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The invention belongs to the technical field of animal husbandry medicines, and particularly relates to a wormwood extract, a preparation method and application of the wormwood extract in preparation of a medicine for preventing foot-and-mouth disease of beef cattle. The method specifically comprises the following steps: adding artemisia desertorum into an acetic acid aqueous solution, boiling at 60-100 DEG C for specified time, filtering and cooling; and adding the cooled artemisia desertorum into an ethanol solution, soaking for a second time at room temperature, and carrying out material-liquid separation to obtain a leaching solution, namely the artemisia desertorum extract. The wormwood extract can prevent cattle from being infected with the foot-and-mouth disease, in the high-incidence period of foot-and-mouth disease infection, the morbidity of cattle taking the wormwood extract is only 10%, the morbidity is reduced by 10% compared with that of cattle taking common diet (the morbidity is 20%), the foot-and-mouth disease of cattle is effectively prevented, and no adverse effect is generated on growth and development of the cattle. In addition, the wormwood is wide in source and low in cost, and the preparation method is simple, convenient and suitable for large-scale breeding and popularization.