Construction method of sheep mycoplasmal pneumonia bivalent nucleic acid vaccine containing adjuvant gene

Abstract

The invention discloses a construction method of a sheep mycoplasmal pneumonia bivalent nucleic acid vaccine containing an adjuvant gene. The construction method comprises the following specific steps of: step 1, extraction of a Mo / Mmc genome: S1, recovery and culture of a Mo / Mmc Guizhou strain, and S2, extraction of the DNA of the Mo / Mmc Guizhou strain; step 2, amplifying a P113-LppA-IL-2 fusion gene: S1, amplification of a mucosal immunologic adjuvant gene and mycoplasma ovipneumoniae and Mycoplasma mycoides subsp. Capri antigen gene, S2, the amplification of the P113-LppA fusion gene, and S3, the amplification of the P113-LppA-IL-2 fusion gene; and step 3, preparing the sheep mycoplasmal pneumonia bivalent nucleic acid vaccine containing the mucosal immunologic adjuvant gene. According to the construction method disclosed by the invention, the nucleic acid vaccine is developed by selecting two different antigen genes and mucosal immunologic adjuvant genes, so that the problems of a weak immune effect and the like of the sheep mycoplasmal pneumonia DNA vaccine can be effectively solved, and many problems confronted by current vaccine research and development are solved.

Description

technical field [0001] The invention relates to the technical field of vaccine construction, in particular to a construction method of an adjuvant gene-containing mycoplasma pneumonia bivalent nucleic acid vaccine. Background technique [0002] Mycoplasma pneumonia is caused by Mycoplasma mycoides subsp. Capri (Mmc), Mycoplasma ovipneumoniae (Mo) and Mycoplasma capricolum subsp. Capricolum (Mcc). It is a contagious infectious disease prevalent in sheep flocks. Fibrinous pneumonia and pleurisy are its main features, and it is listed as a B-type infectious disease by the World Organization for Animal Health (OIE). In recent years, there have been reports of the occurrence and prevalence of Mycoplasma ovis pneumonia in many provinces and regions with developed sheep raising industry in China. Loss, and the main pathogens of this disease in my country are Mycoplasma ovis pneumoniae and Mycoplasma mycoides goat subspecies. Immunization is still one of the important measures to p...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for constructing a bivalent nucleic acid vaccine containing an adjuvant gene for Mycoplasma pneumoniae, and to develop a nucleic acid vaccine by selecting two different antigen genes and mucosal immune adjuvant genes, which can effectively target the immune effect of Mycoplasma pneumoniae DNA vaccine Weakness and other problems, to solve many problems faced by the current vaccine research and development

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