Mycoplasma columbinum and application thereof

A technology for Mycoplasma pigeonii and Mycoplasma pigeon is applied to Mycoplasma pigeonii and its application field, and can solve the problems of no vaccine for preventing Mycoplasma pigeonum, accelerated transmission speed, longer disease course, etc., and achieves good protection effect, stable biological characteristics, and strong pathogenicity. force effect

Active Publication Date: 2017-12-08
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2. The speed of transmission is accelerated and the course of disease becomes longer
It also brings some difficulties to the treatment
3. Mixed infection is also serious
There is no vaccine against Mycoplasma pigeonii at present, so a new vaccine with safety and good immunogenicity is urgently needed to prevent and treat the disease

Method used

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  • Mycoplasma columbinum and application thereof
  • Mycoplasma columbinum and application thereof
  • Mycoplasma columbinum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the separation and identification of pigeon mycoplasma

[0020] 1.1 Disease data collection

[0021] The disease material came from the lungs of a racing pigeon that died of sinusitis and dyspnea in a large racing pigeon farm in Zhecheng County, Henan Province in April 2017.

[0022] 1.2 Preparation of medium

[0023] PPLO liquid medium: PPLO broth powder 10.5g, glucose 2.5g, yeast powder 2.5g, dissolved in 440mL ultrapure water, sterilized at 115°C for 15min, added MEM medium 5mL, horse serum 50mL, penicillin 80,000 units, Sterile mass fraction 10% arginine 10mL and 1% (w / v) phenol red 500μL. After the culture was sterilized at 115°C for 15 minutes, it was stored at 4°C for later use.

[0024] PPLO solid medium: PPLO liquid medium added with 1.5% (w / v) agar powder. After the culture was sterilized at 115°C for 15 minutes, it was stored at 4°C for later use.

[0025] 1.3 Isolation and culture of mycoplasma

[0026] Take an appropriate amount of disea...

Embodiment 2

[0042] Embodiment 2, the preparation of vaccine, safety and efficacy test

[0043] 2.1 Preparation of vaccine

[0044] Inoculate the Mycoplasma pigeonii strain HNMco1 into the PPLO liquid medium and place it at 37°C, 5% CO 2 Cultivate in an incubator, collect the culture after 3 days, measure the concentration, and adjust the number of bacteria of Mycoplasma pigeonii in the culture to 2×10 7 CFU / mL, add 2mL formaldehyde solution (mass fraction: 37%) for every 1000mL culture, inactivate at 37°C for 12h to prepare the vaccine stock solution; then add aluminum hydroxide adjuvant (Sigma, F5881) equal to the volume of the vaccine stock solution , mixed to make the inactivated vaccine of Mycoplasma pigeonii, the number of bacteria of Mycoplasma pigeonii in the vaccine is about 1×10 7 CFU / mL.

[0045] 2.2 Vaccine safety test:

[0046] Select 20 healthy pigeons aged 45 days. Divided into 4 groups, 5 in each group. Vaccine group 3 groups, each group was subcutaneously injected wi...

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Abstract

The invention discloses mycoplasma columbinum and application thereof. The mycoplasma columbinum is named mycoplasma columbinum HNMcol, the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO: 14300, the preservation date is June 22, 2016, the preservation unit is CGMCC, and the preservation address is Beijing, China. The mycoplasma columbinum HNMcol is separated from adult race pigeons suffering from typical dyspnea and nasosinusitis, has stable biological properties, has stronger pathogenicity on pigeons, is capable of causing symptoms of typical nasosinusitis, dyspnea and death of the pigeons, and has good immunogenicity. A vaccine prepared by utilizing the mycoplasma columbinum HNMcol is safe and reliable and has better protective effect on a chronic respiratory disease of the pigeons.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a strain of Mycoplasma pigeonii and its application. Background technique [0002] Pigeon Mycoplasma (Mycoplasma columbinum, MC) is the pathogen that causes pigeon chronic respiratory tract disease, and this disease develops into viscous secretion with clear water in the nose, mouth opening breathing, unilateral tearing of the eyes, poor breathing, row of green loose stools, and spreads rapidly. feature. In recent years, the incidence of the disease has increased year by year. Especially the public shed (spring shed) is more serious. The course of treatment is long and the mixed infection is serious. The popular characteristics of the disease: 1. Regardless of seasonality. It occurs throughout the year, but it occurs more frequently in early winter and early spring and is more serious in the rainy season. Pigeons of all ages can happen. 2. The speed of transmissio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/35
CPCA61K39/0241A61K2039/521A61K2039/552A61K2039/55505C12N1/20C12N1/205C12R2001/35
Inventor 徐引弟张青娴郎利敏王治方焦文强张立宪李海利朱文豪游一王克领
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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