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Standard liquid for flow-type cytometer

A flow cytometer and standard solution technology, applied in the field of standard solutions, can solve the problems of individual differences in collected samples, inability to manage staining solutions, and inability to manage dyeing systems

Inactive Publication Date: 2003-01-29
SYSMEX CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, materials obtained from organisms such as cells and bacteria have individual differences in sample collection, and it is difficult to provide stable quality
In addition, the risk of infection with pathogenic bacteria and handling such as refrigerated storage are very troublesome and complicated
[0005] Because latex particles are generally impossible to be dyed, it is not possible to manage the dyeing solution
In addition, fluorescent particles combined with fluorescent dyes can manage the optical system, but cannot manage the dyeing system (not only the management of the dyeing solution itself, but also the management of the dispensing amount of the dyeing solution and temperature management, etc. management)

Method used

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  • Standard liquid for flow-type cytometer
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  • Standard liquid for flow-type cytometer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: the mensuration of urine sample assay standard solution

[0050] Prepare the standard solution and staining solution for urine sample measurement with the following composition.

[0051] :

[0052] HIPRESICA SP TM 40 cells / μl

[0053] (average particle diameter 5.1 μm, for red blood cells, manufactured by Ube Nitto Kasei Co., Ltd.)

[0054] Zorbax BP-SIL TM 40 cells / μl

[0055] (Particle size 7-8μm, for white blood cells, manufactured by GL Science Co.Ltd)

[0056] NUCLEOPREP 100-30 TM 40 cells / μl

[0057] (average particle size 30 μm, for epithelial cells, manufactured by MACHEREY-NAGEL)

[0058] Sepharose CL-2B TM 2 / μl

[0059] (Particle size 60-200 μm, for columnar cells, manufactured by Parmacia Biotec Co.Ltd.)

[0060] Pia seed S-150 TM 40 cells / μl

[0061] (average particle size 2μm, for bacteria, manufactured by Pia TEC Co.Ltd)

[0062] Sodium chloride 10g / L

[...

Embodiment 2

[0084] Embodiment 2: the mensuration of urine assay standard solution

[0085] :

[0086] HIPRESICA sp TM 200 cells / μl

[0087] Zorbax BP·SIL TM 200 cells / μl

[0088] NUCLE OPREP 100-30 TM 800 cells / μl

[0089] Bio·GelA·5m(Fine) TM 13 / μl

[0090] (Particle size 38-75 μm, columnar cells, manufactured by Bio-Rad Laboratories)

[0091] Pia Seed S 150 TM 200 cells / μl

[0092] Sodium formate 30.5g / L

[0093] Hydrochloric acid 5.0g / L

[0094] Ethanol (fluorescent sensitivity stabilizer) 9.5w / w%

[0095] NS·80D TM (Isothiazoline preservatives, Nagase Kaseikogyo co., Ltd

[0096] pH4.0 electrical conductivity 25ms / cm

[0097] Same as Example 1, first prepare a concentrated dispersion containing only various particles, measure the number of particles, dilute to the above-mentioned particle concentration and mix to prepare the above-mentioned standard solution.

[0098] The mensuration of standard solution...

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Abstract

This invention relates to a standard fluid for a flow cytometer is characterized by showing, after staining, almost the same fluorescence intensity and scattered light intensity as those of the cells to be assayed. The present invention aims at providing a standard fluid for a flow cytometer which shows almost the same behaviors as those of the cells to be assayed and can be easily handled without any risk of infection.

Description

technical field [0001] The invention relates to a standard solution used in precision control and calibration of flow cytometers. More specifically, it relates to a flow cytometer standard solution for analyzing cells in urine. Background technique [0002] A method using a flow cytometer is known for classifying and counting cells in blood and particles in urine. This method is to stain the cells in the sample sample with the staining solution, make the cells flow one by one in the flow cell, and then irradiate the stained cells with excitation light, and measure the fluorescence and scattered light emitted from the cells. Category count. [0003] However, when performing analysis with a flow cytometer, it is necessary to calibrate the device in advance in order to obtain correct data. Generally, immobilized red blood cells, cells such as bacteria, latex particles, fluorescent particles, etc. are used for precision control and calibration of the flow cytometer. For exam...

Claims

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Application Information

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IPC IPC(8): G01N33/48G01N1/00G01N15/10G01N15/14G01N33/483G01N33/50
CPCY10T436/101666Y10T436/10G01N15/1012G01N15/1459
Inventor 松本辉也福田正和
Owner SYSMEX CORP
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