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Serotype 4 haemophilus parasuis (HPs) vaccine strain

A technology of Haemophilus suis and vaccine strains, applied in the direction of bacteria, antibacterial drugs, biochemical equipment and methods, etc., can solve the problems of low immune cross-protection, large difference between virulence and pathogenicity, and achieve good protection Effect, duration, effect on reduction of morbidity and mortality

Active Publication Date: 2013-07-10
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many HPs serotypes, and the virulence and pathogenicity of different serotype strains are quite different, and the immune cross-protection between each serotype is very low. Therefore, the existing commercial Haemophilus parasuis vaccine at home and abroad Neither provides complete protection against the disease

Method used

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  • Serotype 4 haemophilus parasuis (HPs) vaccine strain
  • Serotype 4 haemophilus parasuis (HPs) vaccine strain
  • Serotype 4 haemophilus parasuis (HPs) vaccine strain

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Example 1: Isolation, purification and identification of serotype 4 Haemophilus parasuis FS0307 strain.

[0016] 1. Culture medium for production

[0017] Preparation of TSA solid medium: Accurately weigh 40g of TSA powder, dissolve in 940mL of distilled water, shake well, and then sterilize by high-pressure steam at 121°C for 15min. 0.01% NAD of bacteria, mixed evenly and poured out for later use.

[0018] Preparation of TSB liquid medium: Accurately weigh 30g of TSB powder, dissolve in 940mL of distilled water, shake well, and then sterilize by high-pressure steam at 121°C for 15 minutes. That's it.

[0019] 2. Isolation, purification and identification of Haemophilus parasuis

[0020] 2.1 Separation and purification

[0021] In 2003, a large number of sick pigs suspected to be infected by Haemophilus parasuis appeared in a large-scale pig farm in Hebei, my country. They were characterized by persistent fever, pleurisy, peritonitis, and arthritis. The lungs, pleur...

Embodiment example 2

[0046] Implementation Case 2: Application of serotype 4 Haemophilus parasuis FS0307 strain in Haemophilus parasuis disease vaccine.

[0047] 1 strain

[0048] The strain used for seedling production is Haemophilus parasuis FS0307 serotype 4, and its preservation number is CCTCC No: M2013094. Strong virulence and good immunogenicity.

[0049] 2 Preparation of strains for production

[0050] 2.1 First-level production seed culture Take the lyophilized material of the basic seed of Bacillus parasuis type 4 and dissolve it, inoculate it on TSA / NAD solid medium, cultivate it at 37°C for 24 hours, select more than 5 typical colonies for each plant, and inoculate them on In TSB / NAD liquid medium, shake at 180r / min at 37°C for 18-24 hours. Then the culture is harvested, and after passing the pure inspection, it is used as the primary production seed and stored at 2-8°C for no more than 2 days.

[0051] 2.2 Propagation of secondary production seeds Take primary production seed cult...

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Abstract

The invention relates to the field of vaccines of Haemophilus parasuis (HPs) in veterinary biological products and discloses a serotype 4 HPs vaccine strain. The class name of the vaccine strain is HPs. The strain number is FS0307. The vaccine strain has been collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:M2013094 and collection date being March 21, 2013. The serotype 4 HPs strain FS0307 has stronger pathogenicity toward swine and has good immunogenicity. The inactivated vaccines prepared from the vaccine strain are safe and reliable and have quite good protective effects on the swine challenging homological strains. The morbidity and mortality of the immunized swinery are obviously reduced. Either the single vaccine or the combined vaccine has the immune effects equivalent or superior to the immune effects of the existing commercialized vaccines and can effectively prevent prevalence of HPs.

Description

technical field [0001] The invention relates to the field of Haemophilus parasuis disease vaccine in veterinary biological products, in particular to a serotype 4 Haemophilus parasuis strain. Background technique [0002] Haemophilus parasuis (Haemophilus parasuis, HPs) is a Gram-negative coccus bacillus in the genus Haemophilus of Pasteurellaceae. Porcine Glasser's disease characterized by polyserositis, arthritis and meningitis. It mainly harms piglets aged 2 to 8 weeks, the incidence rate is generally 10% to 15%, and the mortality rate can reach more than 50%. [0003] Haemophilus parasuis has strict nutritional requirements and needs factor V (NAD) strictly for growth. At present, it is considered that tryptone soy agar (TSA) medium and tryptone soy (TSB) medium are the most suitable culture medium for culturing Haemophilus parasuis base. On the TSA plate medium supplemented with NAD, cultured for 24 hours, gray-white colonies with the size of pinpoints can be seen, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/102A61P31/04C12R1/21
Inventor 邵国青刘茂军周勇岐
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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