Suturing Toehold activation method used for controlling DNA strand displacement reaction and toolkit
A technology of displacement reaction and DNA chain, applied in the field of molecular biology, can solve the problems of limiting the design flexibility of dynamic DNA nanodevices
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Embodiment 1
[0027] Experimental part of embodiment 1
[0028] 1.1 Materials and Instruments
[0029] All DNA sequences were synthesized by Shanghai Bioengineering Co., Ltd. (China). Mercury nitrate dissolved in 2-5% nitric acid was purchased from Bailingwei Chemical Technology Co., Ltd. (China). ATP, CTP, GTP, UTP and 40% acrylamide, ammonium persulfate, TEMED were all purchased from Shanghai Bioengineering Co., Ltd. (China). All reagents were of analytical grade, and the water used was ultrapure water. All fluorescence experiments were performed on a Japanese Hitachi FL-7000 fluorescence spectrophotometer. Spectral conditions: the excitation voltage is 700V, the excitation wavelength and emission wavelength are 495nm and 530nm, respectively, and the slit width is 10nm. 1.2 Probe Preparation for Binding-Induced Suture Toehold Strand Displacement Reaction
[0030] To prepare Hg 2+ To induce suture RS in Toehold-mediated strand displacement reaction, add 10 μL, 10 μM FAM-labeled TB an...
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