Separable hydrogel microneedle loaded with recombinant human fibronectin as well as preparation method and application of separable hydrogel microneedle

Abstract

The invention relates to a separable hydrogel microneedle loaded with recombinant human fibronectin and a preparation method and application thereof, the separable hydrogel microneedle loaded with recombinant human fibronectin comprises a microneedle substrate and a microneedle tip separably attached to the microneedle substrate, the microneedle tip is formed by crosslinking and curing a hydrogel precursor solution through UV initiation, and the hydrogel precursor solution comprises a hydrogel material, a carrier delivery protein, a photoinitiator and an aqueous medium; the delivery protein is recombinant human fibronectin obtained through genetic engineering. A hydrogel material, carrier delivery protein, a photoinitiator and an aqueous medium are subjected to UV-induced crosslinking curing to obtain the separable microneedle tip, the microneedle tip can be retained in a body after application, and a carrier drug embedded in the microneedle tip is released to surrounding tissues through diffusion. And retention and slow release of functional drugs are realized.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a separable hydrogel microneedle loaded with recombinant human fibronectin, a preparation method and application thereof. Background technique [0002] Wound healing is a complex process that involves fundamental processes such as inflammation, fibrous proliferation, neovascularization, collagen deposition, epithelialization, and wound contraction. During the healing process, the body secretes various growth factors to accelerate wound healing. The normal healing process occurs at an optimal rate in healthy individuals but is often delayed or even completely impaired in diabetic patients, and poor wound healing is a major clinical problem in diabetes. [0003] Fibronectin (Fn) is a high-molecular-weight glycoprotein that circulates in plasma as a soluble dimer and exists in the extracellular matrix as a cell-derived multimeric insoluble form, whose structure conta...

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Problems solved by technology

The disadvantage of local direct application is that the protein is easily hydrolyzed by proteases on the wound surface, loses its activity, and requires long-term multiple administration; when hydrogel is used as a protein delivery carrier, the protein can be retained in the three-dimensional network of the hydrogel to reduce the interaction with the surrounding tissue. The contact with protease protects the activity of the protein. However, the hydrogel protein delivery patch only releases protein in the superficial tissue, and it is difficult for macromolecules such as protein to diffuse into the tissue at a shallow depth of administration, so the effect is limited.

The application of recombinant human fibronectin in wound treatment is mainly through solution spraying. However, this method is difficult to maintain protein activity for a long time and requires repeated administration, which increases the cost of treatment.

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