56results about How to "Growth and development effects" patented technology

The invention relates to a method and a device for extracting heavy metal ions from soil, which are used for extracting heavy metal ions from the heavy metal polluted oil. The method comprises the following steps of: forming a heavy metal ion adsorbent by adopting a compound containing carbon nano fibers, active carbon, a cationic surface active agent, acrylamide, potassium acrylate, sludge particles and potassium sulfide; putting the heavy metal ion adsorbent in a porous hard shell to form a porous hard plastic bag; and embedding the porous hard plastic bag into soil for extracting the heavy metal ions. The method and the device for extracting the heavy metal ions from soil aim at the problem of severe pollution of Cd, Cr, Cu, Hg, Pb and Zn of the soil and can effectively absorb, passivate, solidify and extract active ions such as Cd2+, Cr3+, Cu 2+, Hg2+, Pb2+, Zn2+, and the like in plow layers of the soil, maintain microzone water of the soil, release a certain K+ ions and realize the update, the purification and the circular treatment of the absorbent in the device for extracting the heavy metal ions.

The invention provides a method for finishing down producing goat VEGF gene fixed-point knock-in mediated by a CRISPR/Cas9 system. The method comprises the following steps: establishing a gRNA expression vector and a VEGF homologous recombination vector on the basis of the CRISPR/Cas9 system according to a CCR5 gene sequence of down producing goats; then jointly transferring three optimized vectors into fibroblasts of down producing goat fetuses to obtain VEGF gene fixed-point knocked-in positive cells; and preparing VEGF gene fixed-point integrated down producing goats by using a somatic nuclear transfer technology. The established targeting vector based on the CRISPR/Cas9 system provides a simple, rapid and safe path for fixed-point knock-in of goat VEGF genes. The method does not involve any screening marker genes in a cell line screening process, therefore, the safety of transgenic animals is greatly improved, and the method has high value on research of genetic breeding and gene functions of down producing goats.

The economic and practical artificial feed is suitable for scale feeding of beet noctuids to produce beet noctuids virus pesticide. It is produced by mixing the following materials: soybean powder 7-10 wt%, yeast powder 3-5 wt%, wheat bran 2-5 wt%, agar 1.5-3 wt%, proper amount of supplementary material and water. Feeding beet noctuids with the artificial feed can result in average chrysalis weight of 117.8 mg, eclosion rate of 99 %, high larva livability, normal growth of chrysalis and oviposition amount and egg incubation rate reaching those of natural feed. Being low in cost, practical andsimple in production, the feed of the present invention can meet the requirement of feeding beet noctuids in industrial scale.

The invention relates to an externally applied traditional Chinese medicine composition for treating dysmenorrhea, as well as a preparation method and an application thereof. The externally applied traditional Chinese medicine composition comprises the following active pharmaceutical ingredients in parts by weight: 5-10 parts of nutgrass galingale rhizome, 5-10 parts of argy wormwood leaf, 3-5 parts of Chinese angelica, 3-5 parts of dahurian angelica root, 1-4 parts of officinal magnolia bark, 3-5 parts of motherwort herb, 0.5-2 parts of Chinese eaglewood and 3-6 parts of turmeric. The optimized scheme is as follows: one or more of 1-4 parts of white paeony root, 3-5 parts of Szechuan lovage rhizome, 2-5 parts of rhizoma corydalis, 1-4 parts of tree peony bark, 0.1-0.6 part of manchurian wildginger, 0.05-0.2 part of saffron and 0.2-0.8 part of flos caryophyllata are added. The externally applied traditional Chinese medicine composition disclosed by the invention can improve the comprehensive effects for treating the dysmenorrhea and simultaneously play the roles in inhibiting the contraction of uterine smooth muscle, strongly reliving the spasm of the uterine smooth muscle, alleviating pain, inhibiting swelling, promoting blood circulation, removing blood stasis, dilating blood capillaries and the like. Simultaneously, the externally applied traditional Chinese medicine composition disclosed by the invention has smaller toxicity and side effects and can not cause allergic reactions. The invention further provides the preparation method of the medicament and the application thereof in preparation of externally applied medicaments for treating the dysmenorrhea.

The invention relates to a bacillus amyloliquefaciens for preventing and curing strawberry wilt and application thereof. The bacillus amyloliquefaciens JM-3 is deposited at the general microorganism center of China Committee for Culture Collection of Microorganisms on March 12, 2018, the deposition address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, yard 1, beichen west road,Chaoyang District of Beijing, and the deposition number is CGMCC No.15444. Microbial preparation prepared by fermentation broth of the bacillus amyloliquefaciens JM-3 has a lower moisture content, bacterial cells are in a semi-dormant state, spores have high stress resistance, and the storage period of the preparation can be up to 2 years; and the microbial preparation of the bacillus amyloliquefaciens JM-3 can effectively reduce the occurrence of strawberry wilt and reduce the amount of chemicals used.

The invention relates to the field of agricultural planting and soil conditioners, in particular to a special saline alkali soil conditioner for facility agriculture. Agricultural wastes such as cotton straws are used as main components and carriers of the saline alkali soil conditioner, various natural phytoncides and insecticides are screened, and the compositions of the various natural phytoncides and insecticides are optimally matched, and finally the saline alkali soil conditioner consisting of cotton straws, garlic extract, Marigold extract, Sichuan Pepper extract, Cacumen biotae powder,kieselguhr and humic acid. The soil conditioner is low in cost, convenient to operate, safe to use, stable and long-acting, the structure of facility saline alkali soil can be improved obviously, thefertilizer is saved by 30% to 50%, and the cost is reduced by more than 80%; and moreover, a new path is provided for recycling the cotton straws, the natural resources are saved, the energy crisis is relieved, and the protection for ecological environment is also facilitated.

The invention provides a method for preparing cordyceps militaris, comprising the following steps of preparing strains, preparing a cultivation substrate, cultivating a strain-receiving substrate, and harvesting and drying after the strains are cultivated maturely, wherein the step of preparing the strains is performed by means of cultivating cordyceps militaris strains in the substrate, cultivating by utilization of a rotary swing bed, and cultivating at a constant temperature for a certain time till mycelium pellets are formed and uniformly float; in the step of preparing the cultivation substrate, the cultivation substrate is composed of a substrate body and a culture solution; the ingredients of the culture solution comprise beans, coconut powder, pollen, glucose, magnesium sulfate, vitamin B1, vitamin B6 and the rest of alkalescent water; the material-to-liquid ratio of the substrate body to the culture solution is 1:1.7-1.9; the moisture content of a dried product is not greater than 8%. The cordyceps militaris prepared according to the method for preparing the cordyceps militaris has the advantages that the color is golden, conidial powder is contained, the contents of cordycepin, cordyceps polysaccharide and superoxide dismutase (SOD) are high, the formula and method provided by the invention are simple and are easily obtained, the ingredients which are used for preparing the culture solution are simple and are easily obtained, and the cost is low.

The invention discloses cloning and application of an R gene TN2 for regulating and controlling powdery mildew resistance of plants. The invention provides a method for regulating and controlling the resistance of plants on powdery mildew. An amino acid sequence of TN2 protein is a sequence 2 in a sequence table; premature translation termination is caused by single amino acid change of seven sites and mutation of two sites; and the resistance of an exo70B1 mutant on the powdery mildew and powdery mildew-induced cell death can be restrained, but no obvious effect is generated on growth and development of plants, thus, the TN2 can be applied to the technical field of genetic engineering, so as to regulate and control resistance reaction of the plants on the powdery mildew.

The invention relates to the fields of agricultural planting and soil improvement agents and in particular relates to a continuous cropping resistant composite soil improvement agent special for facility agriculture. Agricultural wastes, namely wheat straws, are taken as a principle component and a carrier of the continuous cropping resistant composite soil improvement agent, multiple natural phytoncides and insecticides are screened, the formula composition is subjected to proportional optimization, and finally the continuous cropping resistant composite soil improvement agent composed of thewheat straws, garlic extract, radix stemonae extract, heartleaf houttuynia herb extract, nano chitosan and composite trace elements is obtained. The soil improvement agent disclosed by the inventionis low in cost, convenient to operate, safe to use, stable and long-acting, provides a novel pathway for recycling of the wheat straws and contributes to saving natural resources, easing the energy crisis and protecting the ecological environment.

The invention relates to the field of agriculture planting and soil conditioners and especially relates to a clayey soil conditioner specially used for facility agriculture. In the invention, an agricultural waste, soya bean straw, is used as the main component and carrier of the clayey soil conditioner; by screening various natural phytoncides and insecticides and optimizing the formula of the clayey soil conditioner, the clayey soil conditioner, which is composed of soya bean straw, garlic extract, ageratum conyzoides extract, stellera chamaejasme extract, cacumen biotae leaf powder and diatomite, is produced; the clayey soil conditioner is low in cost, is convenient and safe to use, is stable and long-acting, can significantly improve facility clayey soil structure, and can save fertilizer by 30-50% and reduce the cost by more than 80%; meanwhile, the invention provides a new approach for cyclic utilization of the soya bean straw, so that natural resources are saved, energy crisis is alleviated and ecologic environment is protected.

The invention belongs to the field of agricultural preparations and in particular relates to herbicidal quizalofop-p-ethyl missible oil containing synergistic vegetable oil and a preparation method ofthe herbicidal quizalofop-p-ethyl missible oil. The herbicidal quizalofop-p-ethyl missible oil containing synergistic vegetable oil comprises the following raw materials in percentage by weight: 1-20% of quizalofop-p-ethyl, 5-50% of synergistic vegetable oil, 1-3% of synergistic aids, 7-35% of an emulsifier and 20-50% of a cosolvent. The vegetable oil serves as a pesticide solvent and is safe toproduce and store. The herbicidal quizalofop-p-ethyl missible oil is small in irritation to producers and users and low in decomposition rate and does not have any adverse influence on growth and development of soybean and rape seedlings, the liquor drying time is obviously prolonged, and the deposit quantity of the agent is improved. The content of aromatic organic solvents in the conventional missible oil is 65-80%, while the content of the solvents in the missible oil disclosed by the invention is decreased to 20-50%; and therefore, the pollution of the organic solvents to the environment is reduced. Moreover, the herbicidal quizalofop-p-ethyl missible oil containing the synergistic vegetable oil disclosed by the invention is simple in preparation method, mild in conditions, green and environment-friendly.

The invention relates to the field of agricultural planting and vegetable cultivating, in particular to a peanut straw seedling growing medium special for vegetables and a preparing method of the peanut straw seedling growing medium. Agricultural waste (peanut straw) is utilized as a main raw material of a vegetable seedling growing medium, and by studying the influence of peanut straw biologicalcomposting, an optimized medium proportion, physical and chemical parameter regulation and all medium compositions on vegetable seedling growth and development in combination with the preparing methodand screening of conditions, the ideal peanut straw seedling growing medium is researched. The peanut straw seedling growing medium is simple in composition, safe to use, low in production cost and simple in process technology, is beneficial to being applied and popularized in the field of vegetable planting and provides a new path for comprehensive utilization of peanut straw resources.

The invention provides a greenhouse fill-light seedling raising shelf. A supporting frame is provided with a plurality of layers of fixed plates and containing tanks, seedling raising trays are arranged in the containing tanks, and the outer side of each seedling raising tray is provided with a driven gear; the fixed plates penetrates through a rotating shaft, and the rotating shaft is connected with a drive motor; the rotating shaft is provided with gears which mesh with the driven gears; each seedling raising tray is provided with a top plate, a bottom plate and a water supply cavity arranged between the top plate and the bottom plate, and the water supply cavity is connected with a water supply inlet pipe; the top plate is provided with a plurality of planting holes, and the inside of each planting hole is provided with a planting slot of which the lower end is positioned in the water supply cavity; the bottom of the planting slot is provided with a filtration hood, a siphoning diversion column is arranged in the filtration hood, and the lower end of the siphoning diversion column extends to the inside of the water supply cavity; the inner wall of the planting slot is provided with a clamping part, and a filtration plate is clamped on the clamping part and divides the planting slot into a seedling raising region and a conduction region; the conduction region is filled with a conduction substrate; a seedling raising ring is arranged in the seedling raising region, and filled with a seedling raising substrate; the side wall of the seedling raising region is provided with a plurality of gas ports which communicate between the seedling raising region and the water supply cavity; the lower end of the water supply cavity is connected with a drain pipe; and a cross rod is arranged above the seedling raising tray, and provided with fill-light lamps. The greenhouse fill-light seedling raising shelf can ensure the moisture content during seedling raising, is convenient for transplantation, and has the advantages of uniform lighting and longer lighting time.

The invention relates to the technical field of biological information, and discloses a method for noninvasive identification of pseudo-female Chinese softshell turtle through fluorescent quantitativePCR. The method comprises the following specific steps of setting a pair of primer groups for sexual identification of the pseudo-female Chinese softshell turtle, wherein the primer groups comprise primer pairs designed by DNA sequences of target genes 18S rRNA and Gapdh; performing fluorescent quantitative PCR data processing by adopting a 2-[delta][delta]Ct method, using the Gapdh as a reference gene, and calculating the relative copy number of the 18S rRNA in a genome; and by utilizing the fact that the DNA sequence copy number of the 18S rRNA on the W chromosome of the Chinese softshell turtle is higher than that on the Z chromosome, screening out the pseudo-female Chinese softshell turtle with the ovarian gonad and the ZZ genotype. According to the identification method, the fluorescent quantitative PCR technology is used for sexual identification of the Chinese softshell turtle, detection can be achieved only through a trace amount of blood, non-invasive collection of tail bloodof the Chinese softshell turtle has no influence on health of the Chinese softshell turtle, the method has the characteristics of being efficient, high in sensitivity, good in stability and high in specificity, the blank of pseudo-female Chinese softshell turtle identification is filled, breeding of all male softshell turtle fries is facilitated, and the breeding benefit is improved.

The invention was involved in the specific promoter of phloem. This promoter was separated from DNAbeta accompanied by China tomato yellow leaf curl virus (TYLCV-CHI). The separated promoter was the sequence of whole promoter of beta ClORF and part of deletion promoter from DNAbeta. The experiment shown that the promoter of beta ClORF from TYLCV-CHI DNAbeta was the specific promoter of the phloem. The invention introduced whole promoter of beta ClORF from TYLCV-CHI DNAbeta, deletion promoter sequence and expression box containing whole promoter of beta ClORF and deletion promoter sequence.

The present invention provides a high quality provenance feed for predatory mites. The feed comprises the following components in weight percentages: 65-75% of bran, 7-13% of pollen, 5-15% of milk powder and 5-15% of an antibacterial agent. After a consumption of the feed, the predatory mites are rapid in development, easy to breed, good in predation ability and strong in adaptability.

The invention provides a greenhouse seedling raising rack. A supporting frame is provided with plurality of layers of fixing plates and accommodating grooves in an erected mode and is provided with built-in seedling raising trays. Driven teeth are arranged on the outer side of the seedling raising trays, through holes are formed in one side of each fixing plate, and rotary shafts penetrate into the through holes. The rotary shafts are connected with a driving motor. Gears meshed with the driven teeth are arranged on the rotary shafts. The seedling raising trays are provided with top plates, base plates and water supply cavities between the top plates and the base plates, and the water supply cavities are connected with feed water inlet pipes. A plurality of planting holes are formed in the top plates, planting grooves are formed in the top plates, and the lower ends of the planting grooves are located in the water supply cavities. Clamp-connecting parts are arranged on the inner walls of the planting grooves, filter plate bodies are clamp-connected to the clamp-connecting parts, and the planting grooves are divided into seedling raising zones and conduction zones. A conduction medium is contained in the conduction zones. Seedling raising loop sleeves are arranged in the seedling raising zones, and a seedling raising medium is contained in the seedling raising loop sleeves. A plurality of air guiding holes are formed in the side walls of the seedling raising zones and are communicated with the seedling raising zones and the water supply cavities, and the lower ends of the water supply cavities are connected with drainage pipes. The greenhouse seedling raising rack can ensure moisture content during seedling raising and transplantation and makes lighting uniform.

The invention discloses a tomato plant hooking device for tomato cultivation, and the device comprises a main hanger, auxiliary hangers, a cross liquid supply pipe, a liquid guide pipe, and a hangingdisc. One end of the device is provided with two auxiliary hangers. One side of the main hanger is provided with one auxiliary hanger in a parallel manner, and the main hanger and the three auxiliaryhangers are each of a circular structure. A hollow cylindrical cross liquid supply pipe is disposed above a part between two auxiliary hangers at one end of the device. The beneficial effects of the invention lie in that the tomatoes on the tomato plants are respectively put in fruit hoppers of a plurality of fruit loading discs on a side wall of the main hanger, thereby guaranteeing that the hollow cylindrical fruit hoppers provide supporting forces for tomatoes, guaranteeing that the tomatoes cannot cause the bending of the branches of the tomato plants, guaranteeing that the growth of the trunks of the tomato plants cannot be affected, enabling the growth of the branches to be more natural and straight, and bringing convenience to a worker for the management of the fruits of the tomatoes; the device is convenient to operate, is firm and reliable, is good in social benefits and economic benefits, and is suitable for the popularization and application.