The invention relates to a pasty enteral nutritional preparation containing egg-white-source oligopeptide nanometer particles and a preparation method, and belongs to the field of food nutrient preparations. The enteral nutritional preparation comprises the following components in percentage by mass: 12-20% of egg-white-source oligopeptide nanometer particles, 20-30% of soybean protein, 40-60% ofmaltodextrin, 12-20% of medium chain triglyceride, 2-3% of corn germ oil, 10-15% of fruit and vegetable powder, 2-3% of prebiotics, 1-3% of arginine and 0.2-2% of complex vitamins. According to the preparation method, the chitosan is mainly used for entrapping egg-white-source oligopeptide to form nanometer particles, then the nanometer particles are mixed with water-soluble components and liposoluble components for many times, and uniform stirring is performed, so that the pasty enteral nutritional preparation is prepared. The enteral nutritional preparation is comprehensive in nutrients, particularly contains medium-chain fatty acids, arginine, various prebiotics and the like; the pasty enteral nutritional preparation has obvious shear thinning effects, and is suitable for postoperativeeating of patients suffering from gastrointestinal tract diseases. According to the pasty enteral nutritional preparation, the digestive absorption burden of stomachs and intestines can be reduced, the postoperative oxidative stress of patients can be notably relieved, the immunity can be improved, and the postoperative restoration of patients can be accelerated.