Mycoplasma synoviae

A technology of mycoplasma synovialum and vaccine, which is applied in the field of vaccines, can solve the problems of drug resistance, ineffective effect, and increased control, and achieve good immunogenicity, cross-protection, and reduced morbidity

Pending Publication Date: 2016-07-06
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, the clinical prevention of Mycoplasma synovialis infection in chickens is mainly the administration of appropriate drugs, which play a certain role in preventing the disease, but for those with typical clinical symptoms, the effect of applying drugs is not obvious, and it cannot eradicate the infection in chickens. Mycoplasma gallinarum is prone to drug resistance, making it more difficult to control the disease
It is one of the effective ways to prevent and treat this disease by preparing vaccines with Mycoplasma gallinarum strains. At present, there is no Mycoplasma gallinarum vaccine on the market in China.

Method used

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Examples

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Embodiment 1

[0010] Example 1: Separation, purification and identification of Mycoplasma gallinarum YBF-MS1 strain.

[0011] 1 Culture medium for the production of Mycoplasma synovialis

[0012] 1.1 Preparation of improved CM liquid medium Take 21.0g of PPLO broth, 5g of glucose, add to 1000ml of deionized water, and 1ml of 1% phenol red solution. Add 200ml of inactivated horse serum, 200ml of 25% yeast extract, 1.2ml of 800,000 units / ml penicillin solution, 30ml of 1% coenzyme I solution, 30ml of 1% L-cysteine ​​solution, and use 1mol / L sodium hydroxide Adjust the pH value of the solution to 7.6-7.8, and store at 2-8°C.

[0013] 1.2 Preparation of improved CM solid medium Take 21.0g of PPLO broth, 5g of glucose, 15.0g of agar powder and add deionized water to 1000ml, mix the above ingredients and heat to dissolve, quantitatively dispense, sterilize at 116°C for 30 minutes, put in 2 Store at ~8°C. Heat and dissolve 100ml of solid medium before use. When the temperature drops to about 60...

Embodiment 2

[0042] Embodiment 2: the application of YBF-MS1 strain in chicken mycoplasma synovial bursa vaccine

[0043] 1. The bacterial strain used for making seedlings is Mycoplasma gallinarum YBF-MS1 strain. The thalline, colony morphology and biochemical specificity of the bacterial strain of this invention are all in line with the biological characteristics of Mycoplasma gallinarum. Strong virulence and good immunogenicity.

[0044] 2 Preparation of strains for production

[0045] 2.1 First-level seed propagation Reconstitute the basic freeze-dried seeds of Mycoplasma gallinarum YBF-MS1 strain with improved CM liquid medium, inoculate the seed liquid into the liquid medium at 10% respectively, place at 37°C, and shake at 150r / min After 24 to 30 hours (stop culturing when the color of the culture medium changes from scarlet to orange), the culture is harvested, and after passing the pure inspection, it is used as a first-class seed. Store below -15°C for later use.

[0046] 2.2 Pr...

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Abstract

The invention provides mycoplasma synoviae.A preservation number of the mycoplasma synoviae is CCTCC M 2016080.The mycoplasma synoviae has the advantages that a mycoplasma synoviae YBF-MS1 strain is stable in biological characteristics, high in toxicity and excellent in immunogenicity; oil adjuvant inactivated vaccine prepared from the mycoplasma synoviae is safe and reliable, is long in persistent period and has cross protection properties, high-level antibodies can be generated by the oil adjuvant inactivated vaccine, the post-immunization mycoplasma synoviae morbidity can be obviously reduced, and accordingly epidemic mycoplasma synoviae can be effectively prevented; mycoplasma synoviae vaccine is not available on market in China, the mycoplasma synoviae inactivated vaccine prepared from the mycoplasma synoviae YBF-MS1 strain is a key for preventing and controlling diseases, and market gaps can be filled.

Description

technical field [0001] The invention belongs to the technical field of vaccines, and in particular relates to a mycoplasma chicken synovial bursa. Background technique [0002] Mycoplasmasynoviae (Mycoplasmasynoviae, MS), also known as chicken infectious synovitis, is an acute or chronic infectious disease caused by Mycoplasmasynoviae in chickens and turkeys. Infectious synovitis was first reported by Olson et al (1964). The disease mainly affects joint synovial fluid and tendon sheath, which is characterized by swelling of joints, tendon sheaths and soles. Infection of flocks with the disease can result in significant lameness, stunted growth and carcass degradation. Mycoplasma synovialis infection has spread all over the world and is distributed worldwide. It mainly affects commercial broilers, laying hens and breeders. The disease can occur all year round, but it is easy to occur in winter and spring. Mycoplasma synovialum infection is generally seen in chickens aged 4...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/35
CPCA61K39/02A61K2039/521C12N1/205C12R2001/35
Inventor 马爽范根成孙健程增青赵鹏胡潇郭莉莉刘蕾徐保娟王龙
Owner YEBIO BIOENG OF QINGDAO
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