Hybridoma cell strain capable of secreting monoclonal antibodies to quinolones and application of monoclonal antibodies thereof

A technology of hybridoma cell lines and quinolones, applied in the direction of microorganisms, analytical materials, biochemical equipment and methods, etc., to achieve the effect of strong specificity and high sensitivity

Inactive Publication Date: 2012-08-01
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are no immunological kits and test strips for detecting quinolone drug residues at home and abroad. The patent application of the invention uses the principle of competitive inhibition ELISA, and uses the monoclonal antibody of quinolone drugs as the core to establish a competitive ELISA method for detecting quinolone drug residues

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  • Hybridoma cell strain capable of secreting monoclonal antibodies to quinolones and application of monoclonal antibodies thereof
  • Hybridoma cell strain capable of secreting monoclonal antibodies to quinolones and application of monoclonal antibodies thereof
  • Hybridoma cell strain capable of secreting monoclonal antibodies to quinolones and application of monoclonal antibodies thereof

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Embodiment Construction

[0014] Hybridoma cells secreting monoclonal antibodies against fluoroquinolones were deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on November 28, 2011, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, The preservation number is CGMCC No.5608, and the classification is named: secreting fluoroquinolone-resistant monoclonal antibody hybridoma cells;

[0015] Hybridoma cell line secreting anti-quinolone monoclonal antibody ascites indirect ELISA titer up to 10 -7 Above, the antibody type and subclass are IgG1, kappa chain, the monoclonal antibody can be combined with quinolones ciprofloxacin, enrofloxacin, ofloxacin, danofloxacin, norfloxacin, enoxa Star, marbofloxacin, sarafloxacin and difloxacin have specific immune reactions.

[0016] The application of monoclonal antibodies against quinolones in the detection of such antibiotic residues in food is based on various immunological detection met...

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Abstract

The invention discloses a hybridoma cell strain capable of secreting monoclonal antibodies to quinolones and application of the monoclonal antibodies thereof. Ciprofloxacin (CIP) coupled with bovine serum albumin is used as an antigen to immunize BALB/c mice and cell fusion, screening and cloning are carried out so as to obtain one hybridoma cell strain 1F1 capable of stable passage and secretion of monoclonal antibodies (MAb) to quinolones, wherein, the accession number of the hybridoma cell strain 1F1 is CGMCC No. 5608. The titres of ascitic fluids of the 1F1 monoclonal antibodies are up to 10<-7>, and the type and the subclass of the monoclonal antibodies are IgG1 and kappa chain. According to indirect competitive ELISA analysis, the 1F1 monoclonal antibodies perform specific reactions to quinolones like ciprofloxacin, enrofloxacin, ofloxacin, danofloxacin, norfloxacin, enoxacin, marbofloxacin, sarafloxacin and difloxacin. An ELISA method, a kit and test paper for detecting residual of quinolones in food are developed by using the 1F1 monoclonal antibodies.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a hybridoma cell line secreting anti-quinolone monoclonal antibody and the application of the monoclonal antibody. Background technique [0002] Fluoroquinolones (Fluoroquinolones, FQs) are a class of synthetic broad-spectrum, highly effective antibacterial drugs. As a veterinary drug and feed additive, it is widely used in animals, resulting in its residues in animal edible products. Such residues can directly cause harm to the human body: causing cartilage toxicity, joint pain and swelling, skin allergic reactions, leukocyte and thrombocytopenia, eosinophilia, hemolysis, liver and kidney damage, etc. What's more serious is that low concentration of residual drugs may produce drug resistance to FQs drug-sensitive pathogenic bacteria, thus indirectly threatening human health. For this reason, many countries have set strict limit standards for quinolone antibiotics. The maximum resi...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/44G01N33/577
Inventor 吴建祥
Owner ZHEJIANG UNIV
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