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Marine bacillus with multiple functions on plants and its application

A marine bacillus, functional technology, applied in the application, plant growth regulator, plant growth regulator and other directions, can solve the problems of limitation, difficult to survive, colonization, poor soil-borne disease control effect, etc., and achieves low production cost, The biological characteristics and pesticide activity are stable, and the effect of promoting the induction of plant salt resistance

Inactive Publication Date: 2018-01-19
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the development of microbial pesticides at home and abroad, because the biocontrol bacteria are derived from land, it is difficult to survive and colonize in soil with high salinity, and the control effect of soil-borne diseases in saline soil is poor; , the lower ambient temperature limits its function

Method used

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  • Marine bacillus with multiple functions on plants and its application
  • Marine bacillus with multiple functions on plants and its application
  • Marine bacillus with multiple functions on plants and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Artificial cultivation and fermentation of BY-1188 bacterial strain

[0019] The preservation number is: BY-1188 strain of CGMCC No.6187, after the medium optimization test, its suitable formula for culturing and fermenting liquid is: peptone 2-5g, yeast powder 1-3g, NaCl, 25-30g, KCl0. 3-0.5g, MgCl 2 ·7H 2 O1.9-2.5g, FePO 4 0.01-0.02g, 1000ml of water, pH7.0-7.5, the above is the culture solution, add 16-19g of agar to make the solid medium.

[0020] Cultured on the above-mentioned solid medium, and its physical and chemical characteristics were tested by conventional methods. The characteristics of the colony and thallus of the strain: the surface of the colony is rough and opaque, grayish white in the early stage, and then turns into turbid red, with notched edges and many folds on the surface; the thallus is Straight rod, Gram stain positive, 0.5-2.5μm×1.2-10μm, no capsule, perinatal flagella, spores are not enlarged, arranged in pairs or chains;

...

Embodiment 2

[0023] Embodiment 2: the antibacterial activity of BY-1188 bacterial strain to various phytopathogenic fungi;

[0024] The antibacterial activity of the fermentation broth adopts three combined antibacterial test methods (confrontation culture method, 96-well plate method, and antibacterial rate statistical method) to test the antibacterial activity of the bacterial strain. This joint test method can effectively avoid human error and Strains are false positive, and the obtained strains are more practical.

[0025]Among them, confrontation culture method and bacteriostatic rate statistics method are routine methods, which are common to agriculture and seriously harmful to 9 kinds of plant pathogenic fungi: Pyricularia oryzae, Alternaria salani, Fusarium oxysporrum, Botrytis cinerea Cinerea, Verticillium dahliae, Cladosporium fulvum, Phytophthora capsici, Rhizoctonia solani Fusarium graminearum were tested for antibacterial activity;

[0026] The 96-well plate method is based o...

Embodiment 3

[0039] Example 3: Colonization of BY-1188 bacterial strains in living plants

[0040] The methods tested are all conventional test methods for plant colonization: after BY-1188 is marked with rifampicin, the method in Example 1 is cultivated and fermented, and the plant seedlings tested are immersed in the fermentation broth, and are respectively planted in common cultivated soil and seaside saline soil (NaCl content of 0.5%), grind the seedling tissues on 1, 3, 5, 7, 10, and 15 days respectively, take samples and culture them on the plate culture medium added with rifamp, and convert them into bacterial colonies per gram of the sample The number (cfu / g), the results are shown in Table 2. The BY-1188 strain can be planted normally in cucumbers, tomatoes, peppers, cabbage, melons, and eggplants no matter in ordinary cultivated soil or seaside saline soil. After 5 days, the highest colonization numbers of roots, stems and leaves can reach 5.98×10 3 , 6.24×10 3 , 3.78×10 3 , t...

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Abstract

The invention discloses a marine bacillus having multiple functions on plants. The marine bacillus BY-1188 strain (Bacillus marinus) is obtained from a marine habitat, and through artificial mutation breeding and optimization of culture conditions, its preservation number is: CGMCC No. The BY-1188 strain of 6187, the strain has been tested by medium optimization, and its suitable fermentation medium formula is: peptone 2-5g, yeast powder 1-3g, NaCl, 25-30g, KCl0.3-0.5g, MgCl2 7H2O1.9‑2.5g, FePO4O.01‑0.02g, water 1000ml, pH7.0‑7.5, the above is culture solution, add agar 16‑19g to make solid medium. The invention has the advantages of stable biological characteristics and pesticide activity, and no pollution to the environment.

Description

technical field [0001] The invention belongs to the field of marine microbes, specifically bacillus obtained from marine habitats, species name: Bacillus marinus (Bacillus marinus), through artificial mutation breeding and cultivation and fermentation, live bacterial preparations have the functions of disease prevention and promotion of cultivated plants. Growth, induction of cold resistance and salt resistance. The strain is preserved in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures. The address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, and the preservation date is June 5, 2012. No. CGMCC No.6187, classification name Bacillus marinus. Background technique [0002] The safety of agricultural products is the source of all food safety. The development and use of high-efficiency and low-toxicity biopesticides are the basic measures to ensure t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
Inventor 田黎
Owner QINGDAO UNIV OF SCI & TECH
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