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Double-quantum-dot nano complex nitrogen monoxide ratiometric fluorescent probe and preparation method thereof

A technology of ratiometric fluorescent probes and nanocomposites, applied in the field of nanotechnology and chemical analysis, to achieve the effects of good fluorescence stability, low cost, improved accuracy and feasibility

Inactive Publication Date: 2016-08-24
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After reviewing the literature, it is shown that although the work of measuring NO using quantum dot single fluorescence has been reported (L.Tan, A.Wan, H.Li and Q.Lu, Acta Biomater., 2012, 8, 3744-3753; L.Tan ,A.Wan and H.Li,Mater.Chem.Phys.,2013,138,956-962), up to now, there are no domestic and foreign literatures and related patent reports on the compound based on double quantum dots for ratiometric fluorescence detection of NO

Method used

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  • Double-quantum-dot nano complex nitrogen monoxide ratiometric fluorescent probe and preparation method thereof

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Embodiment 1

[0031] The preparation method of double quantum dot nanocomposite NO ratio fluorescent probe (such as figure 1 shown), the detailed preparation steps are as follows: (1) Select CdSe quantum dots stabilized by mercaptopropionic acid, the maximum emission wavelength is 532nm, and the mass concentration is 2mg mL -1(2) Take 5mL CdSe quantum dot water dispersion and 0.1g cetyltrimethylammonium bromide and add to "50mL ethanol + 1mL distilled water + 1mL ammonia water + 0.2mL tetraethyl orthosilicate" Precursor solution, disperse for 10min under the action of ultrasonic waves, and then react for 2h at room temperature under magnetic stirring; (3) CdSe@SiO is obtained by centrifugation 2 The composite, whose shell thickness is controlled to be about 5nm, is dispersed in a mixture of "50mL ethanol + 0.1mL 3-aminopropyltriethoxysilane", and reacted with magnetic stirring at room temperature for 6h to prepare CdSe@SiO 2 -NH 2 Core-shell complex, configured to a mass concentration of ...

Embodiment 2

[0033] The preparation method of the double quantum dot nanocomposite NO ratio fluorescent probe, the detailed preparation steps are as follows: (1) select the CdSe quantum dots stabilized by mercaptopropionic acid, the maximum emission wavelength is 540nm, and the mass concentration is 4mg mL -1 (2) Take 5mL CdSe quantum dot aqueous dispersion and add 0.2g cetyltrimethylammonium bromide to "100mL ethanol + 2mL distilled water + 2mL ammonia water + 0.4mL tetraethyl orthosilicate" Precursor solution, disperse for 10min under the action of ultrasonic waves, and then react for 2h at room temperature under magnetic stirring; (3) CdSe@SiO is obtained by centrifugation 2 The composite, whose shell thickness is controlled to be about 10nm, is dispersed in a mixture of "100mL ethanol + 0.2mL 3-aminopropyltriethoxysilane", and reacted with magnetic stirring at room temperature for 6h to prepare CdSe@SiO 2 -NH 2 Core-shell complex, configured to a mass concentration of 4mg mL -1 Stand...

Embodiment 3

[0035] The preparation method of the double quantum dot nanocomposite NO ratio fluorescent probe, the detailed preparation steps are as follows: (1) select the CdSe quantum dots stabilized by mercaptopropionic acid, the maximum emission wavelength is 525nm, and the mass concentration is configured to be 6mg mL -1 (2) Take 5mL CdSe quantum dot aqueous dispersion and add 0.4g cetyltrimethylammonium bromide to "100mL ethanol + 2mL distilled water + 4mL ammonia water + 0.8mL tetraethyl orthosilicate" Precursor solution, disperse for 10min under the action of ultrasonic waves, and then react for 2h at room temperature under magnetic stirring; (3) CdSe@SiO is obtained by centrifugation 2 The composite, whose shell thickness is controlled to be about 15nm, is dispersed in a mixture of "100mL ethanol + 0.2mL 3-aminopropyltriethoxysilane", and reacted with magnetic stirring at room temperature for 6h to prepare CdSe@SiO 2 -NH 2 Core-shell complex, configured to a mass concentration of...

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Abstract

The invention discloses a double-quantum-dot nano complex nitrogen monoxide ratiometric fluorescent probe and a preparation method thereof, belonging to the fields of nano technology and chemical analysis. The preparation method of the probe comprises the following steps: (1) carrying out reaction on mercaptopropionic-acid-stable CdSe quantum dots and a silane agent to obtain an amino-surface-functionalized CdSe@SiO2 core-shell complex; (2) dropwisely adding the mercaptopropionic-acid-stable CdSe quantum dot dispersion solution into the core-shell complex system to prepare a CdSe@SiO2-CdTe nano complex; and (3) introducing NO into the nano complex system, determining the fluorescence emission spectrum of the system, fitting the linear relationship between the CdTe / CdSe quantum dot ratiometric fluorescence intensity and NO concentration, and establishing the NO ratiometric fluorescent probe based on the CdSe@SiO2-CdTe nano complex. Compared with the prior art, the ratiometric fluorescence intensity introduced into the fluorescent probe can obviously enhance the NO detection accuracy and feasibility, and has important application value.

Description

technical field [0001] The invention relates to the fields of nanotechnology and chemical analysis, in particular to a dual-quantum dot nanocomposite nitric oxide ratio fluorescent probe and a preparation method thereof. Background technique [0002] As a diatomic free radical produced in the human body, nitric oxide (NO) effectively regulates the physiological functions of the cardiovascular, respiratory and nervous systems. NO is also actively involved in the immune response, promoting macrophages to fight foreign pathogens. NO has the characteristics of lively nature, short life, rapid chemical reaction, and easy combination with other substances. As an important biological messenger molecule, NO can rapidly diffuse in cells and tissues, and then react with targets. Based on this, the detection of NO is a very meaningful work, and the development of an efficient NO detection method has also aroused extensive research interests of relevant scholars. [0003] Compared wi...

Claims

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Application Information

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IPC IPC(8): C09K11/88G01N21/64B82Y20/00B82Y40/00
CPCB82Y20/00B82Y40/00C09K11/883G01N21/6428
Inventor 孙捷李娜王晓静王兵孙敬勇吴忠玉刘腾李慧娟李玲秦桂芝徐彩云
Owner UNIV OF JINAN
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