9 results about "Dna double helix" patented technology

A comprehensive teaching aid system for genetic science at least including numerous (deoxy-) ribonucleotide models that can be assembled to form DNA / RNA single strands, or to form the beautiful DNA double helix structure when numerous adjacently and oppositely connected deoxynucleotide models are attached by magnets, tRNAs, and three different plates for DNA replication, mRNA transcription and protein synthesis respectively. The (deoxy-) nucleotide model includes a phosphate model, a (deoxy-) ribose model and a base model connected in sequence. Between two adjacently disposed (deoxy-) ribonucleotide models, a (deoxy-) ribose model is connected to a phosphate model in the head-to-tail fashion to form a detachable and flexible chain structure. The base model is laterally connected to the (deoxy-) ribose model, and two base models in two oppositely disposed deoxynucleotide models are flexibly and complementarily attached.

The invention provides a novel organic synthesis fluorescent probe as well as a preparation method and application thereof, and the method comprises the following steps: carrying out a palladium-catalyzed cross-coupling reaction on 3, 6-dibromo-9-phenylcarbazole and 4-vinylpyridine to generate a compound Ph-BPV-Cz, and introducing iodoethane through a quaternization reaction to obtain the novel organic synthesis fluorescent probe. Key structural units such as carbazole, pyridine and vinyl are ingeniously fused in a molecular skeleton of the probe, so that the probe has excellent optical properties, good chemical stability, rich reaction activity and modifiability. Through the synergistic effect of the structural units, the probe can be specifically combined with RNA, and the obvious change of the fluorescence characteristic of the probe is triggered, so that the probe can be used for RNA detection. Besides, PCz-ViPy-Et-PCz effectively enhances the chimeric ability with a DNA double-helix groove by virtue of the synergistic effect of a rigid carbazole skeleton and a pyridinium group, so that specific recognition and fluorescence response to a DNA sequence are realized, and the probe can be used for DNA detection.

The invention relates to the technical field of sequencing, in particular to a preparation method of a sequencing library and a reagent. The invention provides a preparation method of a sequencing library, which comprises the following steps of: additionally adding an additive or an additive combination capable of reducing a DNA secondary structure and / or reducing single-stranded DNA degradation in single-stranded cyclization reaction and / or rolling circle amplification reaction; an additive or an additive combination capable of reducing a DNA secondary structure, reducing single-stranded DNA degradation and / or reducing double-stranded DNA double-helix stability is additionally added in a double-stranded cyclization library rolling circle amplification reaction, so that the binding capacity of DNA ligase and / or strand displacement DNA polymerase and target nucleic acid is improved, and the situation that the rolling circle amplification efficiency of different circular DNAs is not uniform due to the secondary structure is reduced; the probability that the loose single-stranded DNA is easy to degrade is reduced, and the amplification uniformity of different GC regions and different sequence characteristic regions of the library is improved, so that the sequencing quality and the sequencing data yield are improved.

This invention discloses a three-dimensional micromixer based on the DNA double helix structure, belonging to the field of microfluidics technology. The micromixer has two spatial helical channels with a π-phase difference, periodically coupled through staggered, "base pair"-like connecting channels. The centrifugal force generated by the three-dimensional helical microchannels induces Dean's vortexes, producing a "rotation-revolution" composite effect during fluid flow. Furthermore, the mass transfer between the two channels is enhanced through the splitting-recombination effect of the "base pair" connecting channels. Compared with existing technologies, this invention has the following advantages: 1. It induces complex three-dimensional flow through the three-dimensional DNA double helix structure, realizing continuous renewal and stretching of the fluid interface; 2. It utilizes the curvature and torsion of the three-dimensional helical structure to generate a "revolution" enhanced mixing effect under the superposition effect of rotation and deflection, forming a "rotation-revolution composite flow" similar to planetary motion, overcoming the mixing difficulties caused by low diffusion efficiency and difficulty in forming chaotic convection in high-viscosity oil phase systems; 3. Under the same mixing efficiency, the pressure drop of this device is much smaller than that of traditional two-dimensional planar serpentine and split-and-merge micromixers, enabling rapid and uniform mixing of high-viscosity fluids with low energy consumption, and is particularly suitable for mixing and mass transfer enhancement of high-viscosity oil phase systems in fine chemical processes.

The application provides a plant transcription factor binding site prediction method fusing full dynamic convolution and CubeMLP, DNA sequence data of a plant transcription factor binding site region obtained through sequencing is subjected to One-hot coding processing and DNA double helix spatial structure feature extraction; mode feature of different categories of TFBS sequences is dynamically extracted by using an ODConv network, DNA spatial structure feature is extracted by using a convolutional neural network; efficient fusion of DNA sequence feature mode and DNA spatial structure mode feature is realized from three dimensions of sequence, mode and channel by using CubeMLP; the result of the fused feature is mapped into transcription factor binding sites of different categories by using a full connection layer and a Sigmoid function, and then the recognition of transcription factor binding sites of different categories is realized; the function of predicting transcription factor binding sites is realized. The application has high prediction accuracy, provides a new reference for the research of the recognition site of a plant transcription factor, and has a wide application prospect.

The invention discloses a cascade dual-AND logic DNA nano circuit and a preparation method and application thereof, and the cascade dual-AND logic DNA nano circuit comprises a tubular six-helix frame nucleic acid composed of six DNA double helixes and a drug encapsulated in a tubular cavity of the tubular six-helix frame nucleic acid, the tubular six-helix framework nucleic acid is mainly synthesized by DNA single-stranded hybridization with nucleotide sequences shown as SEQ ID NO: 1-20, and the drug is connected with the tubular six-helix framework nucleic acid through a disulfide bond. The cascade dual-AND logic DNA nanocircuit provided by the invention has a cascade dual-AND logic gate, can sequentially respond to three tumor specific signals of acidic pH, nucleolin and glutathione according to a strict space-time sequence, and can realize accurate release of drugs when being applied to drug delivery, thereby minimizing off-target toxicity and remarkably improving the treatment effect of the drugs.

The invention provides an SNP (Single Nucleotide Polymorphism) detection method and an SNP detection product based on a nanopore contraction region, and belongs to the technical field of gene detection. According to the invention, a nano patch clamp system is constructed, a unique structure of a glass nano pore channel is combined, a double-chain nucleic acid molecule containing an SNP site is guided to a contraction region, and a characteristic ion current change is initiated by utilizing hydrogen bond or electrostatic interaction of a mismatched base and a lysine residue in the pore channel, so that high-sensitivity detection with single base resolution is realized. By means of hydrogen bonds or electrostatic interaction between mismatched bases and lysine residues with positive charges in a nanopore channel contraction region, when nucleic acid molecules containing SNP sites pass through nanopore channels, the mismatched bases can damage the stability of a DNA double-helix structure, conformations of the nucleic acid molecules in the contraction region are changed, and the nucleic acid molecules containing the SNP sites pass through the nanopore channels. Therefore, the path and the efficiency of ions passing through the nanopore are influenced, characteristic current fluctuation is triggered, and the resolution of a single base level is realized.

The application discloses a spiral SiC nanotube electromagnetic wave absorbing material and a preparation method thereof. The absorbing material is in the shape of DNA double helix with two nanotubes intertwined with each other. The spiral SiC nanotube is prepared by taking a spiral polypyrrole nanotube as a precursor and through a heat treatment method. The absorbing material has good stability below 1000 DEG C and excellent high-temperature resistance. The special spiral structure introduces rich interfaces to improve polarization loss. When the matching thickness is 1.4 mm, the absorbing frequency band width with a reflection loss lower than -10 dB can reach 4.2 GHz.

The application discloses a biomimetic DNA double helix structure methane hydrogen mixer, relates to the technical field of hydrogen energy utilization, and can make methane and hydrogen more fully contact and mix in the mixer through a DNA double helix structure formed by two spiral columns and a guide plate, so that the mixing uniformity is improved. Meanwhile, the biomimetic DNA double helix structure can optimize the fluid channel and flow velocity distribution in the mixing element, so that the fluid resistance is reduced, the energy loss in the mixing process is reduced, and the energy efficiency of the whole system is improved.