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Vaccine strain for mycoplasmal pneumonia of swine

A technology for Mycoplasma hyopneumoniae and Mycoplasma hyopneumoniae, which is applied in the directions of microorganisms, bacteria, biochemical equipment and methods, can solve the problems of difficult separation of Mycoplasma hyopneumoniae, high nutritional requirements, slow growth rate, etc. Good originality and the effect of reducing dosage

Inactive Publication Date: 2015-04-15
TECON BIOLOGY CO LTD
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the in vitro culture of Mycoplasma hyopneumoniae has high nutritional requirements and slow growth rate, and it is easily affected by Mycoplasma hyorhinophylla during the isolation process, which makes it difficult to isolate Mycoplasma hyopneumoniae

Method used

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  • Vaccine strain for mycoplasmal pneumonia of swine
  • Vaccine strain for mycoplasmal pneumonia of swine
  • Vaccine strain for mycoplasmal pneumonia of swine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Isolation and identification of Mycoplasma hyopneumoniae CJ strain:

[0022] Preparation of lung tissue suspension: 30 suspected mycoplasma suis pneumonia lesion lung tissues collected from a pig farm in Changji, Xinjiang were soaked in penicillin containing 1000 units per milliliter, and the surface of the lesion was scalded with an ironing board, and the aseptic operation was carried out. Scissors to remove surface tissue, and take 0.5g of lung tissue, cut into pieces and place in a mortar, add 5ml of modified Friis liquid medium to make a lung tissue suspension;

[0023] PCR identification of lung tissue suspension:

[0024] According to the Takara Nucleic Acid Extraction Kit (Takara Code: DV819A) instructions, the nucleic acid of the lung tissue suspension was extracted and identified by PCR and multiplex PCR;

[0025] PCR identification:

[0026] Primer design and synthesis: Mycoplasma hyopneumoniae PCR primers were synthesized according to the primer sequence in...

Embodiment 2

[0080] Embodiment 2: Observation of immune effect of swine mycoplasma pneumonia inactivated vaccine (CJ strain):

[0081]Vaccine: the inactivated vaccine of mycoplasma pneumoniae of the present invention (CJ strain), contrasts the inactivation vaccine of mycoplasma pneumoniae of a certain foreign company production;

[0082] Bacteria used for inspection: Mycoplasma hyopneumoniae lung tissue virus, isolated, identified, kept and supplied by Xinjiang Tiankang Animal Husbandry Biotechnology Co., Ltd.;

[0083] Experimental animals: 3-4 weeks old healthy susceptible piglets, purchased from Xinjiang Tiankang Animal Husbandry Technology Co., Ltd.;

[0084] Experimental design: The experiment was divided into three groups. The first group and the second group of experimental animals were injected intramuscularly with the inactivated vaccine of mycoplasma pneumonia produced by a foreign company and the inactivated vaccine of mycoplasma pneumonia produced by Xinjiang Tiankang (CJ strai...

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Abstract

The invention discloses a vaccine strain for mycoplasmal pneumonia of swine. The vaccine strain for mycoplasmal pneumonia of the swine, the strain number of which is CJ, is named as mycoplasma hyopneumoniae by classification, and preserved in the China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms (CCCCM) with the preservation number of CGMCC No.9909 on November 6th of 2014. The mycoplasma hyopneumoniae CJ strain grows rapidly and is high in content of bacteria during the preparation of an improved friis liquid medium; moreover, the mycoplasma hyopneumoniae has good immunogenicity and can be prepared into a prophylactic veterinary biological product or applied to veterinary drugs.

Description

technical field [0001] The invention relates to a novel vaccine strain of swine mycoplasma pneumonia, which belongs to the technical field of veterinary biology. Background technique [0002] Mycoplasma pneumonia of swine (MPS), also known as swine panting, is a contact, chronic, non-fatal respiratory disease of pigs mainly caused by Mycoplasma hyopneumoniae (Mhp). The main clinical symptoms are cough and wheezing, and the lesions are characterized by "meat-like" or "shrimp-like" consolidation of the apical lobe, heart lobe, middle lobe, and anterior edge of the diaphragm. It is easy to be infected by other pathogenic bacteria (such as Pasteurella multocida, Haemophilus parasuis, Actinobacillus pleuropneumoniae, etc.), which will increase the mortality rate and seriously affect the growth and feed conversion rate of pigs , thus causing huge economic losses to the pig industry. At present, the main prevention and treatment measures for mycoplasma pneumonia in swine are vacc...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61P31/04C12R1/35
CPCC12N1/20C12N1/205C12R2001/35
Inventor 李新苹候凤王钢贺笋李延涛
Owner TECON BIOLOGY CO LTD
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