48 results about "Avian orthoreovirus" patented technology

The present invention relates to novel strains of avian reovirus that were isolated from severe cases of Runting Stunting Syndrome in young broiler chickens in southeast United States. The invention is directed to avian reoviruses that impair digestion in poultry, diagnostic assays using nucleotide- or amino acid-specific components of such viruses, and to vaccines that protect chickens from disease caused by such viruses. Nucleotide sequences for the SI gene, encoding the sigma C minor outer capsid protein, were amplified, and the nucleotide and predicted amino acid sequences were compared with sequences from other recently isolated reovirus field isolates and vaccine strains. Antigenic and molecular characterization of the newly isolated reoviruses revealed a lack of homogeneity with current U.S. isolates, with less than 60% percent amino acid similarity across the sigma C protein. Sequence comparisons with previously reported malabsorption isolates from Europe and Asia revealed a higher amino acid similarity, approaching 80%.

The invention provides a GeXP quick detection kit and detection method for simultaneously identifying 8 chicken infectious immunosuppression disease pathogens. The kit is used on the basis of a GeXP system, and comprises 8 pairs of PCR (polymerase chain reaction) primers. The detection result indicates that the kit has the advantages of high specificity and high sensitivity and can be used for simultaneously identifying and detecting chicken Marek's disease virus, A, B and J subgroup avian leucovirus, avian reticuloendothelium hyperplasia virus, avian reovirus, chicken infectious anemia virus and infectious bursal disease virus.

The invention discloses a bi-fluorescence quantitative RT-PCR detection kit for mycoplasma synoviae and avian reovirus. The kit comprises a primer group and a probe group, wherein the primer group comprises a primer 1, a primer 2, a primer 3 and a primer 4, which respectively have base sequences of sequence tables SEQ.ID.No.1 and 2, and SEQ.ID.No.4 and 5; and the probe group comprises a probe A and a probe B which respectively have the base sequences of sequence tables SEQ.ID.No.3 and 6. Experiments prove that the kit disclosed by the invention has the advantages of short detection time, high detection sensibility and strong specificity, two pathogens of the mycoplasma synoviae and the avian reovirus can be detected and discriminated at the same time, and the corresponding pathogeny content can be quantified, so that the kit can be used for assessing the curative effects of vaccines and medicines on the mycoplasma synoviae and the avian reovirus and researching pathogenic mechanisms and other respects; therefore, the kit disclosed by the invention has an important significance on prevention and control of the mycoplasma synoviae and the avian reovirus.

The invention discloses a multiple fluoroimmunoassay primer, kit and method for rapidly distinguishing 5 avian immunosuppression pathogens. Operation is simple, a target amplified fragment is acquired through a PCR, an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized, the MFI value is read through a detector, and viruses of different types are distinguished. The method can simultaneously detect the chicken infectious anemia virus, the chicken Marek's disease virus, the avian reticuloendotheliosis virus, the avian reovirus and the chicken infectious bursa disease virus accurately, specificity is high, sensitivity is high, and repeatability is good. Compared with a traditional detection method, the method achieves simultaneous detection of multiple different target molecules in the same sample, the use number of samples is small, operation is simple and rapid, and the detection cost can be greatly reduced.

The present invention relates to novel strains of avian reovirus that were isolated from clinical cases of viral arthritis/tenosynovitis in chickens in the southeast United States. The invention is directed to these novel group 1 and group 2 avian reoviruses, diagnostic assays using antibodies and/or nucleotide- or amino acid-specific components of such viruses, such as the S1 gene encoding the sigma C protein, and to vaccines that protect chickens from disease caused by such viruses.

Methods for treating proliferative disorders, by administering reovirus to a ral-mediated proliferative disorder, are disclosed. The reovirus is administered so that it ultimately directly contacts target cancer cells. Proliferative disorders include but are not limited to neoplasms. Human reovirus, non-human mammalian reovirus, and/or avian reovirus can be used. If the reovirus is human reovirus, serotype 1 (e.g., strain Lang), serotype 2 (e.g., strain Jones), serotype 3 (e.g., strain Dearing or strain Abney), other serotypes or strains of reovirus, and recombinant reovirus can be used. Combinations of more than one type and/or strain of reovirus can be used, as can reovirus from different species of animal. Either solid neoplasms or hematopoietic neoplasms can be treated.

The invention provides a preparation method for avian reovirus virus water-in-oil-in-water type inactivated vaccines. The preparation method comprises the following steps: preparation of chicken embryo fibroblast, cultivation of viruses, inactivation of the viruses, emulsification of the vaccines, and the like. Compared with the prior art, locally separated avian reoviruses are adopted, so that the immunogenicity is good, and the relatively high protection rate is adopted; the chicken embryo fibroblast is adopted to replace the utilization of a chicken embryo chorioallantoic membrane in a inoculation method for breeding the avian reoviruses, so that the vaccine preparation cost is saved, the mass discrepancy between each batch is lowered, the yield is high, and the viral titer and the vaccine quality are improved; the vaccines are subjected to emulsification by using a homogenizer, the prepared vaccines are water-in-oil-in-water type; compared with traditional water-in-oil type vaccines, the vaccines provided by the invention have low viscosity, small granularity, and easy absorption; surface active agent compositions are not required to be added in the preparation method by selecting and using an adjuvant, so that the prepared vaccines have good safety, and avoid side effects caused by difficult absorption of a traditional oil adjuvant.

The invention provides a preparation method of an avian reovirus multi-subunit vaccine. According to the technical scheme, prokaryotic expression is conducted aiming at group-specific neutralizing antibody antigens and type-specific neutralization reaction surface antigens mainly generated in a standard virus strain S1133 of an avian reovirus, all the antigens are matched according to a certain ratio, an immunopotentiator is added, an immune response of an organism can be fully stimulated, different antigen proteins cause the organism to generate different specific antibodies, the antibody cross protection range is expanded, and a good protection effect is achieved on clinical breeding of large poultry. The antigens in the vaccine prepared through the method are pure, inactivator is not used, and therefore immune side effects are reduced; meanwhile, the antibody level is high, clinical protection is good, the vaccine has the advantages of being low in cost and easy to produce on a large scale, and accordingly the vaccine has good popularization prospects.

The invention belongs to the technical field of veterinary biological products and particularly relates to a preparation method of a trivalent inactivated vaccine for avian influenza virus, aviadenovirus and avian reovirus. The trivalent inactivated vaccine for avian influenza virus subtype H9, an aviadenovirus type 4 and avian reovirus type 2 is produced by adopting a virus high-adaptability continuous cell line LMH cell. The produced trivalent inactivated vaccine for the avian influenza virus subtype H9, the aviadenovirus type 4 and the avian reovirus type 2 is used for immunizing SPF (Specific Pathogen Free) chickens, so that a high-level HI (Hemagglutination Inhibiting) antibody for resisting the avian influenza virus subtype H9 and a high-level neutralizing antibody for resisting the aviadenovirus type 4 and the avian reovirus type 2 can be generated. The trivalent inactivated vaccine provided by the invention has high titer and complete immune protecting effect on the avian influenza virus subtype H9, the aviadenovirus type 4 and the avian reovirus type 2.

This invention relates to novel unique strains avian reoviridae that were isolated from severe cases of Runting Stunting Syndrome. The present invention also relates to the isolation and uses of novel unique avian reoviridae, diagnostic assays using nucleotide or amino acid specific components of such viruses, such as the sequence encoding Sigma C capsid protein and to vaccines that protect birds from diseases caused by such viruses.

The invention provides a production method for avian reovirus by using a mammalian cell line. The production method comprises the following steps: using a mammalian cell line like a Vero cell line or an MDCK cell line which has formed a cell monolayer with good growth, inoculating avian reovirus which undergoes subculture by using avian kidney cells and allowing avian reovirus to be adsorbed on the cell line; and culturing avian reovirus in a cell maintenance medium instead so as to realize propagation of avian reovirus and harvesting the avian reovirus. An inactivator is added into the avian reovirus produced by using the method, then a vaccine adjuvant acceptable in veterinary pharmacy is added after inactivation, and finally, emulsification is carried out so as to obtain an avian reovirus vaccine.

The invention provides application of a perylenequinone compound in the preparation of feed for preventing and treating poultry viruses, and belongs to the technical field of biological engineering and agriculture. The invention relates to the preparation of the feed for preventing and treating the poultry viruses such as Marek's disease virus, avian leukosis virus subgroup J, chicken infectious anemia virus, avian reovirus, newcastle disease virus, infectious bursal disease virus, goose parvovirus, duck plague virus, avian influenza virus and the like by adding the perylenequinone compound into poultry feed. The perylenequinone compound comprises hypocrellin, elsinochrome, polyanticin, cladosporin, calphostin, cercosporin and the like, and has a series advantages of being convenient to extract, having good stability, having no obvious side effect for oral administration, realizing quick metabolism and the like. The dosage of the perylenequinone compound in the preparation of the feedfor preventing and treating the poultry viruses is 1-50umol of perylenequinone compound per kg by weight every day; the dosage of the perylenequinone compound in the preparation of the feed for preventing and treating the poultry viruses is 50umol-200umol of perylenequinone compound per kg by weight; moreover, the perylenequinone compound has very obvious effect of preventing and treating the poultry viruses, meanwhile can be produced through modern biotechnology, and can bring good economic and social benefits.

The invention discloses an avian reovirus TaqMan MGB probe real-time fluorescent quantitative PCR (polymerase chain reaction) detection kit and application thereof. The invention provides a primer probe set which is composed of a primer 1, a primer 2 and a probe. The nucleotide sequence of the primer 1 is sequence 1; the nucleotide sequence of the primer 2 is a sequence 2; and the nucleotide sequence of the probe is sequence 3. A TaqMan MGB probe technology is utilized to establish a real-time fluorescent quantitative PCR technology for specifically detecting the ARV virus, and the real-time fluorescent quantitative PCR technology is successfully applied to rapid detection and diagnosis of the ARV virus.

The present invention provides a mutant chicken interleukin-1β protein as a chicken interleukin-1β antagonist, which has a substituted peptide at position 117 and/or 118 of wild-type chicken interleukin-1β peptides. The mutant chicken interleukin-1β protein is created by using point mutation in a method genetic engineering; it can significantly inhibit proliferation of avian virus, such as avian influenza virus, avian reovirus and Marek's disease virus, and avian inflammation response. Therefore, the mutant chicken interleukin-1β protein of the present invention can be used as antiviral and anti-inflammatory medication.

The invention provides an avian reovirus virus strain and application, and relates to the field of virus strain screening. The preservation number of the virus strain is CCTCC NO:V201971. According tothe avian reovirus virus strain and application, through isolation and identification, animal regression and challenge protection tests, it is found that the virus strain is a strong virus strain, and existing commercial vaccines or vaccines prepared from clinically isolated virus strains cannot produce effective protection or the protection rate is low, and the virus strain can be used for testing the efficacy of avian reovirus vaccine.

The invention provides a method for culturing an avian reovirus. According to the method, because a vero cell which has excellent performance and is conducive to the proliferation of the avian reovirus is used as a host cell to be used for proliferating the avian reovirus, an optimized process for culturing the avian reovirus can be determined. A bioreactor is adopted to culture and replicate the avian reovirus, and due to the adhesion of the cell to a carrier, the suspension culture of the cell can be realized, the number of vero cells in unit volume can be increased, and further, the yield of the avian reovirus can be improved. Meanwhile, because the avian reovirus culturing process can be monitored timely and controlled quantitatively, the stability of the production process can be guaranteed. Moreover, because the content of avian viral arthritis viruses in an avian viral arthritis virus liquor cultured by adopting the method is high, the content of antigens in the prepared inactivated vaccine is high, and the effect of the inactivated vaccine is good. Furthermore, due to the way of proliferating the avian reovirus by using the bioreactor through the microcarrier, the cost can be lowered, the labor can be saved, and the stability of the product can be improved.