Composition, reagent kit and method for detecting mycoplasma bovis

Abstract

The invention discloses a composition, reagent kit and method for detecting mycoplasma bovis. Through designing a specific primer and probe of an LppA gene of mycoplasma bovis, precise detection of the mycoplasma bovis is completed. The primer and probe combination can achieve intraspecific conservative of a mycoplasma bovis standard strain and a wild strain and interspecific specificity of the mycoplasma bovis and other pathogenic bacteria. The optimal reaction time, the optimal reaction temperature, the detection specificity, the detection sensibility, the detection repeatability and the detection stability in the detection process are explored, so that the optimal reaction condition is found out, a mycoplasma bovis fast detection method good in specificity, high in sensibility and capable of being stably repeated is established, and is simple to operate, convenient and time-saving, large experimental instrument equipment is not needed, and the fast detection method is especially suitable for staff without experiment foundation to operate.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a composition, a kit and a method for rapid detection of mycoplasma bovis. Background technique [0002] Mycoplasma bovis (M.bovis) belongs to the kingdom of prokaryotes, Firmicutes, Mollicula, Mycoplasma, Mycoplasma, and Mycoplasma. It is a pathogen that mainly infects the respiratory tract of cattle, and it can continuously infect the host. , causing a variety of chronic diseases including bovine pneumonia, such as mastitis, otitis media, reproductive disorders, arthritis, meningitis, and keratoconjunctivitis, etc. These diseases are often called Mycoplasma bovis associated disease (MbAD) . [0003] The current methods for detecting Mycoplasma bovis generally include the following. The culture method is the direct evidence to detect the existence of the pathogen and is the gold standard for detecting M.bovis. Once detected, it can be diagnosed. It can be divided i...

AI Technical Summary

Problems solved by technology

Although the PCR method is fast and can replace the culture method in a certain sense, its high sensitivity also makes the PCR method relatively demanding on the experimental environment and instruments, and there are problems such as high technical requirements, complicated operation, and difficulty in popularization. Grass-roots laboratories are difficult to develop, and the price is relatively high

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