A Deodorizing and Deodorizing Strain qdn01 and Its Application in Biological Deodorization

A deodorizing bacteria agent and deamination technology, which is applied in deamination and deodorization bacteria, harmless biological treatment, and deamination and deodorization of poultry manure. It can solve the problems of long cycle time and achieve stable biological characteristics. , Strain reproduction ability is fast, and the effect of deamination and deodorization ability is good

Active Publication Date: 2016-08-24
QIQIHAR UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The deodorization efficiency of this method is higher, but the cycle is longer

Method used

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  • A Deodorizing and Deodorizing Strain qdn01 and Its Application in Biological Deodorization
  • A Deodorizing and Deodorizing Strain qdn01 and Its Application in Biological Deodorization
  • A Deodorizing and Deodorizing Strain qdn01 and Its Application in Biological Deodorization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Isolation, acclimatization and identification of deamination and deodorization bacterial strain QDN01

[0062] 1. Materials and methods

[0063] 1.1 Medium

[0064] Ammonia enriched medium (N1 selective medium): 36.00g sucrose, KH 2 PO 4 2.00g, MgSO 4 0.50g, NaCl2.00g, ammonia water 10.00ml, FeSO 4 0.10g, 1% ZnSO 4 5.0ml, 20g agar, 1000ml distilled water, pH=7.0, sterilized at 121°C for 20min, C / N ratio 7:1.

[0065] Liquid NH 3 Selective medium (N2 medium): N1 medium does not contain agar, C / N is 7:1.

[0066] Denitrification medium (DM medium): C 6 h 5 Na 3 o 7 2H 2 O3.00g, KNO 3 0.7210g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O1.0g, distilled water to 1000ml, adjusted to PH=7.0, sterilized at 121°C for 20min.

[0067] Heterotrophic nitrification medium: C 6 h 5 Na 3 o 7 2H 2 O5.0g, (NH 4 ) 2 SO 4 0.45g, K 2 HPO 4 0.25g, FeSO 4 ·7H 2 O0.0025g, NaCl0.125g, MgSO 4 0.06g, MnSO 4 ·H 2 O 0.001g, dilute to 1000ml with distilled water, adjust...

Embodiment 2

[0114] Example 2 Application of deamination and deodorization bacterial strain QDN01 in deamination and deodorization of chicken manure

[0115] 1. Materials and equipment

[0116] 1.1 Materials

[0117] Fresh chicken manure, QDN01 strain

[0118] 1.2 Drugs and equipment

[0119] Drugs: glucose, concentrated sulfuric acid, hydrochloric acid, sodium hydroxide, 95% ethanol, mercury iodide, potassium iodide, sucrose, normal saline, sodium dihydrogen phosphate, disodium hydrogen phosphate, soluble starch, ammonia water, magnesium sulfate, iron sulfate, sulfuric acid Zinc;

[0120] Equipment: SHZ-D(III) circulating water vacuum pump, electronic balance, PB-10pH, THA-82A desktop constant temperature oscillator, electric heating constant temperature water bath, glass instrument airflow dryer, electric heating constant temperature blast drying oven, TU-1810 UV-Vis Spectrophotometer, Sterilizer

[0121] 1.3 Instruments

[0122] Test tubes, colorimetric tubes, cuvettes, Erlenmeyer...

Embodiment 3

[0193] The application effect of embodiment 3QDN01 bacterial strain in breeding chicken coop

[0194] 1. Method

[0195] (1) Based on chicken manure, starch is used to adjust C / N to 25:1;

[0196] (2) Take 2ml of the seed solution of the deaminated and deodorized bacterial strain QDN01 and transfer it to 98ml of N2 liquid medium with a C / N ratio of 25:1:, shake and cultivate at 130r / min for 24h, centrifuge at 300r / min, and use sterile washed with water, and prepared into 1.0 x 10 12 cells / ml cell suspension;

[0197] (3) Adsorb the cell suspension obtained in step (2) on bentonite to make 1.0×10 12 Individual (bacteria) / g bacterial preparation, add 1% commercially available brown sugar by mass ratio simultaneously, obtain deodorizing bacterial preparation;

[0198] (4) Sprinkle the deodorant bacterial agent of step (3) evenly on the excretion of chicken manure under the breeding cage after step (1), and the usage amount is 200g bacterial agent / m 3 Chicken manure starch wa...

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Abstract

The invention discloses a deammonization and deodorization bacterial strain QDN01 and its application in biological deodorization. The present invention screens a deammonization and deodorization bacterial strain from grass-root black soil, and studies its biological characteristics and denitrification of chicken manure. Through the analysis of physiological and biochemical characteristics and 16SrDNA sequence, the deaminating and deodorizing bacterial strain was identified as Enterobacter hallii, and it was preserved in the China Center for Type Culture Collection with the preservation number CCTCC NO: M2013670. Studies have shown that the QDN01 strain isolated by the present invention has stable biological characteristics and fast reproductive ability, especially can grow rapidly in chicken manure added with starch, and the growth amount reaches 250 billion cfu / ml, and can denitrify well at the same time Deodorization has obvious advantages compared with the prior art. Therefore, the proposal of the present invention provides a very feasible and practical technical solution for the harmless biological treatment of poultry manure, and has a good development prospect.

Description

technical field [0001] The invention relates to a deammonization and deodorization bacterium, and also relates to the application of the strain in the harmless biological treatment of poultry manure and industrial waste water, especially in the deamination and deodorization of poultry manure. The invention belongs to the technical field of biological deodorization. Background technique [0002] With the rapid development of global industrial and agricultural products and the continuous improvement of human living standards, odors also come from the generated waste. Odor pollution is a kind of perceived pollution. Its harmful gases directly affect people's life and health. Therefore, odor pollution has been recognized as one of the six major public hazards after noise pollution. There are many ways to remove odor, and microbial odor removal has become one of the methods with high efficiency, environmental protection and low consumption, and has exerted great potential in env...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F11/02C12R1/01
Inventor 王志刚徐伟慧吴浩玮田险峰赵晓松苏云鹏由义敏
Owner QIQIHAR UNIVERSITY
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