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Bacillus marinus capable of inducing disease resistance and stress tolerance of plant

A technology of marine bacillus and induced plants, applied in the direction of plant growth regulators, microorganism-based methods, botany equipment and methods, etc., can solve the problems of poor control effect of soil-borne diseases, difficulty in survival, colonization, restrictions, etc., Achieve the effects of stable biological characteristics and pesticide activity, good development prospects, and low production costs

Inactive Publication Date: 2015-11-04
ZHEJIANG FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the development of microbial pesticides at home and abroad, because the biocontrol bacteria are derived from land, it is difficult to survive and colonize in soil with high salinity, and the control effect of soil-borne diseases in saline soil is poor; , the lower ambient temperature limits its function

Method used

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  • Bacillus marinus capable of inducing disease resistance and stress tolerance of plant
  • Bacillus marinus capable of inducing disease resistance and stress tolerance of plant
  • Bacillus marinus capable of inducing disease resistance and stress tolerance of plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Artificial cultivation and fermentation of CT-2628 bacterial strain

[0016] The preservation number is: CT-2628 strain of CGMCC No.8921, through medium optimization test, beef extract 1-3g, peptone 2-3g, yeast powder 1-3g, glucose 1-3g, NaCl, 20-30g, KCl0 .3-0.5g, MgCl 2 ·7H 2 O1.9-2.5g, FePO 4 0.01-0.02g, water 1000ml, pH7.0-8.0 The above is the culture medium, add agar 16-19g to make the solid medium;

[0017] Cultivated on the above-mentioned solid medium, and tested its physical and chemical characteristics by conventional methods, the characteristics of the strain are as follows: the characteristics of the strain are that the colony is turbid yellow, and the surface is mostly wrinkled, sometimes smooth, and dark blue pigment is produced on the peptone medium in the later stage; the bacterium is rod-shaped, Positive Gram staining 0.6-1.9μm×2.9-5.9μm, no capsule, perinatal flagella, spores, aggregated into chains, hydrolyzed starch, gelatin, catalas...

Embodiment 2

[0019] Embodiment 2: The in vitro antibacterial activity of CT-2628 bacterial strain to various phytopathogenic fungi:

[0020] The fermentation broth adopts conventional confrontation culture method and antibacterial rate statistical method. The target bacteria are 9 kinds of plant pathogenic fungi that are seriously harmful to agricultural production: Pyricularia oryzae, Alternaria solani, Fusarium oxysporum, ash The antibacterial test results of Botrytis cinerea, Verticillium dahliae, Cladosporium fulvum, Phytophthora capsici, and Rhizoctonia solani Fusarium graminearum are shown as follows:

[0021] Table 1 CT-2628 antibacterial activity to plant pathogenic bacteria

[0022]

[0023] Note: Plant pathogenic bacteria: Po: Pyricularia oryzae; As: A』ternaria salani;

[0024] Fo: Fusarium oxysporum: Bc: Botrytis cinerea;

[0025] Vd: Verticillium dahliae; Cf: Cladosporium fulvum;

[0026]Pc: Phytophthora capsici; Rs: Rhizoctonia solani;...

Embodiment 3

[0031] Example 3: Colonization of CT-2628 bacterial strains in living plants

[0032] The methods tested are all routine test methods for plant colonization: after CT-2628 was labeled with rifampicin, the above methods were cultured and fermented, tomato seedlings and cucumber seedlings were immersed in the fermentation broth, and planted in common cultivated soil and coastal saline soil (NaCl content 0.5 %), grind each tissue of the seedlings at 1, 3, 5, 7, 10, and 15 days respectively, take samples and culture them on the plate culture medium added with rifamp, and convert them into the number of bacterial colonies per gram of the sample (cfu / g), the result proves that the cucumber, tomato, capsicum, cabbage, muskmelon, and eggplant of the CT-2628 strain can be colonized normally no matter in common cultivated soil or seashore saline soil, and roots, stems, The highest colonization number of leaves can reach 6.00×10 3 , 8.30×10 3 , 4.65×10 3 , 15 days after treatment, CT-...

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Abstract

The invention discloses bacillus marinus capable of inducing disease resistance and stress tolerance of a plant. The bacillus marinus CT2628 (Bacillus sp.) is obtained from an ocean ecological environment through artificial induced mutation breeding and culture condition optimization; the proper artificial culture and fermentation condition is as follows: a proper fermentation culture solution of the CT2628 with the collection number of CGMCC No.8921 is prepared according to the following formula: 1-3g of a beef extract, 2-3g of peptone, 1-3g of yeast powder, 1-3g of glucose, 20-25g of NaCl, 0.3-0.5g of KCl, 1.9-2.5g of MgCl2.7H2O, 0.01-0.02g of FePO4 and 1000ml of water and has the pH value of 7.0-8.0. The invention discloses bacillus marinus capable of inducing disease resistance, salt resistance and cold resistance of the plant and application of the bacillus marinus to agriculture.

Description

technical field [0001] The invention belongs to the field of application of marine microorganisms. Specifically, the bacillus CT2628 strain (Bacillus sp.) obtained from marine habitats is artificially induced, bred, cultured and fermented. role. The strain is preserved in the General Microbiology Center (CGMCC) of China Committee for the Collection of Microbial Cultures, the address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, and the preservation date is March 17, 2014. CT2628 strain numbered CGMCC No.8921. Background technique [0002] At present, environmental protection and food safety issues have received widespread attention from central and local governments. Reducing the use of chemical pesticides has important practical significance for both environmental protection and the safety of food source agricultural products. Biological pesticides are characterized by their low toxicity. , good com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 陈杰田黎
Owner ZHEJIANG FORESTRY UNIVERSITY
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