4-10 decoder design method based on strand displacement

Abstract

The invention provides a 4-10 decoder design method based on strand displacement, and relates to the field of DNA strand displacement. According to the method, firstly concentration of DNA signal chains is set according to the input logic value of a DNA 4-10 decoder, and each DNA signal is divided into 10 different DNA signal chains having the same logic value through a fan-out gate module; then hybridization reaction with an AND gate module and an OR gate module is performed based on the Watson-Crick (C-G, T-A) complementary matching principle according to difference of domains attached on the outputted 80 DNS signal chains; and finally 20 DNS signal chains are outputted, and the 20 DNS signal chains represent different logic values according to the size of concentration so that the function of the 4-10 decoder can be realized. The beneficial effects of the 4-10 decoder design method based on strand displacement are that the AND gate module, the OR gate module and the fan-out gate module are combined together based on double tracks firstly so that the DNA 4-10 decoder is realized; and the output result is enabled to be more stable by regulating the concentration coefficient of the threshold gate in the fan-out gate module, the AND gate module and the OR gate module.

Description

technical field [0001] The invention relates to the field of DNA strand replacement, in particular to a method for designing a stable DNA4-10 decoder using dual-track logic and an AND module and an OR module designed by Winfree. Background technique [0002] In recent decades, the position of DNA nanotechnology in bioengineering has become more and more important, and DNA strand displacement, as one of the branches of DNA nanotechnology, has also been widely used in the design of various complex biological devices and enable complex DNA computing, thanks in large part to the autonomy of the DNA strand displacement mechanism: once the signal chain and the gate are mixed together, the interaction is performed without outside interference until the signal chain Or the gate is consumed; the impetus for the underpinning reaction comes from the structure of the gate and Watson-Crick (C-G,T-A) complementarity, without the need for enzymes, transcription or translation components. ...

AI Technical Summary

Problems solved by technology

The combination of these three modules and the double track can theoretically design any type of DNA logic circuit. However, due to the reversibility of the DNA strand displacement reaction and the complex factors affecting the reaction, the design of the DNA logic circuit still has a certain degree of theory and experiment. The gap, which leads to a large number of complex DNA logic circuits have not been designed

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