Haemophilus parasuis and application thereof

A technology for Haemophilus suis and Haemophilus suis disease, which is applied to Haemophilus parasuis and its application fields, can solve the problems of weak immunogenicity and low immune cross-protection, and achieve the reduction of morbidity and mortality, Good protection effect and the effect of reducing economic loss

Inactive Publication Date: 2015-05-13
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of Haemophilus parasuis and application thereof, namely the Haemophilus parasuis serotype 5 vaccine strain LX-5 isolated from Laixi, Qingdao, Shandong, thereby solving the problems of Haemophilus parasuis commercialized vaccines. Low immune cross-protection between serotypes, weak immunogenicity, and low immune cross-protection between different regions of the same serotype

Method used

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  • Haemophilus parasuis and application thereof
  • Haemophilus parasuis and application thereof

Examples

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Embodiment 1

[0013] Example 1: Separation, purification and identification of Haemophilus parasuis serotype 5 vaccine strain LX-5.

[0014] 1. Culture medium for production

[0015] Preparation of TSA solid medium: Accurately weigh 4g of TSA powder, dissolve in 100mL of distilled water, shake well, sterilize by high pressure steam at 121°C for 20min, cool to 50-60°C, add 5% mycoplasma-free fetal bovine serum and 0.005% NAD , mix evenly, pour it out, and store it in a 4°C refrigerator for later use.

[0016] Preparation of TSB liquid medium: Accurately weigh 3 g of TSB powder, dissolve in 100 mL of distilled water, shake well, and then sterilize by high-pressure steam at 121°C for 20 minutes. When cooled to 50-60°C, add 5% mycoplasma-free fetal bovine serum and 0.005% NAD , mix well and store in a 4°C refrigerator for later use.

[0017] 2. Isolation, purification and identification of Haemophilus parasuis

[0018] 2.1 Separation and purification

[0019] The strain was isolated from th...

Embodiment 2

[0042] Example 2: Application of HPs LX-5 strain in inactivated vaccine of Haemophilus parasuis.

[0043] 1 strain

[0044] The strain used for seedling production is Haemophilus parasuis serotype 5 LX-5 strain, and its preservation number is CGMCC No.10230. The LX-5 strain conforms to the standard strain morphology of Haemophilus parasuis, has stable ecological and cultural characteristics, strong toxicity to piglets, and good immunogenicity.

[0045] 2 Preparation of strains for production

[0046] 2.1 First-level production seed culture Take the basal seeds of HPs LX-5 strain to resuscitate, inoculate in TSA solid medium supplemented with NAD and serum, culture at 37°C for 24-48 hours, continuously streak for 3 generations, pick smooth, moist, non-ferrous A single colony with transparent color and the size of a needle tip raised in the middle is inoculated in TSB liquid medium containing NAD and serum, cultured with shaking at 37°C and 180rpm for about 12 hours, then the ...

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Abstract

The invention aims to provide haemophilus parasuis and application thereof. The preservation number of haemophilus parasuis is CGMCC No. 10230. The haemophilus parasuis is used for preparing medicines for treating haemophilus parasuis. A haemophilus parasuis serum type 5 vaccine strain LX-5 has a stable biological characteristic, has relatively strong pathogenicity to a piglet, and has good immunogenicity. A propolis inactivated vaccine prepared by applying the haemophilus parasuis is safe and reliable and can generate an antibody in a relatively high level, is long in duration and has a good protective effect on the piglet counteracting toxic substances for homologous strains. The morbidity and the death rate of the immunized swinery are obviously reduced, and the immune effect of the vaccine reaches or is superior to existing commercialized vaccines in the market, so that prevalence and spread of the haemophilus parasuis can be effectively prevented, the economical loss caused by the disease is lowered, and the haemophilus parasuis has a broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of preparation of veterinary biological products, and in particular relates to Haemophilus parasuis and its application. Background technique [0002] Haemophilus parasuis (HPs for short) is an opportunistic pathogen, also known as Glasser's disease, is a common bacterium in the upper respiratory tract of pigs, belonging to Pasteurella family, Haemophilus, can invade the body under certain conditions and cause severe systemic diseases, such as polyserositis and arthritis, characterized by fever, cough, emaciation, dyspnea, lameness, ataxia and hair loss The main clinical symptoms such as coarse disorder. The disease only infects pigs, and pigs from 2 weeks to 4 months are susceptible, usually seen in pigs of 5 to 8 weeks, mainly after weaning and in the nursery stage. This disease was first described by Glasser in 1910 as a Gram-negative bacillus in the serous secretions of pigs with serous fibrinous pleur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/102A61P31/04C12R1/21
CPCA61K39/102A61K2039/521C12N1/205C12R2001/21
Inventor 单虎段笑笑秦志华张洪亮宋杰韩先杰
Owner QINGDAO AGRI UNIV
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