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Rapid culture method of Bursaphelenchus xylophilus endoparasitic fungus Esteya vermicola

A technology of pine wood nematodes and cultivation methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of easy contamination, high cultivation cost, and short storage time, and achieve shortened cultivation time, The effect of long storage time and convenient transportation

Active Publication Date: 2019-04-12
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the main methods for cultivating the endoparasitic fungus Esteya vermicola of pine wood nematode are PDA solid culture and PDB liquid culture, but the problems of these methods are: less spore production, short storage time, easy to be polluted, and high cost of culture

Method used

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  • Rapid culture method of Bursaphelenchus xylophilus endoparasitic fungus Esteya vermicola
  • Rapid culture method of Bursaphelenchus xylophilus endoparasitic fungus Esteya vermicola
  • Rapid culture method of Bursaphelenchus xylophilus endoparasitic fungus Esteya vermicola

Examples

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Embodiment 1

[0040] The present embodiment provides a kind of culture medium of fungus Esteya vermicola, and its preparation method is as follows:

[0041] One group of rice medium formula is divided into: rice 50g, distilled water 50ml, yeast powder 0.5g, KH 2 PO 4 0.05g, natural pH value.

[0042] Weigh 50g of brown rice and invert a 500ml Erlenmeyer flask; then weigh 0.5g of yeast powder, KH 2 PO 4 0.05g, and pour it into a Erlenmeyer flask containing 50g of rice; finally, pour 50ml of sterilized water; shake the Erlenmeyer flask gently to mix the contents evenly; in this example, a total of 3 bottles of culture medium were prepared. Place the prepared culture medium in a sterilizing pot and sterilize at 121°C for 20 minutes.

[0043] Take the fresh slant culture of the fungus Esteya vermicola to prepare the bacterial solution. Inoculated on the above medium. Cool the sterilized medium at room temperature for 15 minutes, and then inoculate it on the ultra-clean workbench. First, a...

Embodiment 2

[0048] The present embodiment provides a new formula of fungus Esteya vermicola rice culture medium, and its preparation method is as follows:

[0049] The two components of the rice medium formula are: rice 50g, distilled water 50ml, and natural pH.

[0050] Weigh 50g of brown rice, invert a 500ml Erlenmeyer flask, and pour 50ml of sterilized water into it; shake the Erlenmeyer flask gently to mix the contents evenly; in this example, a total of 3 bottles of culture medium were prepared. Place the prepared culture medium in a sterilizing pot and sterilize at 121°C for 20 minutes.

[0051] Take the fresh slant culture of the fungus Esteya vermicola to prepare the bacterial solution. Inoculated on the above medium. Cool the sterilized medium at room temperature for 15 minutes, and then inoculate it on the ultra-clean workbench. First, add an appropriate amount of sterilized water to the PDA medium that has cultivated the biocontrol bacterium Esteya vermicola, and then gently ...

Embodiment 3

[0056] This implementation example provides the liquid culture process of the fungus Esteya vermicola PDB and the germination situation of the bacteria, and its preparation method is as follows:

[0057] PDA medium (g medium (over): natural pH value, water 1L, glucose 20g, potato 200g, agar 18g.

[0058] Sterilize the prepared PDA medium at 121°C for 20 minutes. After sterilization, under aseptic conditions, take a 5mm-sized bacterial block from the edge of the plate where fungal colonies grow vigorously, and inoculate it in the middle of the PDA medium. Seal with parafilm. First culture in a constant temperature incubator at 26°C for 2 weeks;

[0059] Preparation of fungal suspension: Pour 1 LPDB liquid medium prepared into four 500mL Erlenmeyer flasks, pour 250mL into each Erlenmeyer flask, and sterilize by high-pressure steam at 121°C for 20min. Secondly, the fungi activated by PDA culture were transferred to PDB medium, and then cultured with shaking on a constant temper...

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Abstract

The invention discloses a rice medium formula and culture method of Bursaphelenchus xylophilus endoparasitic fungus Esteya vermicola, and belongs to the technical field of microorganisms. Compared with a conventional PDA solid culture method and a PDB liquid culture method, a rice medium preparation and culture method of the Bursaphelenchus xylophilus endoparasitic fungus Esteya vermicola not onlyshortens the culture time of the bacteria, but also obtains a high-purity, high-density fungal spore suspension. The method is applied to the control of Bursaphelenchus xylophilus, and has significant economic, ecological and social benefits.

Description

technical field [0001] The invention relates to a method for preparing and cultivating a rice culture medium of the endoparasitic fungus Esteya vermicola of pine wood nematode. Background technique [0002] Pine wood nematode disease, also known as pine wilt disease, is a devastating epidemic of pine trees, which can cause rapid withering and death of pine forests, causing serious damage to forest resources and causing great losses to the national economy. quarantine diseases. The causative agent of pine wood nematode disease is Bursaphelenchus xylophilus, which spreads through human factors and medium beetles. [0003] At present, the prevention and control of pine wood nematode disease is mainly carried out from the two aspects of its pathogenic pine wood nematode and the transmission medium Monochamus alternatus. Failed to really control and eradicate the infected areas. At present, the biological control of pine wood nematode at home and abroad is mainly realized by u...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 马涛朱映贺英豪王偲文超张锦坤蓝来娇温秀军
Owner SOUTH CHINA AGRI UNIV
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