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Application of serum 4 type haemophilus parasuis vaccine strain

A technology of Haemophilus suis and Haemophilus suis disease, applied in the application field of serotype 4 Haemophilus parasuis strain, can solve the problems of large difference in virulence and pathogenicity, low immune cross-protection, etc., and achieve Long duration, good protective effect, morbidity and mortality reduction effect

Active Publication Date: 2013-07-03
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many HPs serotypes, and the virulence and pathogenicity of different serotype strains are quite different, and the immune cross-protection between each serotype is very low. Therefore, the existing commercial Haemophilus parasuis vaccine at home and abroad Neither provides complete protection against the disease

Method used

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  • Application of serum 4 type haemophilus parasuis vaccine strain
  • Application of serum 4 type haemophilus parasuis vaccine strain
  • Application of serum 4 type haemophilus parasuis vaccine strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Isolation, purification and identification of serotype 4 Haemophilus parasuis FS0307 strain.

[0016] 1. Culture medium for production

[0017] Preparation of TSA solid medium: Accurately weigh 40g of TSA powder, dissolve in 940mL of distilled water, shake well, and then sterilize by high-pressure steam at 121°C for 15min. 0.01% NAD of bacteria, mixed evenly and poured out for later use.

[0018] Preparation of TSB liquid medium: Accurately weigh 30g of TSB powder, dissolve in 940mL of distilled water, shake well, and then sterilize by high-pressure steam at 121°C for 15 minutes. That's it.

[0019] 2. Isolation, purification and identification of Haemophilus parasuis

[0020] 2.1 Separation and purification

[0021] In 2003, a large number of sick pigs suspected to be infected by Haemophilus parasuis appeared in a large-scale pig farm in Hebei, my country. They were characterized by persistent fever, pleurisy, peritonitis, and arthritis. The lungs, pleur...

Embodiment 2

[0045] Example 2: Application of serotype 4 Haemophilus parasuis FS0307 strain in Haemophilus parasuis disease vaccine.

[0046] 1 strain

[0047] The strain used for seedling production is Haemophilus parasuis FS0307 serotype 4, and its preservation number is CCTCC NO: M2013094. Strong virulence and good immunogenicity.

[0048] 2 Preparation of strains for production

[0049] 2.1 First-level production seed culture Take the lyophilized material of the basic seed of Bacillus parasuis type 4 and dissolve it, inoculate it on TSA / NAD solid medium, cultivate it at 37°C for 24 hours, select more than 5 typical colonies for each plant, and inoculate them on In TSB / NAD liquid medium, shake at 180r / min at 37°C for 18-24 hours. Then the culture is harvested, and after passing the pure inspection, it is used as the primary production seed and stored at 2-8°C for no more than 2 days.

[0050] 2.2 Propagation of secondary production seeds Take primary production seed cultures, inocul...

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Abstract

The invention relates to the field of haemophilus parasuis vaccine strains in veterinarian biological products and discloses an application of a serum 4 type haemophilus parasuis strain. The serum 4 type haemophilus parasuis strain is classified and named as haemophilus parasuis with the strain number of FS0307 and is preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO:M2013094 and the preservation date of March 21, 2013. The serum 4 type haemophilus parasuis strain FS0307 has strong pathogenicity to pigs and good immunogenicity; an inactivated vaccine prepared by applying the serum 4 type haemophilus parasuis strain is safe and reliable and has a good protection effect to pigs attacked by toxicity of homogenous strains; and morbidity and mortality of the pigs after immunization are obviously reduced. Whether a single vaccine or a combined vaccine, immunity of the serum 4 type haemophilus parasuis strain achieves or is better than that of the existing commercialization vaccine on the market, and prevalence of haemophilus parasuis can be effectively prevented.

Description

technical field [0001] The invention relates to the field of Haemophilus parasuis disease vaccine in veterinary biological products, in particular to the application of a serotype 4 Haemophilus parasuis strain. Background technique [0002] Haemophilus parasuis (Haemophilus parasuis, HPs) is a Gram-negative coccus bacillus in the genus Haemophilus of Pasteurellaceae. Porcine Glasser's disease characterized by polyserositis, arthritis and meningitis. It mainly harms piglets aged 2 to 8 weeks, the incidence rate is generally 10% to 15%, and the mortality rate can reach more than 50%. [0003] Haemophilus parasuis has strict nutritional requirements and needs factor V (NAD) strictly for growth. At present, it is considered that tryptone soy agar (TSA) medium and tryptone soy (TSB) medium are the most suitable culture medium for culturing Haemophilus parasuis base. On the TSA plate medium supplemented with NAD, cultured for 24 hours, gray-white colonies with the size of pinpo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/102A61P31/04
Inventor 邵国青刘茂军周勇岐
Owner JIANGSU ACAD OF AGRI SCI
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