marker of the 'Yueshen 201' are / is directly detected to identify the 'Yueshen 201', the uncertainty of external morphological characteristics on identification of the 'Yueshen 201' is overcome, the operation is simple, the detection efficiency is high, the result is reliable and visual, a scientific technical guarantee is provided for protection of the new fruit mulberry variety 'Yueshen 201', and the molecular marker, the core primer group and the kit are beneficial to popularization, utilization and protection of the varieties.

Description

technical field [0001] The invention relates to the technical field of identification of variety resources and germplasm innovation, in particular to the SSR molecular marker of a new fruit mulberry variety 'Yuechen 201' and its core primer set, kit and application. Background technique [0002] Fruit mulberry is the purpose of mulberry fruit production, and it is collectively referred to as mulberry trees for fruit or leaf use. It is one of the important types of rich mulberry germplasm resources in my country. As a new type of fruit tree, fruit mulberry is loved by people because of its unique taste, rich nutrition and high active substances. The planting area and area are constantly expanding, and it has shown a good development prospect. In addition to fresh food, mulberries are processed and developed into mulberry juice, mulberry wine, mulberry jam, mulberry red pigment, mulberry vinegar and other products. The mulberry industry has begun to take shape and has shown a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125C12Q2563/107
Inventor 王振江罗国庆唐翠明钟建武林森陈莲
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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