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Stem cell composite PLGA (polylactic-co-glycolic acid) support kit and application thereof

A stem cell and kit technology, applied in the field of obstetrics and gynecology, can solve the problems of easy occurrence of apoptosis and unfavorable promotion and application of stem cells, and achieve the effects of reducing the risk of spreading pathogens, having no risk of spreading pathogens, and having a simple preparation process.

Inactive Publication Date: 2019-01-04
京和生殖医学技术(沈阳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the results of in vivo and in vitro experiments show that the PLGA scaffold can provide attachment sites for stem cells and promote the differentiation of stem cells. However, most pluripotent stem cells are prone to apoptosis when grown under in vitro culture conditions without the in vivo microenvironment. It is not conducive to the promotion and application of stem cells

Method used

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  • Stem cell composite PLGA (polylactic-co-glycolic acid) support kit and application thereof
  • Stem cell composite PLGA (polylactic-co-glycolic acid) support kit and application thereof
  • Stem cell composite PLGA (polylactic-co-glycolic acid) support kit and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Prepare a complete medium, then resuscitate and subculture human bone marrow mesenchymal stem cells, and then carry out compound culture of human bone marrow mesenchymal stem cells and PLGA scaffolds to obtain stem cell composite PLGA scaffolds, and then surgically implant to repair the endometrium.

[0034]Step 1, prepare complete medium: add 5ml double antibody, 50ml fetal bovine serum and 5μmol Blebbistatin to 500ml low-sugar DMEM basal medium to make a complete medium containing 10% fetal bovine serum, 1% double antibody, 10μM blebbistatin .

[0035] Step 2, recovery of human bone marrow mesenchymal stem cells: take out the frozen cells, immediately put them in a 37°C water bath, and shake slowly to thaw the cells quickly; then transfer the cell suspension to a 15mL centrifuge tube, add 3 times the volume to complete Mix the culture medium by pipetting with a gun; centrifuge (1000rpm at room temperature, 5min), discard the supernatant; add low-sugar DMEM basal mediu...

Embodiment 2

[0047] Prepare a complete medium, then resuscitate and subculture human bone marrow mesenchymal stem cells, and then carry out compound culture of human bone marrow mesenchymal stem cells and PLGA scaffolds to obtain stem cell composite PLGA scaffolds, and then surgically implant to repair the endometrium.

[0048] Step 1, recovery of human bone marrow mesenchymal stem cells: take out the frozen cells, immediately put them in a 37°C water bath, and shake slowly to thaw the cells quickly; then transfer the cell suspension to a 15mL centrifuge tube, add 3 times the volume of The low-sugar DMEM basal medium of double antibody, fetal bovine serum and Blebbistatin was blown and mixed with a gun; centrifuged (room temperature 1000rpm, 5min), and the supernatant was discarded; the complete medium was added to resuspend the cells, and then inoculated into a culture flask.

[0049] Step 2, subculture of human bone marrow mesenchymal stem cells: change the medium of the above culture med...

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Abstract

The invention belongs to the field of obstetrics and gynecology, and particularly relates to a stem cell composite PLGA (polylactic-co-glycolic acid) support kit for repairing intrauterine adhesion endometrium injury of and an application of the support kit. The kit comprises human bone marrow mesenchymal stem cells with the concentration of 5-7*10<6> pieces / mL and a PLGA support. The kit containsthe PLGA support, the PLGA support can provide attachment sites for the bone marrow mesenchymal stem cells, the number of the stem cells positioned on an injury portion is increased, so that an injured endometrium is repaired, repairing effects are improved, risks caused by transfer of the stem cells are reduced, and the PLGA support can facilitate the stem cells to be differentiated and is madeof synthesized biological materials. Compared with materials from biological collagen and the like, and the kit has the advantages that the kit is wide in source, simple in preparation process, free from pathogen transmission risks and the like.

Description

technical field [0001] The invention belongs to the field of obstetrics and gynecology, and in particular relates to a stem cell composite PLGA scaffold kit for repairing uterine cavity adhesive endometrial damage and an application thereof. Background technique [0002] The endometrium is extremely important for maintaining female physiological characteristics and reproductive function, and plays a key role in embryo implantation and maintenance of pregnancy. The endometrium has a strong ability to regenerate, but it is extremely susceptible to many factors such as miscarriage, infection, endocrine, etc. The receptivity of the endometrium caused by various reasons is significantly reduced, which seriously affects the fertility of women. Endometrial adhesions (IUAs) are common acquired endometrial disorders secondary to endometrial injury such as trauma, curettage, infection, congenital anomalies of the uterus, and genetic predisposition. Its main pathological feature is ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/38A61L27/54
CPCA61L27/18A61L27/3834A61L27/54A61L2300/412C08L67/04
Inventor 王芳项剑桥郑珊珊
Owner 京和生殖医学技术(沈阳)有限公司
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