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Method for efficiently catalyzing TMB (Tetramethylbenzidine) color development reaction by using carbon nanoparticles

A technology of carbon nanoparticles and color reaction, applied in the field of nanometers, can solve the problems that no one has reported about carbon nanoparticles, and achieve the effects of short color development time, good catalytic effect and high catalytic efficiency

Inactive Publication Date: 2012-06-27
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, graphene and single-walled carbon nanotubes have been reported to catalyze chemical reactions, but no one has reported to use carbon nanoparticles to catalyze chemical reactions.

Method used

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  • Method for efficiently catalyzing TMB (Tetramethylbenzidine) color development reaction by using carbon nanoparticles
  • Method for efficiently catalyzing TMB (Tetramethylbenzidine) color development reaction by using carbon nanoparticles
  • Method for efficiently catalyzing TMB (Tetramethylbenzidine) color development reaction by using carbon nanoparticles

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Experimental program
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Effect test

Embodiment 1

[0033] Weigh 13 mg of non-carboxylated carbon nanoparticles, ultrasonically treat them in water with SDS surfactant for one hour, then drop them on silicon wafers, and use SEM to characterize them. 13mg of carboxylated carbon nanoparticles was weighed, ultrasonically treated with SDS surfactant in water for one hour, then dropped on a silicon wafer, and characterized by SEM. Take a certain amount of carbon quantum dots and drop them on a silicon wafer, and use TEM to characterize; figure 1 , the dispersion of carbon nanoparticles is relatively uniform after treatment with surfactant, and the color rendering effect is better.

Embodiment 2

[0035] At room temperature, 250 μM TMB solution (1M concentration) and different concentrations of H 2 o 2 (respectively 0, 0.25, 2.5, 12.5, 25, 250, 500μM) was added to 400μL of water, and then a certain concentration of CNPs-SDS was added, and the absorption value was measured at a wavelength of 652nm as a function of time; figure 2 , when the other amounts remain unchanged, with the increase of hydrogen peroxide concentration, the color development of TMB reaction catalyzed by CNPs-SDS is more obvious, H 2 o2 The preferred addition amount is 500 μM per 400 μL of water.

Embodiment 3

[0037] At room temperature, 250 μM TMB solution (1M concentration) and 250 μM H 2 o 2 Add 400 μL of water, and then add different concentrations of CNPs-SDS (respectively 0, 5, 10, 20 μL), and measure the absorption value over time at a wavelength of 652 nm; image 3 , indicating that in the case of other amounts remaining unchanged, the color of the TMB reaction is significantly catalyzed with the increase of the amount of CNPs-SDS, and the preferred addition amount of CNPs-SDS is 10 μL per 400 μL of water.

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Abstract

The invention belongs to the field of nanometer technologies and particularly relates to a method for efficiently catalyzing a TMB (Tetramethylbenzidine) color development reaction by using carbon nanoparticles. The method comprises the steps of: adding 250 mu M of TMB solution and 0.25-500 mu M of H2O2 into 400 mu L of water, then adding 5-40 mu L of surfactant-modified carbon nanoparticles, shaking to reach a uniform state, and measuring time-varying absorption values under the wavelength of 652nm, wherein the surfactant is anyone of SDS (Sodium Dodecyl Sulfate), DNA (Deoxyribose Nucleic Acid), DTAB (Dodecyl Trimethyl Ammonium Bromide) and Tuwen20, and the dimension of the carbon nanoparticles is 20-70nm. By utilization of the carbon nanoparticles as a catalyst, the favorable characteristics of cheap materials, short color development time, high catalysis efficiency and the like are achieved, and the catalytic effect of the carbon nanoparticles is far better than that of graphene and single-walled carbon nanotubes.

Description

technical field [0001] The invention belongs to the field of nanotechnology, in particular to the catalytic reaction of carbon nanoparticles (Carbon Nanoparticles) in carbon nanomaterials. Background technique [0002] Carbon nanoparticles (CNPs) are a new type of carbon nanomaterials that have recently emerged. It, together with carbon nanotubes (CNTs), graphene and other carbon materials, belongs to the frontier of research in the field of nanomaterials. At present, it has been reported that graphene, carbon nanotubes and other carbon nanomaterials are used to catalyze various chemical reactions. In 2010, Qu et al published a report on the use of single-walled carbon nano tube (SWNTs) catalyzes the color-changing reaction of 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2, mainly because it was found that SWNTs have an activity similar to peroxidase, and the energy dispersive X-ray According to analysis (EDX), this catalytic activity is not caused by the trace ...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/33B01J21/18
Inventor 杨荣华刘金华
Owner HUNAN UNIV
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